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[1]王萌,高小茹,张冬梅,等.JNK对舒芬太尼预处理缺氧/复氧损伤心肌细胞Cx43表达的影响[J].宁夏医科大学学报,2017,(02):131-134,139.[doi:10.16050/j.cnki.issn1674-6309.2017.02.004]
 WANG Meng,GAO Xiaoru,ZHANG Dongmei,et al.Effect of JNK on Connexin 43 Expression by Sufentanil Preconditioning in Rat Myocardial Cells with Hypoxia / Reoxygenation Injury[J].Ningxia Medical University,2017,(02):131-134,139.[doi:10.16050/j.cnki.issn1674-6309.2017.02.004]
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JNK对舒芬太尼预处理缺氧/复氧损伤心肌细胞Cx43表达的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2017年02期
页码:
131-134,139
栏目:
论著
出版日期:
2017-02-28

文章信息/Info

Title:
Effect of JNK on Connexin 43 Expression by Sufentanil Preconditioning in Rat Myocardial Cells with Hypoxia / Reoxygenation Injury
作者:
王萌12高小茹12张冬梅3王建珍3郑月梅3江海峰4
1.宁夏医科大学,银川 750004; 2.宁夏颅脑疾病重点实验室,银川 750004; 3.宁夏医科大学总医院麻醉科,银川 750004; 4.宁夏医科大学总医院病理科,银川 750004
Author(s):
WANG Meng12 GAO Xiaoru12 ZHANG Dongmei3 WANG Jianzhen3ZHENG Yuemei3 JIANG Haifeng4
1. Ningxia Medical University, Yinchuan 750004; 2. Ningxia Key Laboratory of Cerebrocranial Diseases ,Yinchuan 750004; 3. Department of Anesthesiology, the General Hospital of Ningxia Medical University, Yinchuan 750004; 4. Department of Pathology,the Gen
关键词:
舒芬太尼缺氧/复氧损伤缝隙连接蛋白43JNK信号通路
Keywords:
sufentanil hypoxia /reoxygenation injuryconnexin 43JNK signaling pathway
分类号:
R614
DOI:
10.16050/j.cnki.issn1674-6309.2017.02.004
文献标志码:
A
摘要:
目的 探讨舒芬太尼预处理对缺氧/复氧乳鼠心肌细胞缝隙连接蛋白43(Connexin 43 Cx43)表达的影响及JNK(c-Jun N-terminal kinases)蛋白激酶信号通路的作用。方法 原代SD大鼠心肌细胞培养5d后,检测细胞纯度;使用95%N2+5%CO2混合气体饱和的缺氧液,无氧环境下孵育2h后正常培养1h以建立缺氧/复氧模型,并检测细胞活力;随机分为7组:对照组(C组)、缺氧/复氧组(H/R组)、吗啡预处理组(M组)、舒芬太尼预处理组(S组)、JNK抑制剂组(IJ组)、JNK抑制剂+吗啡预处理组(MJB组)、JNK抑制剂+舒芬太尼预处理组(SJB组)。缺氧前30min,各处理组分别按相应分组给予吗啡(0.3μmol·L-1)、舒芬太尼(0.3×10-3μmol·L-1)、JNK抑制剂(SP600125,20μmol·L-1)、SP600125+吗啡(20μmol·L-1+0.3μmol·L-1)、SP600125+舒芬太尼(20μmol·L-1+0.3×10-3μmol·L-1)处理,激光共聚焦显微镜观察各组Cx43的变化并测定平均荧光密度;Western blot检测各组Cx43蛋白及Ser368位点的磷酸化蛋白的表达。结果 与C组比较,其余各组p-Cx43Ser368及Cx43蛋白表达降低(P<0.05);与H/R组比较,M组、S组、MJB组、SJB组p-Cx43Ser368及Cx43蛋白表达升高(P<0.05),IJ组差异无统计学意义(P>0.05);与M组比较,IJ组p-Cx43Ser368及Cx43蛋白表达降低(P<0.05),S、MJB组表达差异无统计学意义(P>0.05);与S组比较,IJ组p-Cx43Ser368及Cx43蛋白表达降低(P<0.05),SJB组表达差异无统计学意义(P>0.05)。结论 缺氧/复氧损伤条件下,舒芬太尼预处理可以产生与吗啡类似的稳定心肌Cx43的保护作用,这种作用可能与JNK信号通路有关。
Abstract:
Objective To explore the expression of Connexin 43(Cx43) in rat myocardial cells with hypoxia/ reoxygenation injury by sufentanil preconditioning and the effect of JNK on signaling pathway. Methods Primary SD rat myocardial cells were cultured for 5 days and cell purity was detectedThe cells were exposed to hypoxia solution saturated with 95% N2+5% CO2 for 2h,and cultured in the normal atmosphere for 1h. Hypoxia/reoxygenation model was established and,cell viability was detected. Myocardial cells randomly divided into 7 groups: control group (group C), hypoxia / reoxygenation group (H/R group), morphine preconditioning group (group M), sufentanil preconditioning group (group S), JNK inhibitor group (group IJ), JNK inhibitor + morphine preconditioning group(group MJB), JNK inhibitor + sufentanil preconditioning group (group SJB). Before hypoxia 30min,each group which treatment with relevant group was given morphine(0.3?滋mol·L-1), sufentanil(0.3×10-3?滋mol·L-1),JNK inhibitor(SP600125,20?滋mol·L-1), SP600125+morphine(20?滋mol·L-1+ 0.3?滋mol·L-1),SP600125+sufentanil(20?滋mol·L-1+0.3×10-3?滋mol·L-1). Cx43 expression and average density in each group were measured by confocal microscopy. The Cx43 expression and Cx43-Ser368 sites phosphorylation were detected by Western blot. Results Compared with group C,p-Cx43Ser368, Cx43 protein expression of the rest groups were reduced significantly(P<0.05). Compared with group H/R,p-Cx43-Ser368,Cx43 protein expression were significantly increased in group M,group S,group MJB,group SJB(P<0.05). Compared with group M,p-Cx43Ser368, Cx43 protein expression were decreased significantly in group IJ(P<0.05). Compared with group S,p-Cx43Ser368,Cx43 protein expression was decreased(P<0.05) in group IJ. Conclusion Sufentanil preconditioning can produce similar protective effect about Cx43 with morphine on hypoxia / reoxygenation injury myocardial cells and enhance the level of Cx43-Ser368 sites phosphorylation.This effect might be related to JNK signal pathway.

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备注/Memo

备注/Memo:
收稿日期:2016-08-06 基金项目:国家自然科学基金(81260029) 作者简介: 王萌,男,在读硕士研究生,研究方向:围手术期心肌保护。 通信作者:张冬梅,女,教授,硕士研究生导师。E-mail:zdm_ych@hotmail.com
更新日期/Last Update: 2017-02-20