|本期目录/Table of Contents|

[1]李云鸿,齐 奇,滕 鹏,等.热休克蛋白60在激活的少突胶质前体细胞的表达[J].宁夏医科大学学报,2013,(09):960-963.
 LI Yunhong,QI Qi,TENG Peng,et al.Expression of HSP60 in Activated OPC[J].Ningxia Medical University,2013,(09):960-963.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年09期
页码:
960-963
栏目:
论著
出版日期:
2021-09-15

文章信息/Info

Title:
Expression of HSP60 in Activated OPC
作者:
李云鸿1 齐 奇1 滕 鹏1 成文静1 马 骄1 张 楠1 丁斐嘉1
1.宁夏医科大学,银川 750004; 2.浙江大学医学部神经生物学系,杭州 310058
Author(s):
LI Yunhong QI Qi TENG Peng CHENG Wenjing MA Jiao ZHANG Nan DING Feijia LI Fan MIAO Zhenhua HUANG Weidong SHEN Ying WANG Yin
Ningxia Medical University,Yinchuan 750004
关键词:
热休克蛋白60 少突胶质细胞前体细胞 重组人干扰素-γ 重组人热休克蛋白60
Keywords:
HSP60 OPC IFN-γ rhHSP60
分类号:
R322.81
DOI:
-
文献标志码:
A
摘要:
目的 研究热休克蛋白60(HSP60)在重组人干扰素-γ(IFN-γ)和重组人热休克蛋白60(rhHSP60)激活的少突胶质前体细胞中的表达情况。方法 分离SD大鼠的少突胶质细胞前体细胞(OPC),经IFN-γ和 rhHSP60激活后,应用western blot方法检测细胞中的HSP60蛋白的表达; 采用ELISA方法检测细胞培养液中炎症因子TNF-α及IL-6的释放量。结果 经IFN-γ和 rhHSP60分别激活后,OPC胞体内的HSP60蛋白表达均显著升高,胞外TNF-α及IL-6的释放量也明显增加。结论 在激活状态下,OPC中的HSP60蛋白表达显著增高,刺激细胞产生大量炎症因子,从而参与细胞的应激反应。
Abstract:
Objective To explore the expression of HSP60 in IFN-γ and rhHSP60 activated OPC.Methods After OPC were treated with IFN-γ and rhHSP60, cells were collected for western-blot.The release of TNF-α and IL-6 were detected by ELISA in the supernatant. Results In IFN-γ and rhHSP60 activated OPC,HSP60 protein significantly increased and stimulated the production of amount of cytokines such as TNF-α and IL-6 compared to control.Conclusion In activated OPC,HSP60 highly expressed to participate the stress response.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-02-13 基金项目:国家自然科学基金项目(31260243,31060140),教育部新世纪优秀人才支持计划和留学回国人员基金 作者简介:李云鸿,男,讲师,硕士,从事神经生物学研究。
更新日期/Last Update: 2013-09-20