|本期目录/Table of Contents|

[1]段相国,周秋楠,马 斌,等.阿司匹林对结肠癌SW480细胞增殖和迁移的影响[J].宁夏医科大学学报,2019,(12):1232-1236.[doi:10.16050/j.cnki.issn1674-6309.2019.12.009]
 DUAN Xiangguo,ZHOU Qiunan,MA Bin,et al.Effect of Aspirin on the Biological Function of Colon Cancer SW480 Cells[J].Ningxia Medical University,2019,(12):1232-1236.[doi:10.16050/j.cnki.issn1674-6309.2019.12.009]
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2019年12期
页码:
1232-1236
栏目:
论著
出版日期:
2020-03-27

文章信息/Info

Title:
Effect of Aspirin on the Biological Function of Colon Cancer SW480 Cells
文章编号:
1674-6309(2019)12-1232-05
作者:
段相国12 周秋楠1 马 斌3 张 东4 苏春霞5 夏羽菡6
(1. 宁夏医科大学临床医学院医学检验学系,银川 750004; 2. 宁夏医科大学总医院外科学研究室,银川 750004; 3. 银川市第一人民医院肿瘤外科,银川 750001; 4. 宁夏医科大学总医院结直肠外科,银川 750004; 5. 宁夏医科大学基础医学院病原生物学与免疫学系,银川 750004; 6. 宁夏医科大学总医院营养科,银川 750004)
Author(s):
DUAN Xiangguo12 ZHOU Qiunan1 MA Bin3 ZHANG Dong4 SU Chunxia5 XIA Yuhan6
(1. Department of Clinical Laboratory Medicine, College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, China;2. Department of surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China;3. Department of Oncology Surgery, The First People’s Hospital of Yinchuan, Yinchuan 750001,China;4. Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China;
关键词:
阿司匹林结直肠癌细胞细胞增殖凋亡迁移侵袭
Keywords:
aspirincolorectal cancer cellcell proliferationapoptosisinvasionmigration
分类号:
R735.3+5
DOI:
10.16050/j.cnki.issn1674-6309.2019.12.009
文献标志码:
A
摘要:
目的 探讨阿司匹林(aspirin,ASA)对人结肠癌SW480细胞增殖、迁移和侵袭能力的影响。方法 采用人结肠癌SW480细胞株,将0、1、2和4 mmol·L-1的ASA与细胞共培养作用24、48和72 h,通过CCK8实验观察ASA对SW480细胞增殖能力的影响;通过划痕实验、Transwell实验研究ASA对SW480细胞迁移、侵袭能力的影响;通过Western blot检测凋亡相关蛋白BAX、Bcl-2和肿瘤侵袭、迁移相关蛋白FAK、MMP9的表达情况。结果 在同一时间下,与对照组相比,随着ASA作用浓度的增加SW480细胞的存活率降低(P<0.05);在同一浓度下,与对照组相比,随着ASA作用时间的增加,SW480细胞的存活率降低(P<0.05)。与对照组(0 mmol·L-1)相比,ASA能抑制结肠癌细胞增殖,降低Bcl2表达,提高Bax表达(P均<0.05);对SW480细胞迁移能力产生抑制(P<0.05),并下调迁移和侵袭相关蛋白FAK和MMP9的表达(P均<0.05)。结论 ASA对结肠癌SW480细胞的迁移和侵袭能力有抑制作用,其抑制作用与FAK和MMP9蛋白表达下降有关。
Abstract:
Objective To investigate the effects of aspirin (ASA) on the proliferation, migration and invasion of human colon cancer SW480 cells. Methods Human colon cancer cell line SW480 was co-cultured with 0, 1, 2 and 4 mmol·L-1 aspirin at different time points for 24,48 h and at 72 h, the effects of the drug on the proliferation of colon cancer SW480 cells were observed by CCK8 experiment.The effects of aspirin on the migration and invasion ability of SW480 cells were studied by scratch test, transwell cell migration and invasion experiments. The expression of apoptosis-related proteins Bax, Bcl-2, tumor invasion and migration related proteins FAK and MMP9 were detected by Western blot. Results At the same time, compared with the control group, the survival rate of SW480 cells decreased with the increase of aspirin concentration(P<0.05). At the same concentration, compared with the control group, with the increase of aspirin treatment time, the survival rate of SW480 cells decreased(P<0.05). Compared with the control group (0 mmol ·L-1), aspirin could inhibit the proliferation of colon cancer cells, reduce the expression of BCL2, increase the expression of Bax(P all<0.05), inhibit the migration of SW480 cells(P<0.05),and down regulate the expression of FAK and MMP9 (P all<0.05). Conclusion Aspirin can inhibit the proliferation of colon cancer SW480 cells. Aspirin can induce apoptosis and promote the expression of BAX. Aspirin can inhibit the migration and invasion of colon cancer SW480 cells. Its inhibitory effect is related to the expression of FAK and MMP9 proteins in SW480 cells.

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备注/Memo

备注/Memo:
收稿日期:2019-01-14
基金项目:国家自然科学基金(81760437);宁夏自治区“十三五”重点研发计划(重大科技项目)(2016BZ02);教育部春晖计划项目(Z2016045);留学回国人员创新创业项目(宁人社函〔2017〕84号);宁夏回族自治区重点研发计划一般项目(2018BEG03073)
作者简介:段相国(1974-),男,副教授,硕士研究生导师,主要从事肿瘤免疫相关研究。
通信作者:夏羽菡,副主任药师,主要从事临床营养药学研究。 E-mail:xyhl
作者单位:5. Department of Pathogen Biology and Immunology, School of Basic Medical Science, Ningxia Mmedical University, Yinchuan 750004,China;
     6. Department of Nutrition, the General Hospital of Ningxia Medical University,Yinchuan 750004,China)
更新日期/Last Update: 2019-12-30