|本期目录/Table of Contents|

[1]李张珂,李延辉,王 凡,等.大鼠胶质瘤细胞中生物钟基因Period2的生物节律性研究[J].宁夏医科大学学报,2013,(05):485-488,496.
 LI Zhang-ke,LI Yan-hui,WANG Fan,et al.The Biological Rhythms of CircadianClock Gene Period2 in Glioma Cells[J].Ningxia Medical University,2013,(05):485-488,496.
点击复制

大鼠胶质瘤细胞中生物钟基因Period2的生物节律性研究(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年05期
页码:
485-488,496
栏目:
论著
出版日期:
2013-05-20

文章信息/Info

Title:
The Biological Rhythms of CircadianClock Gene Period2 in Glioma Cells
作者:
李张珂14 李延辉1 王 凡2 尹 磊1 牛占峰3 夏鹤春34
1.宁夏医科大学,银川 750004; 2.荆门市第一人民医院,荆门 448000; 3.宁夏医科大学总医院,银川 750004; 4.宁夏颅脑疾病重点实验室,银川 750004
Author(s):
LI Zhang-ke1 LI Yan-hui14 WANG Fan2 YIN Lei1 NIU Zhan-feng3 XIA He-chun34
1.Ningxia Med. Univ.,Yinchuan 750004; 2.Dept. of Neurosurgery,the First Peoples' Hospital of Jingmen,Jingmen 488000; 3.Dept. of Neurosurgery, the General Hospital of Ningxia Med. Univ., Yinchuan 750004; 4.Incubation Base of National key Laboratory for Cerebrocranial Diseases, Yinchuan 750004
关键词:
Per2 胶质瘤 生物节律性
Keywords:
Per2 glioma biological rhythm
分类号:
R73-3
DOI:
-
文献标志码:
A
摘要:
目的 揭示生物钟基因Period2(Per2)在鼠胶质瘤C6细胞内周期表达规律。方法 采用12-十四酸佛波酯-13-乙酸盐(PMA)分别刺激体外培养的大鼠胶质瘤C6及NIH3T3细胞,在ZT0-ZT48之间设14个时间点,最初PMA的刺激时间记为ZT0,均在PMA刺激后每隔4h分别提取C6及NIH3T3细胞总RNA。利用标准曲线法Real-time PCR检测相应时间点Per2的mRNA的表达水平。结果 C6细胞中生物钟rPer2基因表达最高点在ZT28,最低点在ZT16; NIH3T3细胞中生物钟mPer2基因表达最高点在ZT24,最低点在ZT12,两者均呈现明显的节律性; C6细胞与NIH3T3细胞同时间点Per2基因表达差异均有统计学意义(均P<0.001)。结论 经PMA诱导后,Per2在C6及NIH3T3细胞均呈节律性表达,但二者表达节律存在明显差异,可作为研究生物种基因与胶质瘤的时间生物治疗的理论基础。
Abstract:
Objective To investigate the rhythm expression of circadian gene Period2(Per2)in glioma cells in vitro. Methods The cultured C6 glioma cells and NIH3T3 cells were stimulated by PMA. 14 time points were set between ZT0-ZT48 and initial PMA stimulation time was recorded as ZT0. Total RNA of C6 and NIH3T3 cells were extracted after PMA was stimulated every 4h. mRNA expression levels of the corresponding time points Per2 were detected with the Real-time PCR. Results The highest point of the biological clock rPer2 gene expression in C6 cells was ZT28 while the lowest point of the biological clock was ZT16; The highest point of the biological clock mPer2 gene expression in NIH3T3 cells was ZT24 while the lowest point of the biological clock was ZT12. Per2 gene expression showed significant difference in C6 cells and NIH3T3 cells at the same time point(all P<0.001). Conclusion PMA can induce the circadian oscillation of Per2 in both of C6 and NIH3T3 cells, but there are significant differences in the expression of rhythm. This can be used as theoretical basis of chronotherapy of gliomas.

参考文献/References:

[1] Aurelio Balsalobre,Francesca Damiola,and Ueli Schibler.A Serum Shock Induces Circadian Gene ExPressionin Mammalian Tissue Culture Cells[J].Cell,1998,93:929-937.
[2] Kentaro Oh-hashi,Yoshihisa Naruse,Masaki Tanaka.Intracellular calcium mobilization induces Period genes via MAP kinase Pathways in NIH3T3 cells[J].FEBS Letters,2002,516:101-105.
[3] Yong Zhu,Richard G,Stevens,et al.Testing the Circadian Gene HyPothesis in Prostate Cancer:A PoPulation-Based Case-Control Study[J].Cancer Res,2009,69:9315-9322.
[4] Zheng B,Albrecht U,Kaasik K,et al.Nonredundant roles of the mPer1 and mPer2 genes in the mammalian circadian clock[J].Cell,2001,105:683-694.
[5] 王正荣.时间生物学[M].北京:科学出版社,2006.
[6] Bae K,Jin X,Maywood ES, et al.Differential functions of mPer1,mPer2,and mPer3 in the SCN circadian clock[J].Neuron,2001,30:525-536.
[7] Schernhammer ES,Laden F,SPeizer FE,et al.Rotating night shifts and risk of breast cancer in women ParticiPating in the nurseshealth study[J].J Natl Cancer Inst,2001,93:1563-1568.
[8] HC Xia,ZF Niu,H Ma,et al.Deregulated exPression of the Per1 and Per2 in human gliomas[J].Can J Neurol Sci,2010,37(3):365-370.
[9] 邓香群,刘保安,杨军,等.钟基因hClock、hBmal1在结直肠肿瘤中的表达[J].肿瘤防治研究,2010,37(11):1288-1291.
[10] Aaron E,Hoffman,Tongzhang Zheng,et al.The Circadian Gene NPAS2,a Putative Tumor SuPPressor,Is Involved in DNA Damage Res,Ponse[J].Mol Cancer Res 2008,6(9):1461-1468.
[11] X Yang,PA Wood,CM Ansell,et al.The circadian clock gene Per1 suPPresses cancer cell Proliferation and tumor growth at sPecific times of day[J].Chronobiol Int,2009,26(7):1323-1339.
[12] Xiao-Mei Li,Franck Delaunay. Cancer Inhibition through Circadian ReProgramming of Tumor TranscriPtome with Meal Timing[J].Cancer Res,2010,70:3351-3360.
[13] Patricia A,Wood,Jovelyn Du-Quiton,et al.Circadian clock coordinates cancer cell cycle Progression,thymidylate synthase,and 5-fluorouracil theraPeutic index[J].Mol Cancer Ther,2006,5(8):2027-2030.
[14] Loning Fu,Helene Pelicano,Jinsong Liu,et al.The Circadian Gene Period2 PlaysanImPortant Role in Tumor SuPPression and DNA Damage ResPonse In Vivo[J].Cell,2002,111:41-50.

相似文献/References:

[1]王文斐,刘文庆,尚明华,等.胰岛素样生长因子-2在胶质瘤中表达的意义[J].宁夏医科大学学报,2014,(10):1076.
 WANG Wenfei,LIU Wenqing,SHANG Minghua,et al.The Expression of Insulin-like Growth Factor-2 in Tissues of Human Gliomas[J].Ningxia Medical University,2014,(05):1076.

备注/Memo

备注/Memo:
收稿日期:2012-10-27 基金项目:国家自然科学基金(30860289) 作者简介:李张珂(1986-),男,在读硕士研究生,主要从事胶质瘤生物种基因的研究。E-mail:lizhangke2008@163.com 通信作者:夏鹤春,主任医师。E-mail:xhechun@yahoo.com.cn
更新日期/Last Update: 2013-05-20