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[1]马丽园,姜 韬,姜 艺,等.超声微泡造影剂携增殖1阻断基因短发夹RNA抑制大鼠肝纤维化的研究[J].宁夏医科大学学报,2018,(06):641-643,653.[doi:10.16050/j.cnki.issn1674-6309.2018.06.005]
 MA Liyuan,JIANG Tao,JIANG Yi,et al.Study of Inhibiting Rats Liver Fibrosis by BOP-1 shRNA withMicrobubble of Ultrasound Contrast Agent[J].Ningxia Medical University,2018,(06):641-643,653.[doi:10.16050/j.cnki.issn1674-6309.2018.06.005]
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超声微泡造影剂携增殖1阻断基因短发夹RNA抑制大鼠肝纤维化的研究(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年06期
页码:
641-643,653
栏目:
论著
出版日期:
2018-06-30

文章信息/Info

Title:
Study of Inhibiting Rats Liver Fibrosis by BOP-1 shRNA with Microbubble of Ultrasound Contrast Agent
作者:
马丽园1 姜 韬2 姜 艺1 王 乐1 杨光飞1 米成嵘1
(1. 宁夏医科大学总医院超声科,银川 750004; 2. 宁夏医科大学总医院结直肠外科,银川 750004)
Author(s):
MA Liyuan1 JIANG Tao2 JIANG Yi1 WANG Le1 YANG Guangfei1 MI Chengrong1
(1. Department of Ultrasound, the General Hospital of Ningxia Medical University, Yinchuan 750004; 2. Department of Anal-Colorectal Surgery, the General Hospital of Ningxia Medical University, Yinchuan 750004)
关键词:
超声联合微泡造影技术BOP1基因肝纤维化基因治疗大鼠
Keywords:
microbubble contrast agent combined ultrasound techniquesblock of proliferation1liver fibrosisgene therapyrat
分类号:
R445.1
DOI:
10.16050/j.cnki.issn1674-6309.2018.06.005
文献标志码:
A
摘要:
目的 探讨超声辐照携载短发夹RNA干扰增殖1阻断(block of proliferation1,BOP1)基因的超声微泡造影剂治疗大鼠肝纤维化的可行性。方法 成功构建大鼠肝纤维化模型,将40只肝纤维化大鼠随机分为携载质粒的超声微泡组(BOP1-shRNA+US/MB)、携载质粒的常规超声组(BOP1-shRNA+US)、单纯质粒组 (BOP1-shRNA)及空白对照组(CON),每组10只。BOP1-shRNA+US/MB组及BOP1-shRNA+US组分别经股静脉注入1mL微泡溶液及1mL携载单纯质粒的生理盐水后,行肝区超声辐照。所有分组大鼠在干预21d后行磁共振扩散加权成像检查后麻醉脱颈处死,取肝脏组织行苏木精-伊红(HE)染色观察病理变化。应用Western blot法检测BOP1蛋白在大鼠肝脏组织中的表达,检验BOP1的沉默效果。结果 BOP1-shRNA+US/MB组的表观弥散系数(ADC)值高于其他3组,而指数表观弥散系数(EADC)值低于其他3个组(P<0.05)。HE染色示BOP1-shRNA+US/MB组肝脏细胞排列尚整齐,汇管区纤维组织增生,见少量炎性细胞浸润,小叶结构完整,其肝纤维化程度低于其他组。Western blot结果显示BOP1-shRNA+US/MB组大鼠肝脏组织的BOP1蛋白表达敲减成功。结论 超声辐照携载BOP1基因短发夹RNA的微泡造影剂可成功治疗大鼠肝纤维化,可为肝纤维化的精准靶向治疗提供新的理论依据。
Abstract:
Objective To evaluate the feasibility of employing ultrasound-targeted microbubble destruction which mediated BOP1 gene silencing to treat rat live fibrosis. Methods Rat models of liver fibrosis were successfully constructed. Rats were divided into four groups:BOP1-shRNA+US/MB,BOP1-shRNA+US, simple plasmid group(BOP1-shRNA) and blank control group(CON),with 10 rats in each group. Group BOP1-shRNA+US/MB and BOP1-shRNA+US were injected with 1mL microbubble solution via femoral vein and 1mL normal saline with plasmid. After intervention for 21d, all rats were sacrificed by magnetic resonance diffusion weighted imaging(DWI),and liver tissue was stained with Hematoxylin Eosin(HE). Western blot was used to detect the expression of BOP1 protein in rat liver, and to test the silencing effect of BOP1. Results The apparent diffusion coefficient (ADC) of group BOP1-shRNA+US/MB was higher than that of the other 3 groups, while the index apparent diffusion coefficient(EADC) value was lower than that of the other 3 groups(P<0.05). HE staining showed that the liver cells in group BOP1-shRNA+US/MB were neatly arranged, fibrous tissue proliferated in the pipe area,a small amount of inflammatory cells were infiltrated,and the structure of the lobule was complete, and the degree of liver fibrosis was lower than that of the other groups. Western blot results showed that the expression of BOP1 protein in liver tissue of BOP1-shRNA+US/MB group was knocked down successfully. Conclusion The microbubble contrast agent carrying BOP1 gene short hairpin RNA can successfully treat the liver fibrosis in rats. It can provide a new theoretical basis for the precise targeting therapy of liver fibrosis.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期:2017-11-29
基金项目:国家自然科学基金(81560394);宁夏自然科学基金(NZ15161)
作者简介:马丽园(1986-),女,硕士,主治医师,主要从事超声医学研究。E-mail: mly8699@163.com
通信作者:米成嵘(1963-),男,教授,主任医师,硕士研究生导师,从事腹部超声、介入超声及超声造影等研究。 E-mail:12214274@qq.com
更新日期/Last Update: 2018-06-30