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[1]霍国进,邹有瑞,夏明,等.枸杞多糖对脑出血模型大鼠继发性脑损伤的影响[J].宁夏医科大学学报,2016,(02):125-128,147.[doi:10.16050/j.cnki.issn1674-6309.2016.02.003]
 HUO Guojin,ZOU Yourui,XIA Ming,et al.The Effect of Lycium Barbarum Polysaccharides on Secondary Brain Injury in Intracerebral Hemorrhage Rats[J].Ningxia Medical University,2016,(02):125-128,147.[doi:10.16050/j.cnki.issn1674-6309.2016.02.003]
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2016年02期
页码:
125-128,147
栏目:
论著
出版日期:
2016-02-20

文章信息/Info

Title:
The Effect of Lycium Barbarum Polysaccharides on Secondary Brain Injury in Intracerebral Hemorrhage Rats
作者:
霍国进1邹有瑞2夏明1蒋树财3王军成1沈冰2
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院神经外科,银川 750004; 3.山东滨州市人民医院神经外科,滨州 256610
Author(s):
HUO Guojin1 ZOU Yourui2 XIA Ming1 JIANG Shucai3 WANG Juncheng1 SHEN Bing2
1.Ningxia Medical University, Yinchuan 750004; 2.Dept. of Neurosurgery, the General Hospital of Ningxia Medical University, Yinchuan 750004; 3.Dept. of Neurosurgery, Binzhou People's Hospital, Binzhou 256610
关键词:
枸杞多糖 脑出血 继发性脑损伤 保护作用 大鼠
Keywords:
lycium barbarum polysaccharides intracerebral hemorrhage secondary brain injury protective effect rats
分类号:
R285 651.1+5
DOI:
10.16050/j.cnki.issn1674-6309.2016.02.003
文献标志码:
A
摘要:
目的 探讨枸杞多糖(lycium barbarum bolysaccharides,LBP)对脑出血(intracerebral hemorrhage,ICH)模型大鼠继发性脑损伤的影响。方法 SD大鼠90只,随机分为假手术组、模型组、LBP低剂量组、LBP中剂量组、LBP高剂量组、尼莫地平组,每组15只; 术前15日,LBP低剂量、LBP中剂量、LBP高剂量组分别给予LBP 50、100、200mg·kg-1灌胃,尼莫地平组给予尼莫地平10mg·kg-1灌胃,假手术组和模型组给予等量的生理盐水,每日1次; 灌胃结束后,采用自体血尾状核注射法制作大鼠脑出血模型; 在脑出血后4、8、12、24和48h分别对各组大鼠进行神经功能评分; 评分结束后断头取脑,检测各组大鼠脑组织水肿体积、脑含水量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量; HE染色后观察脑出血区及其周围脑组织的神经细胞、胶质细胞等的大小、形态。结果 与假手术组比较,其他各组大鼠神经功能评分较高、脑组织水肿体积较大、脑含水量较高、SOD活性较低、MDA含量较多(P<0.05 或 P<0.01)。与模型组比较,LBP各组大鼠神经功能评分仅部分时点减低(P<0.05),脑组织水肿体积减小、脑含水量降低、SOD活性增高、MDA含量降低(P<0.05或P<0.01)。假手术组HE染色结果未见明显异常,模型组与各给药组HE染色结果均显示脑出血区均有血凝块形成,出血区周围脑组织有不同程度的破坏,并可见排列紊乱的神经细胞与神经胶质细胞。结论 预防性应用枸杞多糖能够减轻脑出血后继发性脑损伤,对脑出血后脑组织起到一定的保护作用,对预防脑出血有积极的意义。
Abstract:
Objective To explore the effect of Lycium Barbarum Polysaccharides(LBP)on the secondary brain injury in rat models of Intracerebral hemorrhage(ICH). Methods 90 SD rats were randomly divided into sham group, model group, LBP low dose group,LBP moderate dose group, LBP high dose group and nimodipine group. Preoperatively 15th day, rats of LBP low dose group, LBP moderate dose group, LBP high dose group, was given LBP 50, 100, 200 mg·kg-1 gavage, respectively while rats of nimodipine group was given nimodipine 40mg·kg-1 gavage and rats in sham group and model group were given the same amount of saline, 1 time / day. After gavage,rat ICH model was established using autologous blood injection caudate nucleus. The neurological function score grading of rats was carried out. the brain edema volume, brain water content,SOD activity and MDA content were detected in brain tissue of rats. the cell size and morphology werebserved after HE staining. Results Compared with sham group, the neurological function score of rats was higher, brain edema volume was larger, brain water content was taller, SOD activity was lower and MDA content was higher in other groups(P<0.05 or P<0.01). Compared with model group, neurological function scores at part timepoint in LBP groups were reduced(P<0.05), brain edema volume was alleviated, brain water content was reduced, SOD activity increased and MDA content decreased significantly(P<0.05 or P<0.01). The HE staining of sham group showed no significant abnormalities, but the HE staining in other groups showed: blood clot formed in the ICH region, brain tissue around the ICH region had different degree of damage, and nerve cells and gliocyte cells disordered arrangement. Conclusion Prophylactic use of LBP can reduce the secondary brain injury of ICH. It has a protective effect on ICH and has a positive significance in prevention against ICH.

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备注/Memo

备注/Memo:
收稿日期:2015-12-01 作者简介:霍国进(1989-),男,在读硕士研究生。 通信作者:沈冰,主任医师,博士,研究生导师,从事神经外科疾病基础及临床研究。E-mail:shenbing315@sina.com
更新日期/Last Update: 2016-02-20