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[1]姚 杰,刘 斐,高 艳,等.米贝地尔强化加巴喷丁对糖尿病大鼠神经病理性疼痛的镇痛效应[J].宁夏医科大学学报,2018,(08):903-906.[doi:10.16050/j.cnki.issn1674-6309.2018.08.008]
 YAO Jie,LIU Fei,GAO Yan,et al.Mibefradil Potentiates Gabapentin's Efficacy of Rats with Diabetic Neuropathy Pain[J].Ningxia Medical University,2018,(08):903-906.[doi:10.16050/j.cnki.issn1674-6309.2018.08.008]
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米贝地尔强化加巴喷丁对糖尿病大鼠神经病理性疼痛的镇痛效应(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年08期
页码:
903-906
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Mibefradil Potentiates Gabapentin's Efficacy of Rats with Diabetic Neuropathy Pain
文章编号:
1674-6309(2018)08-0903-04
作者:
姚 杰1 刘 斐2 高 艳1 庞茜茜3 郭 颖4 滕金亮1
(1. 河北北方学院附属第一医院麻醉科,张家口 075000; 2. 河北北方学院附属第一医院ICU,张家口 075000; 3. 河北北方学院附属第一医院药学部,张家口 075000; 4. 河北北方学院病理学教研室,张家口 075000)
Author(s):
YAO Jie1 LIU Fei2 GAO Yan1 PANG Qianqian3 GUO Ying4 TENG Jinliang1
(1. Department of Anesthesiology, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000; 2. ICU, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000; 3. Department of Pharmacy, the First Affiliated Hospital o
关键词:
糖尿病神经病理性疼痛米贝地尔加巴喷丁
Keywords:
diabetes mellitusneuropathic painmibefradilgabapentinm
分类号:
R587.1
DOI:
10.16050/j.cnki.issn1674-6309.2018.08.008
文献标志码:
A
摘要:
目的 观察米贝地尔强化加巴喷丁对糖尿病大鼠神经病理性疼痛的镇痛效应。方法 选取健康雄性SD大鼠30只,6~7周龄,体重200~220g,通过腹腔注射链脲佐菌(60mg·kg-1)建立糖尿病神经病理性疼痛模型。将造模成功大鼠随机分为A组(生理盐水组)、B组(加巴喷丁30mg·kg-1组),C组(加巴喷丁60mg·kg-1组)及D组(加巴喷丁30mg·kg-1+米贝地尔10mg·kg-1组),各6只。A组予以生理盐水10mL·kg-1腹腔内注射;B组予以3mg·mL-1的加巴喷丁溶液10mL·kg-1腹腔注射;C组予以6mg·mL-1的加巴喷丁溶液10mL·kg-1腹腔注射;D组予以3mg·mL-1的加巴喷丁溶液+1mg·mL-1的米贝地尔溶液10mL·kg-1腹腔注射。各组大鼠每天每只给药1次。测定比较给药前(T0)、30min(T1)、60min(T2)、120 min(T3)、240min(T4)、480min(T5)时机械缩足阈值(PWT)和热缩足潜伏期(PWL)。结果 与T0比较,C组和D组T1~T5时机械缩足阈值升高(P<0.05)。与A组比较,C和D组机械缩足阈值升高;与B组比较,C和D组机械缩足阈值升高(P<0.05);与C组比较,D组T4时机械缩足阈值升高(P<0.05)。与T0比较, B和C组T1~T3 时热缩足潜伏期均延长(P<0.05)。D组T1~T4时热缩足潜伏期延长(P<0.05)。与A组比较,B和C组T1~T3 时热缩足潜伏期延长;D组T1~T4时热缩足潜伏期延长(P<0.05)。与B组比较,D组T4 时热缩足潜伏期延长,与C组比较,D组T4 时热缩足潜伏期延长(P<0.05)。结论 米贝地尔能够增强加巴喷丁对1型糖尿病大鼠神经病理性疼痛的镇痛效果,减少加巴喷丁用量,延长加巴喷丁有效作用时间。
Abstract:
Objective To investigate the antinociceptive effect of mibefradil potentiates gabapentin's efficacy of rats with diabetic neuropathy pain. Methods Thirty healthy male SD rats, aged 6-7 weeks and weighing 200-220g, were selected. The diabetic neuropathic pain model was established by intraperitoneal injection of streptozotocin(60mg·kg-1). The diabetes mellitus rats were randomly divided into group A(normal saline group),group B(gabapentin 30mg·kg-1 group),group C(gabapentin 60mg·kg-1 group) and group D (gabapentin 30mg·kg-1 + mibapendil 10mg·kg-1 group), with 6 rats in each group. Group A, physiological saline group,was given physiological saline 10mL·kg-1 by intraperitoneal injection;group B,30mg·kg-1 gabapentin group, was given 3mg·mL-1 gabapentin solution 10mL·kg-1 by intraperitoneal injection, qd; group C,60mg·kg-1 gabapentin group,was given 6mg·mL-1 gabapentin solution 10mL·kg-1 by intraperitoneal injection;group D,30mg·kg-1 gabapentin and 10mg·kg-1 mibefradil group, were given 3mg·mL-1 gabapentin solution and 1mg·mL-1 mibefradil solution,10mL·kg-1 by intraperitoneal injection. The paw withdrawal threshold(PWT)and paw withdrawal latency(PWL) were measured before at(T0)and 30min(T1),60min(T2),120min(T3),240min(T4),480min(T5) after administration respectively. Results Compared with T0,the mechanical shrinkage threshold of group C and group D increased from T1 to T5(P<0.05). Compared with group A,the mechanical shrinkage thresholds of group C and D increased, and those of group B increased (P<0.05);and those of group C and D increased at T4(P<0.05). Compared with T0, the latency of heat shrinkable foot in B and C groups was prolonged at T1-T3(P<0.05). In group D, the latent period of heat shrinkable foot was prolonged at T1-T4 (P<0.05). Compared with group A,the latency of febrile foot prolonged at T1-T3 in group B and C,and at T1-T4 in group D(P<0.05). Compared with group B, the latency of febrile foot contraction was prolonged at T4 in group D and at T4 in group C(P<0.05). Conclusion Combination therapy of mibefradil and gabapentin can increase gabapentin's antinociceptive effect in treating type-1 diabetic rats with painful diabetic neuropathy,decrease gabapentin's necessary dosage and prolong gabapentin's effective antinociceptive duration.

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备注/Memo

备注/Memo:
收稿日期:2018-05-14
基金项目:河北省卫生厅科研基金(20170778)
作者简介:姚杰,男,硕士,住院医师,从事临床麻醉工作。
更新日期/Last Update: 2018-08-30