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[1]哈春芳,窦海丽,曹 丹,等.ADM低表达与月经过多及原因不明性不孕的关系[J].宁夏医科大学学报,2013,(04):365-367,385.
 HA Chun-fang,DOU Hai-li,CAO Dan,et al.The Relationship of Low ADM Expression with Menorrhagia and Infertility[J].Ningxia Medical University,2013,(04):365-367,385.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年04期
页码:
365-367,385
栏目:
论著
出版日期:
2013-04-20

文章信息/Info

Title:
The Relationship of Low ADM Expression with Menorrhagia and Infertility
作者:
哈春芳1 窦海丽2 曹 丹1 张 红1
1.宁夏医科大学总医院妇产科、宁夏医科大学生育力保持重点实验室,银川 750004; 2.宁夏银川市第四人民医院妇产科,银川 750021
Author(s):
HA Chun-fang1 DOU Hai-li2 CAO Dan1 ZHANG Hong1
1.Department of Gynecology, the General Hospital of Ningxia Medical University, Yinchuan 750004; 2.The Department of Gynecology in No. 4 Hospital of Ningxia, Yinchuan 750021
关键词:
肾上腺髓质素 降钙素受体相关受体 原因不明性不孕 月经过多 内膜血管疾病
Keywords:
adrenomedullin CRLR unexplained infertility menorrhagia vessel pathology
分类号:
R711.6
DOI:
-
文献标志码:
A
摘要:
目的 探讨肾上腺髓质素(adrenomedullin, ADM)及其相关受体降钙素受体相关受体(calcitonin relative receptor, CRLR)在月经过多及原因不明不孕发生中表达及其与内膜血管密度的关系。方法 免疫组化、荧光定量PCR法测定月经过多组妇女(n=20)、原因不明的不孕组妇女(n=22)黄体期子宫内膜中ADM、CRLR蛋白和基因表达、血管数目等的变化,选取健康妇女黄体期子宫内膜(n=20)做对照组。结果 免疫组化结果显示子宫内膜腺体中ADM蛋白的表达三组间差异均无统计学意义(P>0.05),而间质细胞中ADM表达不孕症组为(768±213.6),月经过多组为(907±286.6),均低于正常对照组(1243±342.8),P<0.001; 在腺上皮、腺体及间质中三组间CRLR蛋白表达差异均无统计学意义(P>0.05); QRT-PCR测定显示对照组内膜中的ADM mRNA表达为(4.5±0.70),较不孕症组(1.69±0.51)的高4.12倍,较月经过多组(0.73±0.56)的高5.6倍,而CRLR mRNA表达三组间比较差异无统计学意义; CD31标示的子宫内膜中微血管密度测定结果显示: 不孕症组内膜中微血管密度值(1.03±0.51)、微血管总数目(4.9±0.87)明显低于对照组(P<0.01); 月经过多组微血管密度(1.12±0.22)、总微血管数目(5.14±0.94)均低于对照组(P<0.01)。结论 原因不明性不孕及月经过多的患者发生与子宫内膜中ADM 蛋白和mRNA的呈低表达及血管密度的降低有关; 原因不明性不孕与月经过多可能同为低ADM表达所致的内膜血管异常性疾病。
Abstract:
Objective To investigate the different expression of ADMmRNA and CRLR both in endometrium of unexplained infertility and menorrhagia women,in order to demonstrate the relationship among them. Methods The endometrium from 22 unexpected infertile women, 20 menorrhagia women in luteal phase and 20 fertile women in luteal phase was studied with Immunohistrochemistry, RT-PCR and quantitative RT-PCR. The different expression of ADM and CRLR and vessels densinty in pratein and mRNA were analyse. Results The results of the immunohistochemistry indicated that the expression of ADM in glandular epithelium in infertiity group was no more than that in the menorrhagia group and in normal controls; The expression of ADM was appeared mainly in gland with no different staining in three groups(P>0.05); in the interstitial cells, the expression of ADM in infertiity group was(768±213.6),which was lower than that the menorrhagia group(907±286.6); both of them were lower than the normal controls(1243±342.8)(P<0.05). Also by this mean,there were no different in glandular epithelium and gland,including in the interstitial cell. The expression of CRLR.Q-PCR showed that the expression of ADM mRNA in the normal control's endometrium was(4.5±0.70),which was 4.12 times higher significantly than that in infertility(1.69±0.51), also 5.6 times higher significantly than that in menorrhagia group, while the expression of CRLR mRNA was same in three groups. The vessels densinty staining in infertile group was(1.03±0.51), and was(1.12±0.22)in menorrhagia, both of them were significantly lower than the value in fertile group(P<0.01). Conclusion The expression of ADM mRNA in the strome cells has correspondence with unexpected infertile and menorrhagia; Both unexpected infertility and menorrhagia may be the result of vessel pathology of the lower expression of ADM.

参考文献/References:

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[4] Chunfang HA.Anneli S-E Britt-Marle Landgren,et al.Adrenomedullin and its receptor, calcitonin receptor-like receptor,are aberrantly expressed in women with idiopathic menorrhagia[J]. Mol MED Reporte,2009,2: 7-11.
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相似文献/References:

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 WEI Shao-min,ZHANG Jian-zhong,AN Xin,et al.Protective Effect of Adrenomedullin on Heart Remodeling of SHR[J].Ningxia Medical University,2012,(04):456.

备注/Memo

备注/Memo:
收稿日期:2013-01-05 基金项目:国家自然基金项目(81160078) 作者简介:哈春芳,女,主任医师,博士,从事妇产科肿瘤和内分泌的基础和临床研究。
更新日期/Last Update: 2013-04-20