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[1]申淑珍,白晓川,马云,等.丹参酮ⅡA联合三氧化二砷对NB4细胞凋亡的影响[J].宁夏医科大学学报,2014,(02):178-182,190.
 SHEN Shuzhen,BAI Xiaochuan,MA Yun.Induction of Apoptosis in NB4 Cells by Tanshinone ⅡA Combination with Arsenic Trioxide[J].Ningxia Medical University,2014,(02):178-182,190.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2014年02期
页码:
178-182,190
栏目:
论著
出版日期:
2014-02-28

文章信息/Info

Title:
Induction of Apoptosis in NB4 Cells by Tanshinone ⅡA Combination with Arsenic Trioxide
作者:
申淑珍白晓川马云
宁夏医科大学总医院血液科,银川 750004
Author(s):
SHEN ShuzhenBAI XiaochuanMA Yun
Dept. of Hematology, the General Hospital of Ningxia Med.Univ.,Yinchuan 750004
关键词:
丹参酮ⅡA三氧化二砷细胞凋亡NB4细胞AnnexinV-FITC/PIp53Bcl-2
Keywords:
Tanshinone IIA arsenic trioxide Apoptosis NB4 cell line Annexin V-FITC/PI P53 Bcl-2
分类号:
R733.7
DOI:
-
文献标志码:
A
摘要:
目的观察丹参酮ⅡA(Tanshinone,TanⅡA)联合三氧化二砷(ATO)对人急性早幼粒细胞白血病细胞株(NB4细胞)凋亡的作用;并通过观察两药联合诱导NB4细胞凋亡时p53、Bcl-2表达的变化,探讨其可能的机制。方法通过AnnexinVFITC/PI荧光染色观察1.0μg·mL-1TanⅡA分别联合0.25、0.5、1.0μmol·L-1的ATO作用于NB4细胞的形态学变化;流式细胞术AnnexinV-FITC/PI法测定各组细胞凋亡率;流式细胞仪检测各组细胞p53、Bcl-2的表达。结果(1)1.0μg·mL-1TanⅡA分别与0.5、1.0μmol·L-1的ATO联合作用NB4细胞24、48和72h的凋亡率均较相应单独用药组凋亡率明显增高(P<0.05);染色荧光显微镜观察l.0μg·mL-1TanⅡA联合1.0μmol·L-1ATO实验组较1.0μmol·L-1ATO对照组72h时更易见到中晚期凋亡细胞和死亡细胞;(2)1.0μg·mL-1TanⅡA与0.5、1.0μmol·L-1的ATO联合作用NB4细胞48h、72h表达p53蛋白的细胞较1.0μmol·L-1ATO单药组明显增多(P<0.05),作用高峰时间为72h;1.0μg·mL-1TanⅡA分别与0.25、0.5、1.0μmol·L-1的ATO联合作用NB4细胞24h时,表达Bcl-2蛋白的细胞数较1.0μmol·L-1ATO单药组显著下降(P<0.05),且与ATO的浓度呈负相关(r=-0.858,P=0.000)。结论联合1.0μg·mL-1TanⅡA可增强0.5、1.0μmol·L-1ATO诱导NB4细胞凋亡;TanⅡA联合ATO诱导NB4细胞p53表达增强和Bcl-2表达减少可能是其促凋亡机制之一。
Abstract:
Objective To investigate the apoptosis effect induced by Tanshinone IIA(Tan IIA)combined with arsenic trioxide(ATO)in human acute promyelocytic leukemia(APL)cell line NB4 cells and to explore the potential mechanism by detecting the expression changes of P53 and Bcl-2. Methods The NB4 cells were treated with 1.0μg·mL-1 Tan IIA combined with ATO 0.25μmol·L-1,0.5μmol·L-1 and 1.0μmol·L-1 respectively. The morphological changes of the cells were demonstrated by Annexin V -FITC/PI immunocytochemistry and the expression of P53 and Bcl-2 was measured by flow cytometry(FCM). Results(1)The proliferative rate of NB4 cells treated by 1.0μg·mL-1 Tan II A combined with 1.0μmol·L-1 and 0.5μmol·L-1 ATO respectively during 24,48 and 72h was significantly inhibited compared with those treated by corresponding ATO alone group(P<0.05). 1.0μg·mL-1 Tan II A combined with 1.0μmol·L-1ATO was more easily to see middle to late period apoptosis cells and dead cells compared with 1.0μmol·L-1ATO alone group at 72h by Annexin V-FITC/PI immunocytochemistry study under fluorescence microscopy.(2)The expression of P53 in these two groups 1.0μg·mL-1 Tan II A combined with 0.5μmol·L-1 and 1.0μmol·L-1 ATO was significantly higher than that in ATO alone group at 48h and 72h(P<0.05)and the effect reached peak at 72h. The expression of Bcl-2 was significantly decreased in 1.0μg·mL-1 Tan II A combined with all different dose of ATO at 24h compared with 1.0μmol·L-1ATO alone group(P<0.05). ATO concentration was significantly negatively correlated to Bcl-2(r=-0.858, P=0.000). Conclusion Combination treatment of 1.0μg·mL-1 Tan II with 0.5,1.0μmol·L-1 ATO has significant synergistic effects on the apoptosis of NB4 cells. The synergistic effects of Tan IIA and ATO induced NB4 cells increase the expression of p53 and decrease the expression of Bcl-2, which maybe one of the mechanism of APL cell apoptosis.

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备注/Memo

备注/Memo:
收稿日期:2013-05-07 基金项目:宁夏医科大学重点孵育项目(XZ201010) 作者简介:申淑珍,女,硕士,主任医师,从事临床血液工作及恶性血液病研究。 通信作者:白晓川,男,教授,主任医师,从事临床血液工作及恶性血液病研究。E-mail:xiaochuan-5501@163.com
更新日期/Last Update: 2014-02-20