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[1]郭晖,王占伟,郝文炯,等.大鼠HSG基因重组腺病毒载体的构建及鉴定[J].宁夏医科大学学报,2013,(06):679-682.
 GUO Hui,WANG Zhan-wei,HAO Wen-jiong,et al.-[J].Ningxia Medical University,2013,(06):679-682.
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大鼠HSG基因重组腺病毒载体的构建及鉴定(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年06期
页码:
679-682
栏目:
论著
出版日期:
2013-06-20

文章信息/Info

Title:
-
作者:
郭晖;王占伟;郝文炯;赵巍;沈冰;
宁夏医科大学;宁夏医科大学总医院神经外科;郑州市第七人民医院神经外科;延安大学附属医院神经外科;宁夏医科大学医学科学技术研究中心;
Author(s):
GUO Hui12WANG Zhan-wei23HAO Wen-jiong4ZHAO Wei5SHEN Bing2
1.Ningxia Medical University,Yingchuan 750004;2.Department of Neurosurgery,the General Hospital of Ningxia Medical University,Yinchuan 750004;3.Zhengzhou No.7 People’s Hospital.Zhengzhou 450016;4.Department of Neurosurgery,the Affiliated Hospital of Yanan University,Yanan 716000; 5.Science and Technology Department of Ningxia Medical University,Yinchuan 750004
关键词:
增殖抑制基因腺病毒恶性胶质瘤
Keywords:
-
分类号:
R739.4
DOI:
-
文献标志码:
A
摘要:
目的构建大鼠增殖抑制基因(rathyperplasiasuppressorgene,rHSG)的腺病毒载体,观察其在转染大鼠C6胶质瘤细胞后的表达。方法由前期构建的pGM-T-rHSG质粒中酶切rHSG基因片段,亚克隆入pShut-tle-CMV-EGFP重组穿梭载体中,而后与腺病毒骨架质粒pAdxsi酶切连接,经过抗性筛选及酶切鉴定得到阳性的pAdxsi-GFP-rHSG重组质粒;pAdxsi-GFP-rHSG质粒经PacI线性化后转染293细胞,包装重组腺病毒,进行PCR鉴定和PCR产物测序鉴定,Western-blot法检测C6胶质瘤细胞转染rHSG后的表达效果。结果酶切鉴定证实rHSG基因正确插入穿梭载体pShuttle-CMV-EGFP,收获病毒后的PCR及测序鉴定pAdxsi-GFP-rHSG重组成功,并能在C6胶质瘤细胞内高效表达。结论成功构建出了介导大鼠增殖抑制基因的腺病毒载体pAdxsi-GFP-rHSG,能在C6胶质瘤细胞内高效表达。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
【基金】 宁夏教育厅资助项目;银川市科技攻关项目
更新日期/Last Update: 2013-06-20