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[1]尹译惠,刘 云,刘炳霞,等.Napsin A、p40在肺腺癌、鳞癌中的表达及其与能谱CT定量指标的相关性[J].宁夏医科大学学报,2018,(04):429-433,438.[doi:10.16050/j.cnki.issn1674-6309.2018.04.012]
 YIN Yihui,LIU Yun,LIU Bingxia,et al.Correlation between Expression of Napsin A and p40 in Lung Adenocarcinoma and Squamous Cell Carcinoma with QuantitativeAnalysis of CT by Energy Spectral[J].Ningxia Medical University,2018,(04):429-433,438.[doi:10.16050/j.cnki.issn1674-6309.2018.04.012]
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Napsin A、p40在肺腺癌、鳞癌中的表达及其与能谱CT定量指标的相关性(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2018年04期
页码:
429-433,438
栏目:
论 著
出版日期:
2019-09-30

文章信息/Info

Title:
Correlation between Expression of Napsin A and p40 in Lung Adenocarcinoma and Squamous Cell Carcinoma with Quantitative Analysis of CT by Energy Spectral
作者:
尹译惠1 刘 云2 刘炳霞1 王艺霖1 李 妮1 王 楠1 任小璐2
(1. 宁夏医科大学,银川 750004; 2. 宁夏医科大学总医院心脑血管病医院放射科,银川 750002)
Author(s):
YIN Yihui1 LIU Yun2 LIU Bingxia1 WANG Yilin1 LI Ni1 WANG Nan1 REN Xiaolu2
(1. Ningxia Medical University,Yinchuan 750004; 2. Department of Radiology, the General Hospital of Ningxia Medical University,Cardio Cerebrovascular Disease Hospital, Yinchuan 750002)
关键词:
肺癌体层摄影术X线计算机扫描Napsin Ap40
Keywords:
lung cancer tomography X-ray computed Napsin A p40
分类号:
R734.2;R814.42
DOI:
10.16050/j.cnki.issn1674-6309.2018.04.012
文献标志码:
A
摘要:
目的 探讨肺腺癌及鳞癌中Napsin A、p40表达及其与能谱CT 参数的相关性。方法 选取我院2016年1月至2017年4月期间行能谱CT扫描并经病理确诊的非小细胞肺癌患者68例(腺癌组36例,鳞癌组32例)。利用能谱成像对两组病例进行多参数定量分析,测得其平扫病变的有效原子序数(Effective-z)、钙含量、增强病变的标准碘含量(NIC)、70keV单能量下肿块的强化程度ΔCT及能谱曲线斜率(k),采用免疫组化PV二步法测定腺癌组及鳞癌组中NapsinA、p40的表达情况。结果 肺腺癌组与肺鳞癌组的Effective-z、钙含量、NIC以及能谱曲线斜率差异均有统计学意义(P均<0.05),病灶在70keV单能量下的强化程度两组间差异无统计学意义(P>0.05)。Napsin A、p40在肺腺癌与肺鳞癌中的表达差异有统计学意义(P<0.05)。Napsin A在肺腺癌组的表达阳性率为97.22%,其表达情况与能谱CT的Effective-z、钙含量、NIC、能谱曲线斜率均呈正相关(P均<0.05);p40在肺鳞癌组的表达阳性率为100%,其表达情况与能谱CT的Effective-z、NIC、能谱曲线斜率均呈正相关(P均<0.05);肺鳞癌组p40的表达与钙含量呈负相关(P<0.05)。结论 能谱CT成像的多参数定量分析对术前肺腺癌与鳞癌的鉴别诊断具有重要价值,对预测肿瘤某些生物学行为方面具有潜在价值。
Abstract:
Objective To investigate the relationship between the Napsin A and p40 expression in lung adenocarcinoma and squamous carcinoma with its spectral CT parameters. Methods A total of 68 patients with non-small cell lung cancer diagnosed by spectral CT and pathology were selected from our hospital between January 2016 and April 2017 as the study subjects(adenocarcinoma Group 36 cases,squamous carcinoma Group 32 cases). Spectral CT imaging quantitative parameter analysis were used on two groups of cases. The Effective atomic number(Effective-z) and calcium content of the lesions by CT plain scan、Normalized iodine concentrations(NIC)on contrast enhanced CT、enhancement degree of the masses under 70keV monochromatic spectral CT(ΔCT)and spectral curve slope were measured by the multiparameter quantitative analysis of two groups of cases. Immunohistochemistry of PV two-step staining was performed to detect the expression of Napsin A and p40 in adenocarcinoma group and squamous carcinoma group. Results Effective-z,calcium content,NIC and the slope of energy spectrum in the lung adenocarcinoma group and the lung squamous cell carcinoma group were statistically significant(P<0.05). There was no significant difference between the two groups(P>0.05) for the intensification of the foci under the single 70keV energy. The expression of Napsin A and p40 in lung adenocarcinoma and lung squamous cell carcinoma was significantly different(P<0.05). The positive rate of Napsin A in lung adenocarcinoma was 97.22%, and its expression was positively correlated with Effective-z,calcium content,NIC and the slope of energy spectrum of CT(P<0.05). The positive rate of p40 in lung squamous cell carcinoma was 100%,and its expression was positively correlatedwith Effective-z, NIC and the slope of the energy spectrum curve (P<0.05). The expression of P40 in the lung squamous cell carcinoma group was negatively correlated with the calcium content(P<0.05). Conclusion Multiparameter quantitative of spectral CT imaging is of great value in the diagnosis and differential diagnosis of preoperative lung adenocarcinoma and squamous carcinoma, and has potential value in predicting some biological behaviors of tumors.

参考文献/References:


[1] Siegel RL,Miller KD,Fedewa SA. Colorectal Cancer Statistics,2017[J]. CA: A Cancer Journal for Clinicians,2017,67(3):177-193.
[2] Chen W,Zheng R,Baade PD,et al. Cancer statistics in China,2015[J]. CA: A Cancer Journal for Clinicians, 2016,66(2):115-132.
[3] Travis WD,Brambilla E,Noguchi M,et al. International association for the study of lung Cancer/American thoracic Society/European respiratory society international multidisciplinary classification of lung adenocarcinoma[J]. Journal of Thoracic Oncology,2011,6(2):244-285.
[4] Whithaus K,Fukuoka J,Prihoda TJ,et al. Evaluation of napsin A,cytokeratin 5/6,p63,and thyroid transcription factor 1 in adenocarcinoma versus squamous cell carcinoma of the lung[J]. Arch Pathol Lab Med,2012,136(2):155-162.
[5] Mukhopadhyay S,Katzenstein AL. Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1,napsin A,p63,and CK5/6[J]. Am J Surg Pathol,2011,35(1):15-25.
[6] Yang F,Dong J,Wang X,et al. Non-small cell lung cancer: Spectral computed tomography quantitative parameters for preoperative diagnosis of metastatic lymph nodes[J]. European Journal of Radiology, 2017,89:129-135.
[7] Furuya K,Yasumori K,Takeo S,et al. Lung CT: Part 1,mimickers of lung cancer-spectrum of CT findings with pathologic correlation[J]. American Journal of Roentgenology,2012,199(4):W454-W463.
[8] Kim MJ,Shin HC,Shin KC,et al. Best immunohistochemical panel in distinguishing adenocarcinoma from squamous cell carcinoma of lung: tissue microarray assay in resected lung cancer specimens[J]. Annals of Diagnostic Pathology,2013,17(1):85-90.
[9] 刘标,周晓军. 非小细胞肺癌免疫组化标志物专家共识(2014)[J]. 临床与实验病理学杂志,2015,31(5):481-487.
[10] 张文,于长海,李英杰,等. 肺癌CT值与肿瘤分化程度、病理类型及淋巴结转移相关性的临床研究[J]. 临床肺科杂志,2011,16(10):1554-1555.
[11] Khanduri S,Bhagat S,Shokeen P,et al. Rationale of using dynamic imaging for characterization of suspicious lung masses into benign or malignant on contrast enhanced eulti detector computed tomography[J]. J Clin Imaging Sci,2017,7(24):1-8.
[12] 钱利明. 宝石能谱CT成像的临床应用[J]. 实用医学影像杂志,2015,16(1):82-83.
[13] Wang G,Zhang C,Li M,et al. Preliminary application of high-definition computed tomographic gemstone spectral imaging in lung cancer[J]. Journal of Computer Assisted Tomography,2014,38(1):77-81.
[14] 欧阳爱梅,苏新友,魏兆龙. 宝石能谱CT成像在肿瘤诊疗中的应用进展[J]. 中国临床医学影像杂志,2015,26(8):602-604.
[15] 张武. 宝石能谱CT成像在不同组织来源、病理类型肿瘤及其转移淋巴结中的诊断价值[J]. 临床和实验医学杂志,2016,15(8):813-815.
[16] Bradley MT,Philip TC. A new marker for lung adenocarcinoma, is complementary and more sensitive and specific than thyroid transcription factor 1 in the differential diagnosis of primary pulmonary carcinoma evaluation of 1674 cases by tissue microany[J]. Arch Pathol Lab Med,2012,136(2):163-171.
[17] Chernock RD,El-Mofty SK,Becker N,et al. Napsin A expression in anaplastic, poorly differentiated,and micropapillary pattern thyroid carcinomas[J]. Am J Surg Pathol,2013,37(8):1215-1222.
[18] Nishino M,Hoang MP,Della Pelle P,et al. Napsin A/p40 antibody cocktail for subtyping non-small cell lung carcinoma on cytology and small biopsy specimens[J]. Cancer Cytopathol,2016,124(7):472-484.
[19] Ezzat Nel-S,Tahoun N. The role of Napsin-A and Desmocollin-3 in classifying poorly differentiating non-small cell lung carcinoma[J]. Journal of the Egyptian National Cancer Institute,2016,28(1):13-22.
[20] Yokohira M,Nakano-Narusawa Y,Yamakawa K,et al. Validating the use of napsin A as a marker for identifying tumorigenic potential of lung bronchiolo-alveolar hyperplasia in rodents[J]. Experimental and Toxicologic Pathology:Official Journal of the Gesell-schaft fur Toxikologische Pathologie,2017,69(8):637-642.
[21] Beck J,Miller MA,Frank C,et al. Surfactant protein A and napsin A in the Immunohistochemical characterization of canine pulmonary carcinomas: comparison with thyroid transcription factor-1[J]. Veterinary Pathology,2017,54(5):767-774.
[22] Pilji■ Burazer M, Mladinov S,■apkun V,et al. The utility of thyroid transcription Factor 1(TTF-1), napsin A,excision repair cross-complementing 1 (ERCC1),anaplastic lymphoma linase(ALK) and the epidermal growth factor receptor(EGFR) expression in small biopsy in prognosis of patients with lung adenocarcinoma-A retrograde single-center study from croatia[J]. Medical Science Monitor,2017,23:489-497.
[23] 章文华,项晶晶,孙丽娟,等. 肺腺癌中TTF-1与Napsin A的病理诊断价值比较[J]. 临床与实验病理学杂志,2016,32(9):1028-1032.
[24] Righi L,Graziano P,Fornari A,et al. Immunohistochemical subtyping of nonsmall cell lung cancer not otherwise specified in fine-needle aspiration cytology: a retrospective study of 103 cases with surgical correlation[J]. Cancer,2011,117(15):3416-3423.
[25] Nobre AR,Albergaria A,Schmitt F. p40:a p63 isoform useful for lung cancer diagnosis-a review of the physiological and pathological role of p63[J]. Acta Cytologica,2013,57(1):1-8.
[26] Bishop JA,Teruya-Feldstein J,Westra WH,et al. p40(ΔNp63) is superior to p63 for the diagnosis of pulmonary squamous cell carcinoma[J]. Modern Pathology,2012,25(3):405-415.
[27] Takahiro T,Koji T,Kyohei M,et al. p40 is the best marker for diagnosing pulmonary squamous cell Carcinoma: comparison with p63,cytokeratin 5/6,desmocollin-3, and Sox2[J]. Immunohistochemistry & Molecular Morphology:AIMM,2014,22(5):377-82.
[28] 仇加高. p40、天门冬氨酸蛋白酶、甲状腺转录因子-1在肺鳞癌与腺癌表达敏感性和特异性[J]. 交通医学,2014,28(4):334-336.

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备注/Memo

备注/Memo:
收稿日期:2017-09-28
作者简介: 尹译惠(1992-),女,在读硕士研究生,从事影像医学与核医学专业。
通信作者: 刘云,主任医师,主要研究胸部影像学。 E-mail: yunliusky@163.com
更新日期/Last Update: 2018-04-30