|本期目录/Table of Contents|

[1]薛皓予,刘文庆,尚明华,等.胶质瘤患者肿瘤组织中IGF2BP2的表达及临床意义[J].宁夏医科大学学报,2014,(05):487-490,598.
 XUE Haoyu,LIU Wenqing,SHANG Minghua,et al.The Expression and Clinical Significance of IGF2BP2 Gene in Tumor Tissues of Patients with Glioma[J].Ningxia Medical University,2014,(05):487-490,598.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2014年05期
页码:
487-490,598
栏目:
论著
出版日期:
2014-05-30

文章信息/Info

Title:
The Expression and Clinical Significance of IGF2BP2 Gene in Tumor Tissues of Patients with Glioma
作者:
薛皓予1刘文庆2尚明华1王文斐1刘仲涛2田继辉2
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院神经外科,银川 750004
Author(s):
XUE Haoyu1LIU Wenqing2SHANG Minghua1WANG Wenfei1LIU Zhongtao2TIAN Jihui1
1.Ningxia Med. Univ.,Yinchuan 750004; 2.Dept. of Neurosurgery,the General Hospital of Ningxia Med. Univ.,Yinchuan 750004
关键词:
胶质瘤免疫组化IGF2BP2实时荧光定量PCR
Keywords:
glioma immunohistochemical IGF2BP2 real-time PCR
分类号:
R739.41
DOI:
-
文献标志码:
A
摘要:
目的探讨胰岛素样生长因子2结合蛋白2(insulin-likegrowthfactor2bindingprotein2IGF2BP2)在脑胶质瘤中的表达状况。方法应用实时荧光定量PCR技术检测47例不同病理级别(9例Ⅱ级,21例Ⅲ级,17例Ⅳ级)胶质瘤组织IGF2BP2表达情况,同时采用免疫组化法检测47例肿瘤组织中IGF2BP2的表达情况。结果Ⅱ级胶质瘤IGF2BP2蛋白阳性表达率阳性率(100%)明显高于胶质瘤Ⅲ级(52.38%)(P=0.013)和Ⅳ级(11.76%)(P=0.000)胶质瘤Ⅲ级高于Ⅳ级(P=0.015);Ⅱ级胶质瘤IGF2BP2的基因表达量(0.075±0.020)高于Ⅲ级(0.047±0.011)(P=0.016)和Ⅳ级胶质瘤(0.026±0.004)(P=0.000),Ⅲ级胶质瘤IGF2BP2的基因表达量高于Ⅳ级胶质瘤(P=0.000)。结论在Ⅱ级胶质瘤组织中IGF2BP2的表达水平高,并且随着病理级别的增高表达减弱,推测IGF2BP2可能在胶质瘤的发生发展中扮演重要的角色。
Abstract:
Objective To explore the expression of insulin-like growth factor2 binding protein2(IGF2BP2)in tumor tissues of patients with glioma. Methods The expression of IGF2BP2 gene of different pathological grade glioma tissue(9 cases of Grade II,21 cases of Grade III,and 17 cases of Grade IV)were detected using real-time PCR technology while the expression of IGF2BP2 gene was detected using immunohistochemistry. Results Positive rate of IGF2BP2 protein expression in different levels of gliomas as followed: positive rate of levelⅡ was 100%,positive rate of level III was 52.38% and positive rate of level Ⅳ was only 11.76%. Positive rate in IGF2BP2 protein expression of grade II was significantly higher than that of grade III and grade IV(P<0.05)while positive rate in IGF2BP2 protein expression of grade III was significantly higher than that of grade IV(P<0.05). IGF2BP2 gene expression volume in different levels of gliomas as followed:the expression volume of levelⅡ was(0.075±0.020),the expression volume of level III was(0.047±0.011)and the expression volume of level Ⅳ was(0.026±0.004). Conclusion The IGF2BP2 expression may play an important role in glioma development process and it can be used for further study.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-08-25 基金项目:宁夏自然科学基金(NZ1209) 作者简介:薛皓予,男,医师,在读硕士研究生,从事神经外科专业。 E-mail:xuehaoyu1226@126.com 通信作者:田继辉,教授,硕士研究生导师,研究方向:颅脑肿瘤。 E-mail:nxtjh1968@163.com
更新日期/Last Update: 2014-05-20