|本期目录/Table of Contents|

[1]袁春秀,刘新兰,吴媛媛.慢病毒介导的Aurora A基因沉默对人胃癌细胞迁移及侵袭的影响[J].宁夏医科大学学报,2018,(08):895-898.[doi:10.16050/j.cnki.issn1674-6309.2018.08.006]
 YUAN Chunxiu,LIU Xinlan,WU Yuanyuan.Effect of Lentivirus-mediated Aurora A Gene Silencing on the Migration and Invasion in Human Gastric Cancer Cell Line[J].Ningxia Medical University,2018,(08):895-898.[doi:10.16050/j.cnki.issn1674-6309.2018.08.006]
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慢病毒介导的Aurora A基因沉默对人胃癌细胞迁移及侵袭的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年08期
页码:
895-898
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Effect of Lentivirus-mediated Aurora A Gene Silencing on the Migration and Invasion in Human Gastric Cancer Cell Line
文章编号:
1674-6309(2018)08-0895-04
作者:
袁春秀 刘新兰 吴媛媛
(宁夏医科大学总医院肿瘤医院肿瘤内科,银川 750004)
Author(s):
YUAN Chunxiu LIU Xinlan WU Yuanyuan
(Department of Medical Oncology, Cancer Hospital of the General Hospital, Ningxia Medical University,Yinchuan 750004)
关键词:
胃癌Aurora A基因细胞迁移及侵袭慢病毒转染
Keywords:
gastric cancer Aurora A gene cell migration and invasionlentivirus infection
分类号:
R735.2
DOI:
10.16050/j.cnki.issn1674-6309.2018.08.006
文献标志码:
A
摘要:
目的 探讨Aurora A基因在人胃癌侵袭、转移中的作用。方法 采用慢病毒感染的方法沉默胃癌SGC-7901细胞Aurora A基因,以细胞划痕实验、迁移实验(Transwell小室)及侵袭实验检测基因沉默后对细胞胃癌细胞迁移和侵袭转移能力的影响。结果 Aurora A基因沉默后的胃癌SGC-7901细胞组24h迁移率和转移细胞及48h侵袭转移细胞数[(0.40±0.03)%、(143.00±4.21)个、(89.00±6.13)个]均低于感染了空载体的胃癌SGC-7901组[(0.51±0.05)%、(171.00±2.11)个、(108.00±4.39)个](P均<0.05)。结论 下调胃癌细胞Aurora A基因表达可降低细胞迁移、侵袭转移能力。
Abstract:
Objective To investigate the effects of lentivirus-mediated Aurora A gene silencing on the migration and invasion in gastric cancer cell. Methods The Aurora A gene of SGC-7901 cells was silenced by lentivirus infection. The effects of gene silencing on the migration, invasion and metastasis of SGC-7901 cells were examined by cell scratch test, migration test(Transwell chamber) and invasion test. Results The 24-hour migration rate,metastatic cells and the number of invasive and metastatic cells at 48h in SGC-7901 cell group with Aurora A gene silencing [(0.40±0.03)%,(143.00±4.21),(89.00±6.13)] were lower than those in SGC-7901 group with empty vector infection [(0.51±0.05)%,(171.00±2.11),(108.00±4.39)] (P<0.05). Conclusion Aurora A gene contribute to the migration and invasion in gastric cancer,down-regulating the expression of Aurora A can inhibit the ability of migration and invasion of cells.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:2018-03-22
基金项目:宁夏自然科学基金(NZ14287)
作者简介:袁春秀(1976-),女,副主任医师,硕士,主要从事肿瘤内科临床与应用基础研究。E-mail:cara2004107@163.com
通信作者:刘新兰,女,硕士研究生导师,主任医师。 E-mail:nxliuxinlan@163.com
更新日期/Last Update: 2018-08-30