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[1]孙书豪,巩发海,彭根远,等.CXCL10/CXCR3轴对结直肠癌SW480、SW620细胞侵袭及上皮间质化的影响[J].宁夏医科大学学报,2019,(06):541-545,560.[doi:10.16050/j.cnki.issn1674-6309.2019.06.001]
 SUN Shuhao,GONG Fahai,PENG Genyuan,et al.Effect of CXCL10/CXCR3 Axis on Invasion and EpithelialInterstitialization of Colorectal Cancer SW480 and SW620 Cells[J].Ningxia Medical University,2019,(06):541-545,560.[doi:10.16050/j.cnki.issn1674-6309.2019.06.001]
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CXCL10/CXCR3轴对结直肠癌SW480、SW620细胞侵袭及上皮间质化的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2019年06期
页码:
541-545,560
栏目:
论著
出版日期:
2019-06-30

文章信息/Info

Title:
Effect of CXCL10/CXCR3 Axis on Invasion and EpithelialInterstitialization of Colorectal Cancer SW480 and SW620 Cells
文章编号:
1674-6309(2019)06-0541-05
作者:
孙书豪1 巩发海1 彭根远1 陈 托1 李 海2
(1. 宁夏医科大学临床医学院, 银川 750004; 2. 宁夏医科大学总医院结直肠外科, 银川 750004)
Author(s):
SUN Shuhao1 GONG Fahai1 PENG Genyuan2 CHEN Tuo2 LI Hai2
(1. Clinical Medical College of Ningxia Medical University, Yinchuan 750004, China; 2. Department of Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004, China)
关键词:
CXCL10/CXCR3轴 结直肠肿瘤上皮间质转化
Keywords:
CXCL10/CXCR3colorectal neoplasms epithelial-mesenchymal transition
分类号:
R735.34
DOI:
10.16050/j.cnki.issn1674-6309.2019.06.001
文献标志码:
A
摘要:
目的 明确CXCL10/CXCR3轴对于结直肠癌SW480、SW620细胞迁移、浸润功能的影响。探讨CXCL10/CXCR3对于结直肠癌SW480、SW620细胞上皮间质转化(EMT)的影响。方法 抑制CXCR3的SW480、SW620细胞经CXCL10刺激后,采用Transwell实验方法测定细胞迁移、浸润能力的改变,Western blot测定细胞EMT相关蛋白表达量的变化。结果 趋化因子CXCL10可以促进肿瘤细胞SW480和SW620的迁移浸润(P<0.01),而当抑制趋化因子受体CXCR3时,这种促肿瘤迁移、浸润的能力明显减弱(P<0.01)。趋化因子CXCL10可以促进SW480、SW620间质细胞标志物例如N-钙黏素(N-cadherin)和波形蛋白(Vimentin)的表达(P<0.01),可以抑制SW620细胞上皮细胞标志物E-钙黏素(E-cadherin)的表达(P<0.01),而对SW480细胞的E-cadherin无明显的影响。病毒转染抑制CXCR3受体后,CXCL10的这种促进间质细胞标志物N-cadherin和Vimentin的表达能力消失甚至逆转,并且促进SW480和SW620细胞系上皮细胞标志物E-cadherin的表达(P<0.01)。结论 CXCL10有促进肿瘤细胞迁移、浸润的作用,促进结直肠肿瘤细胞间质细胞标志物的表达,同时抑制上皮细胞标志物的表达。
Abstract:
Objective To determine the effects of CXCL10/CXCR3 axis on migration and invasion of SW480 and SW620 cells. And to investigate the effect of CXCL10/CXCR3 on epithelial-mesenchymal transition (EMT) of colorectal cancer SW480 and SW620 cells. Methods Transwell assay was used to determine the migration and infiltration ability of SW480 and SW620 cells after CXCL10 stimulation and inhibition by CXCR3. The expression of EMT-related proteins in CXCL10 and CXCR3 was detected by Western blot. Results Chemokine CXCL10 could stimulate the migration and invasion of tumor cells SW480 and SW620(P<0.01),while the ability to promote tumor invasion was significantly reduced or even reversed when the chemokine receptor CXCR3 was inhibited(P<0.01). Chemokine CXCL10 could promote the expression of SW480 and SW620 interstitial cell markers such as N-cadherin and Vimentin(P<0.01),and inhibit the expression of epithelial cell marker E-cadherin(P<0.01). E-cadherin had no significant effect. After viral transfection inhibited the CXCR3 receptor,the expression of N-cadherin and Vimentin disappeared or even reversed,and the expression of E-cadherin was higher in SW480 and SW620 cell lines(P<0.01). Conclusion CXCL10 has a role in promoting migration and invasion of tumor cells. Inhibition of CXCR3 receptor can abolish the promotion of CXCL10 on tumor cell migration and invasion. CXCL10 can promote the expression of cell mesenchymal cell markers in colorectal tumors while inhibiting the expression of epithelial cell markers. new clinical basis for the prevention and treatment of DVT after orthopedic surgery in hypertensive patients.

参考文献/References:

[1] Torre LA,Bray F,Siegel RL,et al. Global cancer statistics,2012[J]. CA Cancer J Clin,2015,65(2):87-108.
[2] Triantafillidis JK,Nasioulas G,Kosmidis PA. Colorectal cancer and inflammatory bowel disease: epidemiology,risk factors,mechanisms of carcinogenesis and prevention strategies[J]. Anticancer Res,2009,29(7):2727-2737.
[3] Dufour JH,Dziejman M,Liu MT,et al. IFN-gamma-inducible protein 10 (IP-10;CXCL10)-deficient mice reveal a role for IP-10 in effector T cell generation and trafficking [J]. J Immunol,2002,168(7):3195-3204.
[4] Melik-Parsadaniantz S,Rostene W. Chemokines and neuromodulation[J]. J Neuroimmunol,2008,198(1-2):62-68.
[5] Bai M,Chen X,Ba YI. CXCL10/CXCR3 overexpression as a biomarker of poor prognosis in patients with stage II colorectal cancer[J]. Mol Clin Oncol,2016,4(1):23-30.
[6] Wu Z,Han X,Yan J,et al. The prognostic significance of chemokine receptor CXCR3 expression in colorectal carcinoma[J]. Biomed Pharmacother,2012,66(5):373-377.
[7] Mackay CR. Chemokines: immunology’s high impact factors[J]. Nat Immunol,2001,2(2):95-101.
[8] Youn BS, Mantel C, Broxmeyer HE. Chemokines, chemokine receptors and hematopoiesis[J]. Immunol Rev,2000,177:150-174.
[9] Nieto MA,Huang RY,Jackson RA,et al. EMT: 2016[J]. Cell,2016,166(1):21-45.
[10] Qin Y,Capaldo C,Gumbiner BM,et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin[J]. J Cell Biol,2005,171(6):1061-1071.
[11] Portadelariva M,Stanisavljevic J,Curto J,et al. TFCP2c/LSF/LBP-1c is required for Snail1-induced fibronectin gene expression[J]. Biochem J,2011,435(3):563-568.
[12] Zeng YJ,Lai W,Wu H,et al. Neuroendocrine-like cells -derived CXCL10 and CXCL11 induce the infiltration of tumor-associated macrophage leading to the poor prognosis of colorectal cancer[J]. Oncotarget,2016,7(19):27394-27407.
[13] Cambien B,Karimdjee BF,Richard-Fiardo P, et al. Organ-specific inhibition of metastatic colon carcinoma by CXCR3 antagonism[J]. Br J Cancer, 2009,100(11):1755-1764.
[14] Murakami T,Kawada K,Iwamoto M,et al. The role of CXCR3 and CXCR4 in colorectal cancer metastasis[J]. Int J Cancer,2013,132(2):276-287.
[15] Zhou H,Wu J,Wang T,et al. CXCL10/CXCR3 axis promotes the invasion of gastric cancer via PI3K/AKT pathway-dependent MMPs production[J]. Biomed Pharmacother,2016,82:479-488.
[16] Ren T,Zhu L,Cheng M. CXCL10 accelerates EMT and metastasis by MMP-2 in hepatocellular carcinoma[J]. Am J Transl Res,2017,9(6):2824-2837.
[17] Tokunaga R,Zhang W,Naseem M,et al. CXCL9, CXCL10,CXCL11/CXCR3 axis for immune activation A target for novel cancer therapy[J]. Cancer Treatment Reviews,2018,63:40-47.

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备注/Memo

备注/Memo:
收稿日期:2019-04-05
基金项目:宁夏医科大学优势学科群科研项目(XY201821)
作者简介:孙书豪(1992-),男,陕西人,在读硕士研究生,主要从事消化道肿瘤研究。E-mail:newssh00@163.com
通信作者:李海,男,硕士研究生导师,副教授。E-mail: Zhuoran1126@163.com
更新日期/Last Update: 2019-06-30