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[1]杨晓明,李晓波,殷莲华.干扰SULT2B1对Hepa1-6肝癌细胞凋亡的影响及机制研究[J].宁夏医科大学学报,2013,(11):1208-1211,1309.
 Effect of SULTB Interference on Apoptosis of Mouse Hepa- Hepatocellular Carcinoma Cells and Its Underlying Mechanism.Effect of SULT2B1 Interference on Apoptosis of Mouse Hepa1-6 Hepatocellular Carcinoma Cells and Its Underlying Mechanism[J].Ningxia Medical University,2013,(11):1208-1211,1309.
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干扰SULT2B1对Hepa1-6肝癌细胞凋亡的影响及机制研究(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年11期
页码:
1208-1211,1309
栏目:
论著
出版日期:
2013-11-20

文章信息/Info

Title:
Effect of SULT2B1 Interference on Apoptosis of Mouse Hepa1-6 Hepatocellular Carcinoma Cells and Its Underlying Mechanism
作者:
杨晓明12 李晓波2 殷莲华2
1.宁夏医科大学,银川 750004; 2.复旦大学上海医学院,上海 200032
Author(s):
Effect of SULT2B1 Interference on Apoptosis of Mouse Hepa1-6 Hepatocellular Carcinoma Cells and Its Underlying Mechanism
1.Ningxia Medical University, Yinchuan 750004; 2.School of Shanghai Medical College, Fudan University, Shanghai 200032
关键词:
肝癌细胞羟基类固醇硫酸基转移酶SULT2B1细胞凋亡
Keywords:
hepatocellular carcinoma cell hydroxysteroid sulfotransferases 2B1 cell apoptosis
分类号:
R735.7
DOI:
-
文献标志码:
A
摘要:
目的研究羟基类固醇硫酸基转移酶SULT2B1对小鼠Hepa1-6肝癌细胞凋亡的影响及作用机制。方法SULT2B1慢病毒干扰载体感染Hepa1-6细胞(MOI=100),Real-timePCR检测其干扰效果。采用流式细胞仪通过AnnexinV-APC/7-ADD染色检测干扰SULT2B1后Hepa1-6细胞凋亡率的改变,Westernblot检测FAS和p-JNK的蛋白表达水平。结果构建的SULT2B1干扰载体可显著抑制Hepa1-6细胞中SULT2B1的表达(P<0.05)。Hepa1-6细胞中干扰SULT2B1后其凋亡率较对照组相比显著增加(P<0.05),干扰SULT2B1后可显著增加FAS和p-JNK的蛋白表达水平(P<0.05)。结论干扰SULT2B1可促进Hepa1-6细胞的凋亡,其作用主要与增加FAS和p-JNK的蛋白表达水平有关。
Abstract:
Objective To investigate the effect and possible mechanism of hydroxysteroid sulfotransferase 2B1(SULT2B1)on cell apoptosis of mouse hepatocellular carcinoma Hepa1-6 cells. Methods Hepa1-6 cells were infected with lentivirus-mediated SULT2B1 interference vectors at multiplicity of infection(MOI)of 100. The mRNA levels of SULT2B1 in Hepa1-6 cells treated with vectors were detected by real-time PCR analysis. The ratio of apoptotic cells were analyzed by flow cytometry after incubated with Annexin V-APC and 7-ADD. FAS and p-JNK protein levels were analyzed by western blot assay. Results SULT2B1 expression was inhibited significantly in Hepa1-6 cells with constructed SULT2B1 interference vector(P<0.05). The percentage of apoptosis was significantly increased in Hepa1-6 with SULT2B1 interference, compared with control cells(P<0.05). Moreover, the protein levels of FAS and p-JNK were increased significantly in Hepa1-6 cells with SULT2B1 knock-down(P<0.05). Conclusion Knock-down of SULT2B1 induced Hepa1-6 cell apoptosis, which mechanism was mainly associated with up-regulation of FAS and p-JNK protein levels.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-06-27 基金项目:国家自然科学基金(30800547); 宁夏卫生厅项目(2009031); 宁夏医科大学面上项目(XM200920 ) 作者简介:杨晓明,女,讲师,博士。E-mail:xmyang@hotmail.com
更新日期/Last Update: 2013-11-20