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[1]田进海,何亚琴,于晶晶,等.MTHFR基因rs1801133 位点多态性与银川市体检人群高血压的相关性分析[J].宁夏医科大学学报,2018,(08):875-880.[doi:10.16050/j.cnki.issn1674-6309.2018.08.002]
 TIAN Jinhai,HE Yaqin,YU Jingjing,et al.Correlation between rs1801133 Polymorphism of MTHFR Gene and Hypertension in Physical Examination Population in Yinchuan[J].Ningxia Medical University,2018,(08):875-880.[doi:10.16050/j.cnki.issn1674-6309.2018.08.002]
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MTHFR基因rs1801133 位点多态性与银川市体检人群高血压的相关性分析(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年08期
页码:
875-880
栏目:
论著
出版日期:
2018-08-30

文章信息/Info

Title:
Correlation between rs1801133 Polymorphism of MTHFR Gene and Hypertension in Physical Examination Population in Yinchuan
文章编号:
1674-6309(2018)08-0875-06
作者:
田进海1 何亚琴2 于晶晶1 马 荣1 周 瑜3 王 嘉1 王立斌1
(1. 宁夏医科大学总医院生物芯片工程中心, 银川 750004; 2.宁夏医科大学总医院干细胞研究所, 银川 750004; 3.宁夏医科大学总医院外科学研究室,银川 750004)
Author(s):
TIAN Jinhai1 HE Yaqin2 YU Jingjing1 MA Rong1 ZHOU Yu3 WANG Jia1 WANG Libin1
(1. Biochip Engineering Center,the General Hospital of Ningxia Medical University,Yinchuan 750004; 2. Stem Cell Institute,the General Hospital of Ningxia Medical University,Yinchuan 750004; 3. Surgery Research Laboratory,the General Hospital of Ningxia M
关键词:
亚甲基四氢叶酸还原酶高血压高分辨率溶解曲线同型半胱氨酸基因分型
Keywords:
methylene tetrahydrofolate reductase hypertension high-resolution melting curve homocysteine genotyping
分类号:
R544.1
DOI:
10.16050/j.cnki.issn1674-6309.2018.08.002
文献标志码:
A
摘要:
目的 探讨高分辨率熔解曲线(hight-resolution melting,HRM)技术在亚甲基四氢叶酸还原酶(MTHFR) 基因分型的应用及rs1801133 位点多态性与银川市体检人群高血压的关联性。方法 选择2017年1-10月间宁夏医科大学总医院体检人群中高血压患者743 例和血压正常者718例为研究对象,收集临床资料,检测血浆同型半胱氨酸(Hcy)、血脂、空腹血糖(FBG)、尿酸等临床生物化学指标。采用HRM 技术对样本进行基因分型。结果 采用HRM 技术成功分型rs1801133位点样本1461例,CC型367例(25.1%)、CT型743例(50.9%)和TT型351例(24.0%),测序验证准确率达100%。银川市人群MTHFR 基因rs1801133位点基因型频率和等位基因频率与天津、山东、江苏及湖北人群差异均有统计学意义(P均<0.01)。rs1801133位点CC、CT和TT基因型间血压和Hcy水平差异有统计学意义(P<0.01)。高血压组rs1801133 的CC、CT、TT三种基因型频率和C、T等位基因频率与正常组比较差异有统计学意义(P<0.01)。高血压组体质量指数(BMI)、FBG、尿酸、Hcy、甘油三酯(TG)及总胆固醇(TC)均高于正常组(P均<0.01)。Logistic回归分析显示,在校正了年龄、BMI、尿酸及Hcy 后rs1801133 不同基因型与高血压具有相关性(P<0.01)。结论 HRM 法是一种检测准确性高、速度快、低成本的方法,适用于MTHFR 基因检测。rs1801133 基因多态性与高血压发病显著相关,且直接影响血浆Hcy 水平。
Abstract:
Objective To explore the application of high-resolution curve(HRM) in methylenetetr-ahydrofolate reductase(MTHFR) genotyping and the association between rs1801133 polymorphism and hypertension in Yinchuan physical examination population. Methods A total of 743 hypertensive patients and 718 controls were collected from physical examination population between January 2017 and October 2017 at General Hospital of Ningxia Medical University. The clinical data were collected and the clinical biochemical indexes such as plasma homocysteine(Hcy),blood lipid, fasting blood glucose (FBG) and uric acid were measured. HRM was used to genotyping the samples. Results HRM technique was used to successfully classify rs1801133 loci in 1461 cases, CC 367(25.1%),CT 743 (50.9%) and TT 351 (24.0%) with 100% accuracy. The genotype frequency and allele frequency of MTHFR gene rs1801133 in Yinchuan were significantly different from those in Tianjin,Shandong, Jiangsu and Hubei(P<0.01). There was a significant difference in blood pressure and Hcy levels among rs1801133 locus CC, CT and TT genotypes(P<0.01). The frequencies of CC, CT and TT genotypes and C, T alleles of rs1801133 in hypertension group were significantly different from those in normal group (P<0.01). Body mass index (BMI), FBG, uric acid, Hcy, triglyceride (TG) and total cholesterol(TC) in hypertension group were higher than those in normal group(P<0.01). Logistic regression analysis showed that different genotypes of rs1801133 were associated with hypertension after adjusting for age, BMI, uric acid and Hcy. Conclusion HRM is a method with high accuracy, high speed and low cost, and is suitable for MTHFR gene detection. Rs1801133 gene polymorphism is significantly related to the incidence of hypertension, and directly affects plasma Hcy level.

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备注/Memo

备注/Memo:
收稿日期:2018-04-19
基金项目:国家自然科学基金(81460152);宁夏医科大学科学研究基金(XZ2016012);宁夏高等学校一流学科建设项目(NXYLXK2017A05)
作者简介:田进海,男,助理研究员,硕士。E-mail: smjinhai@126.com
通信作者:王立斌,博士,副主任技师,硕士研究生导师,研究方向:肿瘤干细胞生物学。E-mail: wanglibin007@126.com
更新日期/Last Update: 2018-08-30