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[1]边 静,马 科,陈丽军,等.回药爱康方含药血清对人肺腺癌A549细胞生长增殖的影响[J].宁夏医科大学学报,2013,(04):0-360,364.
 BIAN Jing,MA Ke,CHEN Li-jun,et al.The Effect of Medicated Serum with Hui Medicine AiKang Recipe on Growth and Proliferation of Human Lung Adenocarcinoma A549 Cells[J].Ningxia Medical University,2013,(04):0-360,364.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年04期
页码:
0-360,364
栏目:
回医药学
出版日期:
2013-04-20

文章信息/Info

Title:
The Effect of Medicated Serum with Hui Medicine AiKang Recipe on Growth and Proliferation of Human Lung Adenocarcinoma A549 Cells
作者:
边 静1 马 科1 陈丽军2 周 慧1 武 震1 马治国3 周丽萍2
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院,银川 750004; 3.宁夏医科大学附属银川市中医医院,银川 750001
Author(s):
BIAN Jing1 MA Ke1 CHEN Li-jun3 ZHOU Hui1 WU Zhen1 MA Zhi-guo3 ZHOU Li-ping2
1.Ningxia Medical University, 750004; 2.Hospital of Traditional Chinese Medicine of Yinchuan City, 750001; 3.Ningxia Medical University Affiliated Hospital, 750001
关键词:
回药爱康方 含药血清 A549细胞 生长增殖
Keywords:
Hui Aikang Fang medicated serum A549 cells proliferation
分类号:
R734.2
DOI:
-
文献标志码:
A
摘要:
目的 探讨回药爱康方含药血清对人肺腺癌A549细胞生长的抑制作用及对细胞形态的影响。方法 采用血清药理学方法,将SD大鼠分为对照组、爱康方低、中、高剂量组、环磷酰胺化疗组、回药爱康方低、中、高剂量分别联合环磷酰胺化疗组,制备含药血清; 培养人肺腺癌A549细胞,将细胞分为实验组和对照组,实验组分别给予含5%、10%、20%浓度的相应各组含药血清,对照组给予等浓度的对照组大鼠血清,培养24、48、72h后观察细胞形态变化,采用MTT法测定各组含药血清对A549细胞的生长抑制率。结果 光镜下观察到,与对照组相比,各剂量回药爱康方含药血清可使A549细胞有不同程度的胞膜回缩、胞核固缩、细胞间距增大和染色质边缘化现象,其中以爱康方高剂量联合环磷酰胺组作用72h最明显。MTT法检测细胞生长抑制率发现,各实验组各浓度抑制率与相应对照组比较,实验组72h抑制率均高于对照组(P<0.05); 单纯环磷酰胺72h抑制率均高于爱康方低、中、高剂量(P<0.05); 爱康方高剂量联合环磷酰胺72h抑制率高于爱康方低剂量联合环磷酰胺和单纯环磷酰胺(P<0.05)。结论 回药爱康方可有效抑制A549细胞生长增殖,并增强环磷酰胺对A549细胞的抑制作用。
Abstract:
Objective To observe the effect of medicated serum with Hui Aikang Fang on growth and proliferation of human lung adenocarcinoma A549 cells. Methods Using the serum pharmacology, SD rats were divided into low, medium, high dose Aikang Fang group, Cyclophosphamide(CTX)group, low, medium, high dose Aikang Fang combined with CTX group and normal saline group. The medicated serum with different concentrations of Aikang Fang were prepared. The cells were divided into experimental and control groups: the experimental group were treated with 5%, 10%, 20% concentration corresponding group medicated serum while control group was given the same concentration of control serum.The morphological changes of the cells were observed at 24h, 48h, 72h in each group. The proliferation of A549 was measured by methyl thiazolyl tetrazolium(MTT)method. Results Observed under the microscope and compared with the control group, the distance between the cells were increased, nuclear condensation, the chromatin concentrated to edge in Aikang Fang groups. By MTT method, compared with control group, all medicated serum groups' inhibition rates were significantly higher at 72 hours(P<0.05). The inhibition rate of CTX group were significantly higher than that of low, middle, high dose of Aikang Fang group at 72 hours(P<0.05). The inhibition rate of high dose Aikang Fang combined with CTX were significantly higher than that of low dose Aikang Fang combined with CTX and CTX at 72 hours(P<0.05). Conclusion Hui Aikang Fang can inhibit proliferation and strengthen the curative effect of CTX on A549 cells.

参考文献/References:

[1] Ferlay J, Shin HR, Bray F, et al. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008[J]. International journal of cancer, 2010,127(12):2893-2917.
[2] Jemal A, Bray F, Center MM, et al. Global cancer statistics[J]. CA: a cancer journal for clinicians, 2011,61(2):69-90.
[3] Organization WH. WHO Global Health Observatory(GHO)[Z]. 2010.
[4] Nguyen KH, Kobayashi S, Costa DB. Acquired Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Cancers Dependent on the Epidermal Growth Factor Receptor Pathway[J]. Clinical lung cancer, 2009,10(4):281-289.
[5] 曹向明, 沈伟生, 舒中琴, 等. 薏苡仁甘油三酯在吉西他滨三维适形同步放化疗治疗局部晚期胰腺癌中免疫调节功能观察[J]. 南京医科大学学报:自然科学版, 2012(10):1414-1416.
[6] 郭莉婷, 程月新, 姜藻. 胃癌中B7-H4和B7-H3的表达及薏苡仁酯对表达的影响[J]. 现代肿瘤医学, 2011(9):1791-1795.
[7] 陈兵, 李翠萍, 欧阳建, 等. 通关藤提取物体外对人正常免疫细胞及造血干细胞的影响[J]. 临床肿瘤学杂志, 2010,15(10):887-890.
[8] 赓迪, 袁媛, 王春梅. 云南琵琶甲提取物抑制 Hela 细胞增殖和诱导凋亡的研究[A]. 2007 年全国生化与生物技术药物学术年会论文集[C], 2007:310-313.
[9] 马科,马立凤,牛阳,等.回药爱康方治疗中晚期原发性肺癌 30 例效果评价[J].宁夏医科大学学报,2009,31(2):256-258.
[10] 马治国, 周丽萍, 边静,等.HE法观察回药爱康方对小鼠Lewis肺癌的抑制作用[J]. 辽宁中医杂志,2012,(2): 364-366.

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备注/Memo

备注/Memo:
收稿日期:2013-01-26 基金项目:国家自然科学基金项目(81160462) 作者简介:边静(1987-),女,在读硕士研究生,研究方向:回药、中药防治恶性肿瘤的实验研究。 通信作者:马科(1973-),男(回族),教授,硕士研究生导师,博士。E-mail:osmnmake@126.com
更新日期/Last Update: 2013-04-20