|本期目录/Table of Contents|

[1]王春生,孟尽海,吴 燕,等.丙泊酚对大鼠急性脊髓损伤早期的影响[J].宁夏医科大学学报,2013,(09):964-967.
 WANG Chunsheng,MENG Jinhai,WU Yan,et al.Effects of Propofol on Acute Early Spinal Cord Injury in Rats[J].Ningxia Medical University,2013,(09):964-967.
点击复制

丙泊酚对大鼠急性脊髓损伤早期的影响(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年09期
页码:
964-967
栏目:
论著
出版日期:
2013-09-30

文章信息/Info

Title:
Effects of Propofol on Acute Early Spinal Cord Injury in Rats
作者:
王春生1 孟尽海2 吴 燕1 刘 斐1 马 万1
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院麻醉科,银川 750004
Author(s):
WANG Chunsheng1 MENG Jinhai2 WU Yan1 LIU Fei1 MA Wan1
1.Ningxia Med. Univ., Yinchuan 750004; 2.Dept. of Anesthesiology,the General Hospital of Ningxia Med. Univ., Yinchuan 750004
关键词:
丙泊酚 脊髓损伤 血清S100β蛋白 脊髓组织总钙
Keywords:
propofol spinal cord injury early effects
分类号:
R614
DOI:
-
文献标志码:
A
摘要:
目的 观察丙泊酚对大鼠急性脊髓损伤早期的影响,探讨丙泊酚对急性脊髓损伤早期脊髓神经组织的保护作用。方法 健康雄性SD大鼠48只随机分为6组,每组8只。假手术组(S组)、脊髓损伤组(I组)、脂肪乳组(F组)和不同剂量丙泊酚组:低剂量组(L组)、中剂量组(M组)和高剂量组(H组)。S组仅切除T9全椎板,不损伤脊髓; 其余各组应用Allen,s重物坠落法并加以改良制备大鼠急性脊髓损伤模型。各组药物干预15min(T15)和60min(T60)时取动脉血大鼠1.5mL测血清S100β蛋白含量,各组药物干预60min后立即断头处死大鼠取脊髓组织测脊髓含水量及脊髓组织总钙含量。结果 与S组比较,其余五组在T15和T60时血清S100β蛋白和脊髓组织总钙含量均明显升高(P均<0.01),I组、F组、L组和H组脊髓含水量均增多(P均<0.05); I组与F组间各时间点血清S100β蛋白含量、脊髓组织含水量及总钙含量差异均无统计学意义(P>0.05); 与I组和F组分别比较,在T15和T60时L组、M组和H组血清S100β蛋白和脊髓组织总钙含量均显著降低(P均<0.01),M组脊髓含水量低于I组和F组(P<0.05); 与L组比较,在T15和T60时M组和H组血清S100β蛋白和脊髓组织总钙含量均降低(P均<0.01); 与M组比较,在T15和T60时H组血清S100β蛋白和脊髓组织总钙含量均升高(P均<0.01),脊髓含水量增多(P<0.05); 与T15时比较,L组、M组和H组在T60时血清S100β蛋白含量均明显降低(P均<0.01)。结论 丙泊酚34.92mg·(kg·h)-1持续泵注60min对急性脊髓损伤后早期有保护作用。
Abstract:
Objective To investigate he effects of propofol on acute early spinal cord injury, and to explore the protective effect of propofol on the early acute spinal cord injury spinal cord nerve tissue. Methods 48 healthy male SD rats were randomly divided into six groups with 8 rats each(n=8),sham group(group S), spinal cord injury group(group I), fat emulsion group(group F)and different concentrations of propofol groups(group L,M,H). T9 laminectomy was resected with no damage to the spinal cord in group S.Acute spinal cord injury model was established with Allen's fall method in rats of rest groups.At the drug intervention 15min(T15)and 60min(T60),1.5mL blood samples were collected for determination S100β protein contents.The rats were sacrificed immediately after the drug intervention 60min. Spinal cord water contents and total calcium of spinal cord tissues were measured. Results Compared with group S, S100βprotein contents and total calcium of spinal cord tissue in the other five groups significantly increased at T15 and T60(P<0.01)while the spinal cord water content increased in groups I, S, L and H(P<0.05); Compared with group I, S100βProtein content, total calcium of spinal cord tissue and the spinal cord water content no significant change were found in group F(P>0.05); Compared with group I and F, S100βProtein content and total calcium of spinal cord tissue in groups L, M and H significantly decreased at T15 and T60(P<0.01)while the spinal cord water content decreased in group M(P<0.05); Compared with group L, S100βProtein content and total calcium of spinal cord tissue in groups M and H decreased at T15 and T60(P<0.01); Compared with group M, S100βProtein content and total calcium of spinal cord tissue in group H increased at T15 and T60(P<0.01)while the spinal cord water content increased in group H(P<0.05); Compared with T15,S100βProtein content in groups L, M and H decreased at T60(P<0.01). Conclusion Propofol[34.92mg·(kg·h)-1]continuous infusion 60min has a protective effect on early acute spinal cord injury in rats.

参考文献/References:

[1] Albert T,Ravaud JF,Papa A,et al.Rehabilitation of spinal cord injury in France:a nationwide multicentre study of incidence and regional disparities[J].Spinal cord, 2005,43(6):357-365.
[2] Sakai H,Sheng H,Yates RB,et al.Isoflurane provides long-term protection against focal cerebral ischemia in the rat[J].Anesthesiology, 2007,106(1):92-99.
[3] Yu QJ,Hu J,Yang J,et al.Protective effect of propofol preconditioning and postconditioning against ischemic spinal cord injury[J]. Neural Regeneration Research,2011,6(12):951-955.
[4] Allen AR.Surgery of experimental lesions of spinal cord equivalent to crush injury of fracture dislocation preliminary report[J].JAMA, 1911,57:878-880.
[5] 宋娟娥,于水.电感耦合等离子体质谱法在临床样品分析中的应用[J].质谱学报,2006,27(3):182-192.
[6] Rabinowitz RS,Eck JC,Harper CM,et al.Urgent surgical decompression compared to methylprednisolone for the treatment of acute spinal cord injury[J].Spine,2008, 33(21):2260-2268.
[7] Miyanji F,Furlan JC,Aarabi B,et al.Acute cervical traumatic spinal cord injury:MR imaging findings correlated with neurologic outcome- prospective study with 100 consecutive patients[J].Radiology,2007,243(3):820-827.
[8] Buttner T,Weyers S,Postert T,et al.S-100 protein:serum marker of focal brain damage after ischemic territorial MCA infarction[J].Stroke, 1997,28(10):1961-1965.
[9] 邹以席,黄方炯,吴海燕,等.胸腹主动脉瘤手术中枢神经系统损伤标记物临床研究[J].中华实用诊断与治疗杂志,2010,24(4):357.
[10] Woertgen C,Rotherl RD,Metz C,et al.Comparison of clinical, radiologic,and serum maker as prognostic factors after severe head injury[J].Trauma,2000,49(3):570-571.
[11] Bano D,Nicotera P.Ca2+ signals and neuronal death in brain ischemia[J].Stroke, 2007,38(2):674-676.

相似文献/References:

[1]任雪艳,孟尽海.丙泊酚对创伤家兔凝血功能的影响[J].宁夏医科大学学报,2009,(02):165.
 REN Xue-yan,MENG Jin-hai.[J].Ningxia Medical University,2009,(09):165.
[2]王锋,张雪芹.丙泊酚复合芬太尼、舒芬太尼、瑞芬太尼用于无痛人流的对比观察[J].宁夏医科大学学报,2010,(03):401.
[3]徐淑芳,杨学锋,蒋晓敏,等.丙泊酚复合舒芬太尼用于无痛人工流产术的效果观察[J].宁夏医科大学学报,2012,(11):1181.
[4]马刚,孟尽海,陈进华,等.丙泊酚在体外循环中的脑保护作用研究进展[J].宁夏医科大学学报,2012,(07):750.
[5]闫瑞,马增瑞,陈学新.血氨对肝硬化大鼠丙泊酚麻醉苏醒时间的影响[J].宁夏医科大学学报,2012,(05):459.
 YAN Rui,MA Zeng-rui,CHEN Xue-xin.Effect of Blood Ammonia on Awaking Time of Propofol Anesthesia in Rats with Hepatic Cirrhosis[J].Ningxia Medical University,2012,(09):459.
[6]雷庆红,雷小红,陈进华,等.不同剂量丙泊酚对体外循环期间血管紧张素Ⅱ的影响[J].宁夏医科大学学报,2012,(04):377.
[7]陈学新,闫瑞,李振洲,等.吸入30%~40%浓度氧对肝硬化大鼠静注丙泊酚镇静效应的影响[J].宁夏医科大学学报,2013,(11):1223.
 CHEN Xuexin,YAN Rui,LI Zhenzhou,et al.The Sedative Effect of Inhaling 30%~40% Oxygen on Cirrhosis Rats Induced by Intravenous Injection of Propofol[J].Ningxia Medical University,2013,(09):1223.
[8]刘 瑾,徐仙彬,马 峰.丙泊酚复合地佐辛用于无痛人工流产术的临床观察[J].宁夏医科大学学报,2013,(10):1176.
[9]赵 玲,盛旭东,明 婷,等.丙泊酚对大鼠局灶性脑缺血再灌注后AQP-4mRNA表达的影响[J].宁夏医科大学学报,2013,(10):1083.
 ZHAO Ling,SHENG Xudong,MING Ting,et al.Effects of Propofol on Expression of AQP-4 in Rat Models of Transient Focal Ischemia-reperfusion[J].Ningxia Medical University,2013,(09):1083.
[10]李 静,王文娟,曾玲双.丙泊酚对心肌肥厚大鼠心肌肌浆网Ca2+-ATPase活性的影响[J].宁夏医科大学学报,2013,(02):146.
 LI Jing,WANG Wen-juan,ZENG Ling-shuang.Effects of Propofol on Ca2+-ATPase Activity of Myocardial Plasma Membrane in Cardiac Hypertrophy Rats[J].Ningxia Medical University,2013,(09):146.

备注/Memo

备注/Memo:
收稿日期:2013-04-27 基金项目:宁夏自然科学基金(NZ11183) 作者简介:王春生(1985-),男,宁夏人,在读硕士研究生,麻醉学专业。
更新日期/Last Update: 2013-09-20