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[1]杨芝红,白永泽,王贵杰.FCM免疫表型分析在急性早幼粒细胞白血病MICM分型诊断中的意义[J].宁夏医科大学学报,2017,(11):1272-1276.[doi:10.16050/j.cnki.issn1674-6309.2017.11.008]
 YANG Zhihong,BAI Yongze,WANG Guijie.Clinical Significance of FCM Immunophonotype Analysis on MICM Classification Diagnosis in Patients with Acute Promyelocytic Leukemia[J].Ningxia Medical University,2017,(11):1272-1276.[doi:10.16050/j.cnki.issn1674-6309.2017.11.008]
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FCM免疫表型分析在急性早幼粒细胞白血病MICM分型诊断中的意义(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2017年11期
页码:
1272-1276
栏目:
论著
出版日期:
2017-11-30

文章信息/Info

Title:
Clinical Significance of FCM Immunophonotype Analysis on MICM Classification Diagnosis in Patients with Acute Promyelocytic Leukemia
作者:
杨芝红白永泽王贵杰
宁夏医科大学总医院医学实验中心,银川 750004
Author(s):
YANG ZhihongBAI YongzeWANG Guijie
Medical laboratory Center of General Hospital,Ningxia Medical University,Yinchuan 750004
关键词:
流式细胞术急性髓系白血病免疫表型
Keywords:
flow cytometryacute promyelocytic leukemia immunophenotype
分类号:
R733.71
DOI:
10.16050/j.cnki.issn1674-6309.2017.11.008
文献标志码:
A
摘要:
目的 探讨急性早幼粒细胞白血病流式细胞术(FCM)免疫表型分析及其在MICM分型诊断中的临床意义。方 法 FCM免疫表型分析采用流式细胞术多参数荧光标记抗体检测方法,对63例临床最终诊断为急性早幼粒细胞白血 病(APL)患者的骨髓形态、免疫分型、遗传学、分子生物学等检测结果进行综合分析。结果 63例APL其FAB分析 结果,56例为M3a型,6例为M3b型,1例为AML不除外M3;免疫表型分析结果显示CD33、CD13、CD117、MPO表达阳性 率分别为100%、95.2%、92.1%和96.8%,大多数APL不表达CD34、HLA-DR,CD15、CD11b的表达均为部分或极少部分 细胞的弱表达。染色体核型分析结果显示3例为正常核型、2例未见分裂相、1例因可分析细胞数极少而无法分析、 57例可见特异性t(15;17)异常,其中有11例核型分析除可见t(15;17)异常外7例还伴有额外异常、4例伴有 其它复杂异常。分子生物学检测结果显示63例均可见PML/RARa融合基因阳性。结论 在APL的MICM分型诊断中,FCM 免疫表型分析虽然不是诊断APL的独立指标,但可以为APL的快速鉴别与诊断提供依据。
Abstract:
Objective To investigate the clinical significance of FCM immunophonotype analysis on MICM classification diagnosis in patients with acute promyelocytic leukemia. Methods FCM immunophonotye analysis was performed with multiparameter fluorescence labeling method. Marrow morphology,immunophonotye,genetic testing results and molecular biology results were analyzed synthetically in 63 patients with APL immunophenotype. Results FAB results in 63 cases with APL showed that 56 cases were M3a,6 cases were M3b and 1 case with AML. Immunophonotype analysis showed that the expression positive rates of CD33,CD13,CD117,MPO were 100%,95.2%,92.1% and 96.8%,respectively,and most APL patients did not express CD34,HLA-DR while CD15 and CD11b showed weak expression in some cells. Karyotype analysis showed that normal in 3 cases,no split phase in 2 cases,specific t(15;17) abnormity in 57 cases,(15;17) abnormity with other abnormities in 11 cases,other complicated abnormitiesin 4cases. Molecular biological analysis showed PML / RARa fusion gene positive in 63 cases. Conclusion In MICM classification diagnosis of APL,morrow morphology is important but the FCM is the necessary supplementary method. Though FCM immunophonotype is not the independent index,it may provide the fast and accurate base for diagnosing the APL joint detection of FCM immunophonotype and MICM classification can improve the detection rate and accuracy.

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备注/Memo

备注/Memo:
收稿日期:2017-07-06
作者简介: 杨芝红,女,硕士,主任技师,主要从事流式细胞技术工作。
更新日期/Last Update: 1900-01-01