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[1]杨 春,李 恒,董欣宇,等.姜烯酚6衍生物M14通过调控Keap1/Nrf2信号通路诱导人结直肠癌HCT-8细胞凋亡[J].宁夏医科大学学报,2021,(02):109-112.[doi:10.16050/j.cnki.issn1674-6309.2021.02.001]
 YANG Chun,LI Heng,DONG Xinyu,et al.Gingerol 6 Derivative M14 Induces Human Colorectal Cancer HCT-8 Cells Apoptosis through Keap1/Nrf2 Signaling Pathway[J].Ningxia Medical University,2021,(02):109-112.[doi:10.16050/j.cnki.issn1674-6309.2021.02.001]
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姜烯酚6衍生物M14通过调控Keap1/Nrf2信号通路诱导人结直肠癌HCT-8细胞凋亡(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2021年02期
页码:
109-112
栏目:
基础医学
出版日期:
2021-02-28

文章信息/Info

Title:
Gingerol 6 Derivative M14 Induces Human Colorectal Cancer HCT-8 Cells Apoptosis through Keap1/Nrf2 Signaling Pathway
文章编号:
1674-6309(2021)02-0109-04
作者:
杨 春 李 恒 董欣宇 谢小亮 张 东
(宁夏医科大学总医院结直肠外科,银川 750004)
Author(s):
YANG Chun LI Heng DONG Xinyu XIE Xiaoliang ZHANG Dong
(Department of Colorectal Surgery,the General Hospital of Ningxia Medical University,Yinchuan 750004,China)
关键词:
姜烯酚6衍生物M14人结直肠癌HCT-8细胞细胞凋亡Keap1/Nrf2信号通路
Keywords:
Gingerol 6 derivative M14human colorectal cancer HCT-8 cellapoptosisKeap1/Nrf2 signaling pathway
分类号:
R735.3+5
DOI:
10.16050/j.cnki.issn1674-6309.2021.02.001
文献标志码:
A
摘要:
目的 探讨姜烯酚6衍生物 M14 (S6-M14)诱导人结直肠癌HCT-8细胞凋亡的作用及可能的分子机制。方法 MTT 法检测不同浓度的姜烯酚6衍生物 M14 对人结直肠癌HCT-8细胞增殖的影响;ATP显色法检测姜烯酚6衍生物 M14促进人结直肠癌HCT-8细胞凋亡的剂量依赖效应;Western blot检测胞质内 Keap1和 Nrf2蛋白及核内Nrf2蛋白的表达。结果 0.25、0.5、1、2、4 mmol·L-1 S6-M14均可抑制人结直肠癌HCT-8细胞的增殖(P均<0.001);姜烯酚6衍生物M14剂量依赖性地诱导人结直肠癌HCT-8细胞凋亡;姜烯酚6衍生物M14下调人结直肠癌HCT-8细胞胞质内Keap1和Nrf2蛋白表达,促进Nrf2蛋白的核易位(P均<0.05)。结论 姜烯酚6衍生物M14抑制人结直肠癌HCT-8细胞增殖、诱导细胞凋亡,调控Keap1/Nrf2信号通路,有潜在的预防结直肠癌发生的作用。
Abstract:
Objective To study the effect of Gingerol 6 derivative M14(S6-M14)on human colorectal cancer HCT-8 cell apoptosis and its possible molecular mechanism. Methods MTT assay was used to determine the effect of different concentrations of S6-M14 on the activity of human colorectal cancer HCT-8 cells. The dose-dependent effect of S6-M14 on the apoptosis of human colorectal cancer HCT-8 cells was detected by The ATP chromogenic method. Keap1/Nrf2 signaling pathway protein expression was analyzed by Western blot. Results 0.25,0.5,1,2 and 4 mmol·L-1 S6-M14 all inhibited the activity of human colorectal cancer HCT-8 cell(P all<0.001). S6-M14 dose-dependently inducted apoptosis of human colorectal cancer HCT-8 cell. S6-M14 down-regulates the expression of Keap1 and Nrf2 proteins in the cytoplasm of human colorectal cancer HCT-8 cells, and promotes Nrf2 protein nuclear translocation(P all<0.05). Conclusion S6-M14 can prevent colon cancer by inhibiting human colorectal cancer HCT-8 cell proliferation, inducing apoptosis, and regulating Keap1/Nrf2 signaling pathway.

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备注/Memo

备注/Memo:
收稿日期:2020-01-01
基金项目:宁夏医科大学优势学科群项目(XY201509;XY201507)
作者简介:杨春(1973—),硕士,主任医师,副教授,硕士研究生导 师,从事结直肠肿瘤的发生发展、治疗及营养、预后研究等。 E-mail:yangchunln@126.com
通信作者:张东(1961—),主任医师,从事结直肠肿瘤的发生发展、 治疗及预后研究。E-mail:13909590066@163.com
更新日期/Last Update: 1900-01-01