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[1]黄燕飞,黑常春,龚歆,等.盐酸法舒地尔对急性癫痫大鼠海马组织氧化应激因子及凋亡相关因子表达的作用[J].宁夏医科大学学报,2020,(08):770-775.[doi:10.16050/j.cnki.issn1674-6309.2020.08.003]
 HUANG Yanfei,HEI Changchun,GONG Xin,et al.Protective Effects of Fasudil Hydrochloride on Hippocampus of Brain by Reducing Oxidative Stress and Apoptosis of Rat Model of Epilepsy[J].Ningxia Medical University,2020,(08):770-775.[doi:10.16050/j.cnki.issn1674-6309.2020.08.003]
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盐酸法舒地尔对急性癫痫大鼠海马组织氧化应激因子及凋亡相关因子表达的作用(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2020年08期
页码:
770-775
栏目:
论著
出版日期:
2021-04-17

文章信息/Info

Title:
Protective Effects of Fasudil Hydrochloride on Hippocampus of Brain by Reducing Oxidative Stress and Apoptosis of Rat Model of Epilepsy
文章编号:
1674-6309(2020)08-0770-06
作者:
黄燕飞1黑常春2龚歆2任双来3李媛媛1
1. 宁夏回族自治区人民医院全科医学科,银川 750021; 2. 宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川 750004; 3. 宁夏医科大学颅脑疾病重点实验室,银川 750004
Author(s):
HUANG Yanfei1 HEI Changchun2 GONG Xin2 REN Shuanglai3 LI Yuanyuan1
1. Department of General Medicine,People’s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750021,China; 2. Department of Anatomy and Histology of Basic Medical School,Ningxia Medical University, Yinchuan 750004,China; 3. Ningxia Key Laboratory of Craniocerebral Diseases, Yinchuan 750004,China
关键词:
大鼠癫痫盐酸法舒地尔氧化应激凋亡
Keywords:
rat fasudil hydrochloride hippocampus oxidative stress apoptosis
分类号:
R742.1
DOI:
10.16050/j.cnki.issn1674-6309.2020.08.003
文献标志码:
A
摘要:
目的 探讨盐酸法舒地尔对癫痫大鼠大脑海马神经元氧化应激及凋亡的保护作用。方法 将30只SD大鼠随机分成3组,分别为对照组、癫痫模型组(模型组)和盐酸法舒地尔处理组(治疗组),每组10只。采用氯化锂-匹罗卡品诱发癫痫大鼠模型,造模成功后予盐酸法舒地尔10 mg·kg-1腹腔注射治疗,每天1次。采用HE和尼氏染色法观察各组海马组织和神经细胞形态变化,ELISA检测各组大脑海马组织活性氧(ROS)、超氧化物歧化酶1(SOD1)、谷胱甘肽(GSH)活性及丙二醛(MDA)含量的变化, Real-time PCR检测各组大鼠大脑海马组织Bcl-2及Caspase-3基因表达,Western blot检测各组海马中BCL-2及Caspase-3蛋白表达水平。结果 HE染色显示对照组和治疗组两侧海马无明显损伤,海马CA1区及门区细胞排列整齐,形态完整,模型组海马CA1及门区细胞排列不规则。尼氏染色结果显示治疗组两侧海马组织CA1 区神经细胞计数增加(P<0.05)。ELISA检测结果显示治疗组较模型组相比海马组织SOD1和GSH活性增加,ROS和MDA含量降低(P<0.05);Real-time PCR和Western blot检测结果显示Caspase-3基因和蛋白表达减少,BCL-2基因和蛋白表达增加(P均<0.05)。结论 盐酸法舒地尔抑制氧化应激和降低凋亡的发生,从而对癫痫大鼠脑组织起到抗氧化和抗凋亡作用,具有神经保护的作用。
Abstract:
Objective To investigate the protective effect of Fasudil hydrochloride on oxidative stress and apoptosis of Kainic acid-induced Epilepsy model of rat and the intervention effect of fasudil hydrochloride on the related functional changes. Methods Thirty SD rats were randomly divided into three groups: control group, epilepsy model group (model group) and fasudil hydrochloride treatment group (treatment group),with 10 rats in each group. The epileptic rat model was induced by lithium pilocarpine chloride. After the model was established, fasudil hydrochloride was injected intraperitoneally once a day. The morphological changes of hippocampal tissues and nerve cells were observed by HE and Nissl’s staining. The activities of reactive oxygen species (ROS),superoxide dismutase-1(SOD1),glutathione(GSH)and malondialdehyde(MDA) were detected by ELISA. The expression of Bcl-2 and Caspase-3 in hippocampus was detected by real-time PCR. The expression levels of BCL-2 and Caspase-3 in hippocampus were detected by Western blot. Results HE staining showed that there was no obvious damage to both sides of the hippocampus in the control group and the treatment group, and the cells in the CA1 area and portal area of the hippocampus were arranged orderly and intact, while the cells in the CA1 and portal area of the hippocampus in the model group were arranged irregularly. Nissl’s staining results showed that the number of neurons in CA1 area of hippocampus increased in the treatment group(P<0.05). The results of ELISA showed that compared with the model group, the activities of SOD1 and GSH were increased, the contents of ROS and MDA were decreased(P<0.05);the expression of Caspase-3 gene and protein was decreased, and the expression of BCL-2 gene and protein was increased(P all< 0.05). Conclusion The fasudil hydrochloride inhibits oxidative stress and reduces the occurrence of apoptosis,thus it has a neuroprotective effect against oxidative stress and apoptotic on the brain tissue of epileptic in model rats.

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备注/Memo

备注/Memo:
收稿日期:2019-11-17
基金项目:陕西省社会发展科技攻关项目(S2015YFSF0162)
作者简介:刘丽(1986—),山西人,硕士,主治医师,研究方向:小儿肾脏及内分泌疾病。E-mail:164539728@qq.com
通信作者:黄燕萍(1963—),陕西人,博士,主任医师,研究方向:小儿肾脏及内分泌疾病。E-mail:huangyp423@126.com
更新日期/Last Update: 2020-08-20