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[1]聂黎虹,李亚杰,赵瑞宁,等.脂多糖对人前列腺上皮细胞表达和分泌高迁移率族蛋白B1的影响及意义[J].宁夏医科大学学报,2018,(08):891-894,898.[doi:10.16050/j.cnki.issn1674-6309.2018.08.005]
 NIE Lihong,LI Yajie,ZHAO Ruining,et al.Effect of LPS on the Expression and Secretion of HMGB1 in Human Prostatic Epithelial Cells[J].Ningxia Medical University,2018,(08):891-894,898.[doi:10.16050/j.cnki.issn1674-6309.2018.08.005]
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脂多糖对人前列腺上皮细胞表达和分泌高迁移率族蛋白B1的影响及意义(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年08期
页码:
891-894,898
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Effect of LPS on the Expression and Secretion of HMGB1 in Human Prostatic Epithelial Cells
文章编号:
1674-6309(2018)08-0891-04
作者:
聂黎虹1 李亚杰23 赵瑞宁2 秦凯悦3 单光明23 李 旭23 朱亚洲1
(1. 宁夏医科大学基础医学院生理学系,银川 750004; 2. 宁夏医科大学总医院泌尿外科,银川 750004; 3. 宁夏医科大学,银川 750004)
Author(s):
NIE Lihong1 LI Yajie23 ZHAO Ruining2 QIN Kaiyue3 SHAN Guangming23 LI Xu23 ZHU Yazhou1
(1. Department of Physiology, Ningxia Medical University, Yinchuan 750004; 2. Department of Urology, the General Hospital of Ningxia Medical University, Yinchuan 750004; 3. Ningxia Medical University, Yinchuan 750004)
关键词:
良性前列腺增生高迁移率族蛋白B-1炎症因子
Keywords:
benign prostatic hyperplasia high mobility group box-1 protein cytokines
分类号:
R697+3
DOI:
10.16050/j.cnki.issn1674-6309.2018.08.005
文献标志码:
A
摘要:
目的 探讨脂多糖(LPS)对人前列腺上皮细胞高迁移率族蛋白B-1(high mobility group box-1 protein, HMGB1)表达及分泌的影响及意义。方法 人前列腺上皮细胞株RWPE传代培养,给予LPS(10μg·mL-1)孵育刺激,在刺激不同时间点(3、6、12、24、48h)收集细胞和培养液上清,采用Western blot法检测细胞HMGB1蛋白表达的变化;ELISA法检测培养液上清HMGB1蛋白浓度的变化。给予HMGB1(0、1、10、100、1000ng·mL-1)、LPS、HMGB1阻断剂甘草甜素(100μM,GLY)刺激细胞,采用CCK-8法检测其增殖情况。结果 LPS刺激前列腺上皮细胞3、6、12、24、48h后细胞内HMGB1蛋白相对表达量逐渐增高(F=4.478,P=0.016),以24h蛋白表达水平最高(P<0.01),LPS刺激细胞12、24和48h时HMGB1蛋白表达水平较control组升高(P均<0.05),但12、24和48h这三个时间点之间HMGB1蛋白表达水平差异无统计学意义(P>0.05)。LPS刺激前列腺上皮细胞12、24h后,细胞培养液上清中HMGB1浓度升高(P<0.05),在刺激24h后浓度升高达最高(P<0.01)。给予细胞HMGB1和LPS刺激均可引起细胞增殖能力增强,但给予HMGB1阻断剂GLY后,可使LPS刺激细胞增殖的能力减弱(P<0.05)。结论 致炎因子LPS可增加RWPE细胞HMGB1蛋白的表达,且增多的HMGB1分泌至细胞外可发挥促前列腺上皮细胞增殖的作用。
Abstract:
Objective To investigate the effect of LPS on the expression and secretion of endogenous HMGB1 in prostate epithelial cells. Methods Human prostatic epithelial cell line RWPE was subcultured and incubated with LPS (10?滋g·mL-1). Cell supernatants were collected at different time points(3,6,12,24,48 h) and the expression of HMGB1 protein was detected by Western blot. The concentration of HMGB1 protein in the supernatant was detected by ELISA. Cells were stimulated by HMGB1(0,1,10,100,1000ng·mL-1),LPS,HMGB1 blocker glycyrrhizin(100?滋M,GLY),and the proliferation was detected by CCK-8 method. Results The relative expression of HMGB1 protein in the prostatic epithelial cells was increased after LPS stimulation for 3,6,12,24 and 48h(F=4.478,P=0.016),and the expression of proteinlevel at 24h was the highest(P<0.01). When LPS stimulated cells at 12,24 and 48h, the expression of HMGB1 protein was higher than that of control group(P<0.05),but there was no significant difference in HMGB1 protein expression at the 12,24 and 48h(P>0.05). After LPS stimulated prostate epithelial cells for 12 and 24h,the concentration of HMGB1 in the supernatant of cell culture medium increased(P<0.05),and the concentration increased to the highest after 24h of stimulation(P<0.01). Stimulation of HMGB1 and LPS could induce cell proliferation, the ability of LPS to stimulate cell proliferation was attenuated after HMGB1 blocker GLY was used(P<0.05). Conclusion LPS can increase the expression of HMGB1 protein in RWPE cells,and the increased secretion of HMGB1 to extracellular can promote the proliferation of prostatic epithelial cells.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:2018-03-15
基金项目:宁夏自然科学基金(NZ17071)
作者简介:聂黎虹(1977-),女,宁夏人,副教授,博士,从事生理学教学及科研工作
通信作者:赵瑞宁(1973-),男,山西人,副教授,主任医师,博士,从事泌尿外科临床及科研工作。E-mail: zhaoruining5257@163.com
更新日期/Last Update: 2018-08-30