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[1]赵启跃,姚 遥,郑 萍,等.黄芪甲苷对阿尔茨海默症小鼠脑内氧化应激和NADPH氧化酶蛋白表达的影响[J].宁夏医科大学学报,2018,(11):1241-12,441,249.[doi:10.16050/j.cnki.issn1674-6309.2018.11.001]
 ZHAO Qiyue,YAO Yao,ZHENG Ping,et al.Effects of Astragaloside IV on Oxidative Stress Level and Protein Expression of NADPH Oxidase in Lipopolysaccharides Induced Alzheimer's Mice Model[J].Ningxia Medical University,2018,(11):1241-12,441,249.[doi:10.16050/j.cnki.issn1674-6309.2018.11.001]
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黄芪甲苷对阿尔茨海默症小鼠脑内氧化应激和NADPH氧化酶蛋白表达的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年11期
页码:
1241-12,441,249
栏目:
论 著
出版日期:
2018-11-30

文章信息/Info

Title:
Effects of Astragaloside IV on Oxidative Stress Level and Protein Expression of NADPH Oxidase in Lipopolysaccharides Induced Alzheimer's Mice Model
文章编号:
1674-6309(2018)11-1241-04
作者:
赵启跃1 姚 遥2 郑 萍34 李 南3 王 伟3 韩欣益3 李 娟35
(1. 延安大学附属医院临床药学科,延安 716000; 2. 宁夏医科大学基础医学院,银川 750004; 3. 宁夏医科大学药学院,银川 750004; 4. 宁夏回药现代化工程技术研究中心,银川 750004; 5. 回医药现代化教育部重点实验室,银川 750004)
Author(s):
ZHAO Qiyue1 YAO Yao2 ZHENG Ping34 LI Nan3 WANG Wei3 HAN Xinyi3 LI Juan35
(1. Department of Clinical Pharmacy, Affiliated Hospital of Yan'an University, Yan'an 716000; 2. School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004; 3. School of Pharmacy, Ningxia Medical University, Yinchuan 750004; 4. Ningxi
关键词:
黄芪甲苷阿尔茨海默症脂多糖氧化应激NADPH氧化酶
Keywords:
astragaloside IV Alzheimer's disease lipopolysaccharides oxidative stress NADPH oxidase
分类号:
R96
DOI:
10.16050/j.cnki.issn1674-6309.2018.11.001
文献标志码:
A
摘要:
目的 探讨黄芪甲苷对脂多糖(LPS)诱导的阿尔茨海默症(Alzheimer’s disease,AD)小鼠模型脑组织内氧化应激水平和NADPH氧化酶蛋白表达的影响。方法 60只雄性ICR小鼠随机分为6组:空白对照组,模型对照组,黄芪甲苷低、中、高剂量组和阳性对照组,每组10只。除空白对照组外其他各组小鼠侧脑室单次注射LPS建立AD小鼠模型(5?滋g·L-1,3?滋L/只),空白对照组小鼠注射等量的生理盐水。造模次日起,黄芪甲苷低、中、高剂量组小鼠分别给予不同剂量黄芪甲苷(20、40和80mg·kg-1)灌胃,阳性对照组给予多奈哌齐(5mg·kg-1),空白对照组和模型对照组小鼠灌胃生理盐水,灌胃均为每日1次,连续14d。14d后,采用ELISA法检测小鼠脑组织内活性氧(ROS)和丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性;运用Western blot方法检测各组小鼠脑组织中NADPH氧化酶亚基gp91phox和p47phox蛋白含量。结果 与模型对照组相比,黄芪甲苷给药各组以及阳性对照组小鼠脑内ROS、MDA含量均下降,SOD和GSH-Px活性升高(P均<0.01);同时黄芪甲苷能够抑制LPS诱导的AD小鼠脑内gp91phox和p47phox亚基蛋白表达 (P均<0.01)。结论 黄芪甲苷能够降低侧脑室注射LPS诱导的AD小鼠脑内氧化应激水平,这一作用可能与其能抑制NADPH氧化酶关键亚基gp91phox和p47phox蛋白的表达有关。
Abstract:
Objective To investigate the effects of astragaloside IV(AS-IV) on oxidative stress level and protein expression of NADPH oxidase in lipopolysaccharides(LPS) induced Alzheimer’s disease(AD) mice. Methods Sixty male ICR mice were randomly divided into six groups: normal control group, model group, AS-IV low,medium, high dose group and positive control group. Normal control group received intracerebroventricular injection with saline,and the other groups were intracerebroventricularly injected with LPS to establish AD mice model. AS-IV treatment groups were given daily oral gavage with different dose of AS-IV(20mg·kg-1,40mg·kg-1,80mg·kg-1),and normal control group and model group mice were given gavage with equal amount of normal saline from the next day after the AD model established. Donepezil (5mg·kg-1) was used as positive control. After 14 days administration, the levels of ROS,MDA and enzymatic activity of SOD,GSH-Px in mice brain were assessed by ELISA method. The expression of NADPH oxidase subunits gp91phox and p47phox in mice brain were detected by Western blot. Results Biochemical assay revealed that AS-IV could remarkably reduce the content of ROS and MDA, while the activity of SOD and GSH-Px significantly increased(P all<0.01)compared with model group. LPS induced protein expression levels of NADPH oxidase subunits gp91phox and p47phox were also significantly reduced by AS-IV(P all<0.01). Conclusion AS-IV could remarkably ameliorate LPS induced oxidative stress in AD mice brain, which may relate to the inhibition of protein expression of NADPH oxidase subunits gp91phox and p47phox.

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备注/Memo

备注/Memo:
收稿日期:2018-08-12
基金项目: 国家自然科学基金(81660673;81460665),教育部春晖计划项目(Z2016047),宁夏重点研发计划重点项目(2018BFG02005),宁夏高等学校科学研究项目(NGY2015097),大学生创新创业训练计划项目(201510752005;20140331)
作者简介:赵启跃(1981-),女,陕西人,学士,主管药师。
通信作者:李娟,女,博士,副教授,主要从事阿尔茨海默病的研究及其新药研发工作。E-mail:lijuan_0912@qq.com
更新日期/Last Update: 2018-11-30