|本期目录/Table of Contents|

[1]张淑雅,陈 昕,桑旭东,等.胶原盘状结构域受体2基因缺失对小鼠卵巢功能的影响[J].宁夏医科大学学报,2013,(12):1333-1335,1344,1303.
 ZHANG Shuya,CHEN Xin,SANG Xudong,et al.The Effect of Discoidin Domain Receptor 2 Deficiency on Ovarian Function in Mice[J].Ningxia Medical University,2013,(12):1333-1335,1344,1303.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年12期
页码:
1333-1335,1344,1303
栏目:
论著
出版日期:
2013-12-20

文章信息/Info

Title:
The Effect of Discoidin Domain Receptor 2 Deficiency on Ovarian Function in Mice
作者:
张淑雅1 陈 昕1 桑旭东2 卜 歆2 王燕蓉3 苏 金2 裴秀英1
1.宁夏医科大学生育力保持教育部重点实验室/生物化学与分子生物学系,银川 750004; 2.第四军医大学生物化学与分子生物学系,西安 730021; 3.宁夏医科大学生育力保持教育部重点实验室/ 组胚学教研室,银川 750004
Author(s):
ZHANG Shuya1 CHEN Xin1 SANG Xudong2 BU Xin2 WANG Yanrong1 SU Jin2 PEI Xiuying1
1.Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004; 2.Department of Biochemistry and Molecular Biology, the Fourth Military Medical University, Xi'an 710032
关键词:
细胞外基质胶原盘状结构域受体2黄体不孕
Keywords:
extracellular matrix discoindin domain receptor corpus luteum infertility
分类号:
Q95-3
DOI:
-
文献标志码:
A
摘要:
目的利用胶原盘状结构域受体2(Discoidindomainreceptor2,DDR2)基因缺失小鼠,研究DDR2缺失对卵巢功能的影响,探索DDR2缺失小鼠不孕的原因。方法①阴道脱落细胞涂片结合改良巴氏染色法检测DDR2基因缺失纯合子(SLIE)与野生型(WT)小鼠动情周期的差异。②PMSG+HCG促排卵后在体式显微镜下计数排卵数量差异,HE染色法观察SLIE小鼠与WT小鼠促排卵后卵巢与子宫形态及结构的差异。结果①成年SLIE小鼠缺乏明显的动情期,绝大多数处于动情间期,缺乏规律的动情周期。②促排卵实验发现,SLIE小鼠促排卵数量显著减少;③排卵后黄体生成早期,SLIE小鼠子宫壁薄,体积小;卵巢切片中没有黄体生成;子宫切片HE染色显示子宫内膜薄,腺体及间质增生不明显,未见分泌粘液,未能进入分泌期。结论DDR2缺失导致卵巢对促性腺激素的反应能力降低,促排卵后黄体生成障碍。
Abstract:
Objective To make a new understanding of extracellular matrix function on ovary and to provide new clues for the diagnosis and treatment of infertility. Methods ① Estrus cycle of mice were detected using vaginal exfoliated cells smear method. ② The structure and morphology of ovary and uterus were observed by HE staining. Results ① During sexual maturity period, the slie mice had a disorder oestrous cycle, lacking of obvious oestrus and the vast majority in estrogen interphase. ② The administration of exogenous gonadotropins induced follicular growth to the Graffian stage. It failed to alter the anovulatory phenotype of slie homozygotes, full luteinization of the thecal cells did not occur and no corpora lutea were formed. All these induced Endometrial development disorder. Conclusion Ddr2 deficiency lead to the ability of mice ovarian response to gonadotropin declined, ovulation and corpus luteum formation obstacle even after the administration of exogenous gonadotropins.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-09-11 基金项目:国家自然科学基金(81360094,81260110); 宁夏教育厅重点项目(宁教高[2011]263) 作者简介:张淑雅(1981-),女,博士,讲师,从事生理与病理性血管新生研究。E-mail:zhangshuya1268@163.com 通信作者:裴秀英。E-mail:Peixiuying@163.com
更新日期/Last Update: 2013-12-20