|本期目录/Table of Contents|

[1]李健挺,拓红安,王云飞,等.2型糖尿病大鼠骨折愈合期血清BMP-2、TNF-a和IL-1含量的变化[J].宁夏医科大学学报,2013,(12):1348-1351,1300.
 LI Jianting,TUO Hongan,WANG Yunfei,et al.Concentration Changes of Serum BMP-2, TNF-a, IL-1 during Fracture Healing in Rats with Type 2 Diabetes Mellitus in Vivo[J].Ningxia Medical University,2013,(12):1348-1351,1300.
点击复制

2型糖尿病大鼠骨折愈合期血清BMP-2、TNF-a和IL-1含量的变化(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年12期
页码:
1348-1351,1300
栏目:
论著
出版日期:
2013-12-20

文章信息/Info

Title:
Concentration Changes of Serum BMP-2, TNF-a, IL-1 during Fracture Healing in Rats with Type 2 Diabetes Mellitus in Vivo
作者:
李健挺;拓红安;王云飞;苗振普;马建民;王海龙;
宁夏银川市第二人民医院,银川 750011
Author(s):
LI Jianting TUO Hongan WANG Yunfei MIAO Zhengpu MA Jianmin WANG Hailong
LI Jianting, TUO Hongan, WANG Yunfei, MIAO Zhengpu, MA Jianmin, WANG Hailong
关键词:
2型糖尿病骨折BMP-2TNF-aIL-1
Keywords:
type 2 diabetes fracture BMP-2 TNF-a IL-1
分类号:
Q956
DOI:
-
文献标志码:
A
摘要:
目的探讨2型糖尿病大鼠骨折愈合过程与体内血清中BMP-2、TNF-a、IL-1变化关系的研究。方法取清洁近交SD雄性大鼠随机分为两组;正常对照组(NC)和糖尿病组(DM),DM组以高脂高糖饲料喂养10周联合腹腔注射小剂量链脲佐菌素的方法建立2型糖尿病大鼠模型后,折断两组大鼠右胫骨并复位外固定。分别于骨折后第1、3、5、8周拍X线片观察骨折愈合情况;同时尾静脉取血并留取血清,用ELISA方法检测2组血清中BMP-2、TNF-a、IL-1水平。结果骨折后第1、3、5、8周X线片观察NC组骨折愈合好于DM组;DM组骨折后血清BMP-2浓度显著低于NC组,而血清TNF-a与IL-1浓度显著高于NC组。结论糖尿病大鼠骨折后血清BMP-2减少、TNF-a和IL-1增加与其骨折愈合差密切相关。
Abstract:
Objective To observe the concentration changes of serum BMP-2, TNF-a, IL-1 during fracture healing in rats with type 2 diabetes mellitus in vivo. Methods The clean inbred Sprague-daw Ley(SD)male rats were randomly divided into two groups: the normal control group(NC)and diabetic group(DM). The DM group rats were fed with high fat and sucrose diet for 10 weeks,then injected with small dose streptozotocin by intraperitoneal to establish the rat model of type 2 diabetes. The right tibia of two group's rats were broken, reset and externally fixed. The fracture healing was observed with X-ray, while serum levels of BMP-2, TNF-a and IL-1 were detected by ELISA. Results X-ray films showed that the fracture healing of normal control group was significantly better than that of diabetic group. In DM group, the levels of BMP-2 decreased significantly(P<0.05 or P<0.01)as compared with NC group, while levels of TNF-a and IL-1 increased significantly(P<0.05 or P<0.01)as compared with NC group. Conclusion The unfavorable fracture healing of rats with type 2 diabetes mellitus was associated with the BMP-2 reduction and high serum levels of TNF-a and IL-1.

参考文献/References:

[1] De Carvalho MH, Colao AL, Fortes ZB. Cytokines, endothelialdys function, and insulin resistance[J]. Arq Bras Endocrinol Metabol, 2006, 50(2):304-312.
[2] Cowan CM,Aalami OO,Shi YY,et al.Bone morphogenetic protein 2 and retinoic acid accelerate in vivo bone formation, osteoclast recruitment,and bone turnover[J]. Tissue Eng, 2005, 11(3-4): 645-658.
[3] Zuscik MJ,Ma L,Buckley T, et al.Lead induces chondrogenesis and alters transforming growth factor-beta and bone morphogenetic protein signaling in mesenchymal cell populations[J]. Environ Health Perspect, 2007,115(9):1276-1282.
[4] 孙婵,王凌,李大金.细胞因子对破骨细胞生物学功能的调控作用[J].现代免疫学,2008,28(3):261-264.
[5] 叶夏云,钟建庭.交叉反应蛋白、白细胞介素6、肿瘤坏死因子α 与糖尿病肾病的关系研究[J].中国现在医学杂志,2007,17(6):704-708.
[6] Yang W.Prevalence of diabetes among men and women in China[J].N Engl J Med,2010,362(12):1090-1101.
[7] 佘国荣,李劫若.合并糖尿病的骨折愈合影响因素研究进展[J].中国矫形外科杂志,2011,22(3):143-144.
[8] 祁燕,曾小云,李凯利,等.炎症在2型糖尿病大鼠胰岛素抵抗中的作用[J].中国现代医学杂志,2009,19(16):2453-2455.
[9] 张纲,李焰,谭颖徽.细胞生长因子在骨折愈合中的作用研究进展[J].重庆医学,2008,37(2):196-197.
[10] 孙贤杰.骨折愈合的影响因素[J].中国医药指南,2012,17(10):82-84.
[11] Srivastava S, Mchugh K, Kimble R, et al. Estrogen downregulates the expression of M-CSF mRNA in bone marrow stromal cells by stimulating the production of the transcription factor EGF-1[J]. Bone Miner Res, 1995, 10(Suppl 1):18.
[12] Xiao E, Xia-Zhang L, Ferin M, et al. Inhibitory effects of endotoxin on LH secretion in the ovariectomized monkey are prevented by naloxone but not by an interleukin-1 receptor antagonist[J]. Neuroimmunomodulation,2000,7(1):6-15.

相似文献/References:

[1]张炜,吴义刚,庞建忠,等.应用MIPPO技术结合锁定钢板治疗胫骨远段骨折[J].宁夏医科大学学报,2013,(04):457.
[2]李军.动力髋螺钉和人工股骨头置换对老年股骨粗隆间骨折患者的疗效分析[J].宁夏医科大学学报,2013,(03):331.
[3]林滢宇.不同手术入路治疗肱骨髁间骨折临床效果比较[J].宁夏医科大学学报,2013,(03):329.
[4]汪玉海,金丽娟,高 俊,等.脂肪干细胞复合PLGA对骨质疏松骨折愈合后生物力学的影响[J].宁夏医科大学学报,2013,(03):244.
 WANG Yu-hai,JIN Li-juan,GAO Jun,et al.Effect of Adipose Derived Stem Cells in PLGA on Biomechanics after Osteoporotic Fracture-healing[J].Ningxia Medical University,2013,(12):244.
[5]邱渝江,王忠平,姜光财,等.28例空心加压螺钉内固定治疗股骨颈骨折早期失败原因及对策[J].宁夏医科大学学报,2013,(02):220.
[6]杜雪雪,李 瑞,张惠英.2型糖尿病患者血清氧化应激水平及相关微量元素分析[J].宁夏医科大学学报,2013,(01):59.
 DU Xue-xue,LI Rui,ZHANG Hui-ying.Oxidative Stress Status and its Related Trace Elements in Patients with Type 2 Diabetes[J].Ningxia Medical University,2013,(12):59.
[7]王 平,王彦平,王一农.解剖锁定钢板治疗老年股骨转子间粉碎性骨折28例报道[J].宁夏医科大学学报,2013,(09):1038.
[8]童 锋,徐 灵,张 磊,等.老年股骨转子间骨折锁定钢板治疗体会[J].宁夏医科大学学报,2013,(09):1035.
[9]戴涟生,荆 浩,程 飞.切开复位内固定术与闭合复位内固定术治疗肱骨髁上骨折的临床对比研究[J].宁夏医科大学学报,2013,(09):1010.
[10]胡旭珍,吴晓晶,马治国,等.回药伊消方改善2型糖尿病胰岛素抵抗作用的临床研究[J].宁夏医科大学学报,2013,(09):953.
 YANG Xiaoming,LI Xiaobo,et al.Inhibit Effect of SULT2B1 Interference on Proliferation Ability of Mouse H22 Hepatocellular Carcinoma Cells[J].Ningxia Medical University,2013,(12):953.

备注/Memo

备注/Memo:
收稿日期:2013-08-27 基金项目:宁夏卫生厅重点项目(2011046) 作者简介:李健挺,男,副主任医师,从事临床骨伤专业。
更新日期/Last Update: 2013-12-20