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[1]王艳,郑晓敏,张晓春.儿童急性白血病CD34、CD117抗原表达分析[J].宁夏医科大学学报,2014,(06):641-645.
 WANG Yan,ZHENG Xiaomin,ZHANG Xiaochun.The Clinical Significance of CD34 and CD117 Antigen Expression in Pediatric Acute Leukemia[J].Ningxia Medical University,2014,(06):641-645.
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儿童急性白血病CD34、CD117抗原表达分析(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2014年06期
页码:
641-645
栏目:
论著
出版日期:
2014-06-30

文章信息/Info

Title:
The Clinical Significance of CD34 and CD117 Antigen Expression in Pediatric Acute Leukemia
作者:
王艳1郑晓敏2张晓春2
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院儿科,银川 750004
Author(s):
WANG Yan1ZHENG Xiaomin2ZHANG Xiaochun2
WANG Yan1, ZHENG Xiaomin2, ZHANG Xiaochun2
关键词:
急性白血病CD34CD117儿童
Keywords:
acute leukemia CD34 CD117 children
分类号:
R725.5
DOI:
-
文献标志码:
A
摘要:
目的探讨儿童急性白血病(AL)CD34和CD117抗原表达的临床意义。方法采用流式细胞术对68例急性淋巴细胞白血病(ALL)及43例急性髓系白血病(AML)患儿的骨髓细胞进行CD34和CD117抗原检测,并比较CD34、CD117和CD34/CD117共表达在AL患儿表达的差异及与诱导化疗疗效的关系。结果CD34在ALL和AML患儿中均有表达,差异无统计学意义(P>0.05)。而CD117、CD34/CD117在AML患儿中的表达率明显高于ALL患儿(P<0.05)。AL患儿中CD34+组肝脾、淋巴结肿大及首轮诱导化疗缓解(CR)率与CD34-组差异均有统计学意义(P<0.05);CD34+/CD117+组CR率明显低于CD34-/CD117-组(P<0.05)。ALL中回族患儿CD117表达率高于汉族患儿(χ2=43.391,P=0.000)。CD117+及CD34+/CD117+组血小板(PLT)水平分别较阴性组低(P<0.05)。在AML患儿中CD34+组PLT水平较CD34-组低(P<0.05),CD117+、CD34+/CD117+组肝脾肿大分别较阴性组多见(P<0.05)。AML患儿中CD34、CD117、CD34/CD117在汉族阳性表达率均高于回族(P<0.05)。结论CD34抗原表达是儿童AL的独立高危因素,临床特征上主要表现为髓外浸润。CD117、CD34/CD117可作为AL患者MICM诊断分型的髓系免疫学依据。CD34/CD117有助于AML的诊断。CD34、CD34/CD117的表达可作为预测AL诱导化疗疗效的指标。
Abstract:
Objective To analyze the clinical significance of CD34 and CD117 antigen expression in acute leukemia(AL)patients.Methods Immune phenotype of bone marrow cell were detected in 68 cases with acute lymphoblastic leukemia(ALL)and 43 cases with acute myeloid leukemia(AML)patients by flow cytometry. The difference of CD34, CD117, CD34/CD117 expression and their correlation with chemotherapy response was compared in AL patients. Results CD34 were expressed in both ALL and AML patients and there was no statistically significant difference(P>0.05). The expression of CD117 and CD34/CD117 in AML patients were significantly higher than that in ALL patients(P<0.05). Among AL patients, significant differences(P<0.05)were found in Hepatosplenomegaly, lymphadenectasis and complete remission(CR)rate after induction therapy between CD34+ and CD34-group. The CR rate was significantly lower in CD34+/CD117+ group than that in CD34-/CD117-group. Among ALL patients, the expression of CD117 was higher in Hui ethnic group than Han ethnic group(χ2=43.391, P=0.000). Platelet count was lower in CD117+ and CD34+/CD117+ group than CD117- and CD34-/CD117-group(P<0.05). Among AML patients, platelet count was lower in CD34+ group than CD34-group(P<0.05)Significant differences was also found when compared the hepatosplenomegaly between CD117+,CD34+/CD117+ and CD11-,CD34-/CD117- group(P<0.05). The expression of CD34,CD117,CD34/CD117 was higher in Han ethnic group than Hui ethnic group(P<0.05). Conclusion The expression of CD34 antigen is one of independent of the risk factors and highly correlated with extramedullary infiltration in AL patients. The co-expression of CD34/CD117 is helpful in the diagnosis of AML. The co-expression rate of CD117/CD34 and CD117 may be an immunologic marker for AML in MICM. The expression of CD34 and CD34/CD117 of patients can be used as the index to predict chemotherapy response in AL.

参考文献/References:

[1] 沈红强,汤永民,杨世隆,等. 多色流式细胞术在222例儿童急性白血病免疫分型中的应用[J].中华儿科杂志,2003,41(5):334-337.
[2] 张之南.血液病诊断及疗效标准[M].2版.北京:科学技术出版社,1998:184-214.
[3] 邱阳,王刚,刘越坚,等.三色流式细胞术分析急性淋巴细胞白血病免疫分型[J].中国实验血液学杂志,2009,17(2):442-444.
[4] 李熙鸿,熊珍玉,王晓阳.儿童急性淋巴细胞白血病CD34+表达的临床意义[J].中华妇幼临床医学杂志,2006,2(3):140-142.
[5] 谢建兴,罗旭华,郭秋红,等. 急性髓系白血病中CD117的表达与临床意义[J].中国医药科学,2012,2(18):41-42.
[6] 刘艳荣,付家瑜,常艳,等. CD117在白血病免疫分型中的意义[J].中华检验医学杂志,2000,23(4):211-213.
[7] Sazawal S,Bakhshi S,Raina V,et al.Detection and clinical relevance of BCR-ABL fusion gene in childhood T-lineage acute lymphoblastic leukemia:a report on 4 cases[J].J Pediatr Hematol Oncol,2009,31(11):850-852.
[8] Shman U,Savitski VP.CD34+leukemic subpopulation predominantly displays lower spontaneous apoptosis and has higher expression levels of Bcl-2 and MDR1 genes than CD34 cells in childhood AML[J].Ann Hematol,2008,87:353-360.
[9] 黄婕,何璐璐,方拥军.儿童急性白血病免疫表型及临床研究[J]. 医学临床研究,2008,25(12):2159-2162.
[10] Auewarakul CU,Lauhakirti D,Promsuwicha O,et al. C-kit receptor tyrosine kinase(CD117)expression and its positive predictive value for the diagnosis of Thai adult acute myeloid leukemia. Ann Hematol. 2006 Feb; 85(2):108-112.
[11] 谢建兴,谭禾英,彭敏,等.急性白血病患者CD117与CD34的表达及临床意义[J].中国现代医生,2012,50(25):58-59.
[12] Tavil B,Cetin M,Tuncer M.CD34/CD117 positivity in assessment of prognosis in children with myelodysplastic syndrome. Leuk Res. 2006 Feb; 30(2):222-224.
[13] 黄正刚,陈启文,吴星恒,等.67例儿童急性白血病流式细胞术免疫分型的研究[J].实用临床医学,2008,9(5):87-90.
[14] Christine P,Hans MD,William G,et al. Usefulness of anti-CD117 in the flow cytometric analysis of acute leukemia[J]. Hematopathology,2002,117(2):301-305.
[15] Uçkan D,Hiçsönmez G,et al. CD34/CD117 co-expression in childhood acute leukemia. Leuk Res,2000,24(3):201-206.
[16] 饶若,金丽华,王述文,等. CD117在急性髓系白血病病程中的表达分析[J]. 现代预防医学,2012,39(6):1502-1505.

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备注/Memo

备注/Memo:
收稿日期:2014-01-23 作者简介:王艳,在读硕士研究生。 通信作者:张晓春,E-mail:451337437@ qq. Com
更新日期/Last Update: 2014-06-20