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[1]胡旭珍,吴晓晶,马治国,等.回药伊消方改善2型糖尿病胰岛素抵抗作用的临床研究[J].宁夏医科大学学报,2013,(09):953-955.
 YANG Xiaoming,LI Xiaobo,et al.Inhibit Effect of SULT2B1 Interference on Proliferation Ability of Mouse H22 Hepatocellular Carcinoma Cells[J].Ningxia Medical University,2013,(09):953-955.
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回药伊消方改善2型糖尿病胰岛素抵抗作用的临床研究(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年09期
页码:
953-955
栏目:
回医药学
出版日期:
2013-09-30

文章信息/Info

Title:
Inhibit Effect of SULT2B1 Interference on Proliferation Ability of Mouse H22 Hepatocellular Carcinoma Cells
作者:
胡旭珍 吴晓晶 马治国 赵红波
宁夏医科大学附属银川市中医医院,银川 750001
Author(s):
YANG Xiaoming1 2 LI Xiaobo2 YIN Lianhua2
1.Ningxia Med. Univ., Yinchuan 750004; 2.Fudan Univ. Shanghai Med. Coll., Shanghai 200032
关键词:
回药伊消方 2型糖尿病 胰岛素抵抗
Keywords:
hepatocellular carcinoma hydroxysteroid sulfotransferases 2B1 cell proliferation
分类号:
R289.5
DOI:
-
文献标志码:
B
摘要:
目的 观察以益禀补肾、化痰通络为组方原则的回药伊消方对2型糖尿病患者胰岛素抵抗的疗效。方法 采用随机对照方法,将符合纳入标准的170例2型糖尿病患者分为观察组(85例)和对照组(85例)。两组患者均在糖尿病饮食的基础上,予二甲双胍口服,每次500mg,每日3次,于每次餐前 30min 温开水送服。观察组加用回药伊消方,两组均连续用药半年为一个疗程。观察治疗前后空腹血糖(FBG)、血清胰岛素(FIns),糖化血红蛋白(HbAlc),血脂(TC、TG、LDL、HDL),血液流变学指标(全血黏度、血浆比黏度、红细胞压积)的变化。结果 两组患者治疗后FBG、FIns、HbAlc均较治疗前明显降低(P<0.05或P<0.01),胰岛素敏感性指数(ISI)明显升高(P<0.05),且观察组疗效均优于对照组(P<0.05); 与治疗前比较,两组患者治疗后TG、TC、LDL-C明显降低(P<0.05或P<0.01),HDL-C明显升高(P<0.05),观察组TG明显低于对照组(P<0.05),TC、LDL-C、HDL-C两组间比较差异无统计学意义; 与同组治疗前比较,观察组治疗后患者的血流变学各指标均明显降低(P<0.05),与对照组比较各指标也显著降低(P<0.05)。结论 回药伊消方可明显降低2型糖尿病患者的血糖、改善血脂,增加胰岛素敏感性,纠正血液流变异常,改善2型糖尿病胰岛素抵抗状态。
Abstract:
Objective To investigate the inhibit effect of hydroxysteroid sulfotransferase 2B1(SULT2B1)on proliferation ability of mouse hepatocellular carcinoma H22 cells in vitro, and to explore the underlying mechanisms. Methods H22 cells were infected with lentivirus-mediated SULT2B1 interference vectors at multiplicity of infection(MOI)of 50 and 100. cell proliferation ability with SULT2B1 inhibition was assessed by CCK-8 assay. Besides, cell proliferation ability associated gene such as BCL2 and MYC were detected by real-time PCR and Western blot assays. Results H22 cell proliferation ability was inhibited following SULT2B1 interference at different time point(24 h, 48 h, 72 h)respectively(MOI=100)(P<0.05). SULT2B1 mRNA level decreased dramatically with SULT2B1-LV infection. In addition, The mRNA and protein levels of BCL2 and MYC were downregulated significantly in H22 cells infected wih SULT2B1-LV at MOI of 50 and 100(P<0.05). However, there were no significant differences between different MOI infection(P>0.05). Conclusion Knock-down of SULT2B1 can inhibit H22 cell proliferation ability in vitro, which role was associated with down-regulation of BCL2 and MYC levels.

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备注/Memo

备注/Memo:
收稿日期:2013-02-10 基金项目:宁夏自然科学基金项目(NZ11230) 作者简介:胡旭珍(1963-),女,主任医师,主要从糖尿病及其并发症的临床与研究及回族医药研究工作。
更新日期/Last Update: 2013-09-20