|本期目录/Table of Contents|

[1]赵 琳,景 丽,郭凤英,等.TGF-β和ERK1/2在自发性高血压大鼠主动脉中的表达[J].宁夏医科大学学报,2012,(10):996-999.
 ZHAO Lin,JING Li,GUO Feng-ying,et al.Expression of Transforming Growth Factor-beta 1 and Extracellular Signal Regulated Kinase1/2 in the Aorta of Spontaneously Hypertensive Rats[J].Ningxia Medical University,2012,(10):996-999.
点击复制

TGF-β和ERK1/2在自发性高血压大鼠主动脉中的表达(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年10期
页码:
996-999
栏目:
论著
出版日期:
2012-10-30

文章信息/Info

Title:
Expression of Transforming Growth Factor-beta 1 and Extracellular Signal Regulated Kinase1/2 in the Aorta of Spontaneously Hypertensive Rats
作者:
赵 琳12 景 丽1 郭凤英1 张建中1
1.宁夏医科大学基础医学院病理系,银川 750004; 2.西安交通大学医学院,西安 710061
Author(s):
ZHAO Lin12 JING Li1 GUO Feng-ying1 ZHANG Jian-zhong1
1.Dept. of Pathology Ningxia Med. Univ.,Yinchuan 750004; 2.Xi'an Jiaotong Univ. College of Medicine,Xi'an 710061
关键词:
高血压 血管平滑肌细胞 血管内皮细胞 细胞外信号调节激酶 转化生长因子-β 自发性高血压大鼠
Keywords:
hypertension vascular smooth muscle cell endothelial cell transforming growth factor-β1 extracellular signal-regulated kinase1/2 spontaneously hypertensive rats
分类号:
R541.3; Q753
DOI:
-
文献标志码:
A
摘要:
目的 探讨细胞外信号调节激酶(ERK1/2)和转化生长因子-β(TGF-β1)在自发性高血压大鼠(SHR)主动脉血管平滑肌细胞(VSMC)和血管内皮细胞(EC)中的表达及其意义。方法 将SHR分为4、8、16和20周龄组,采用免疫组化和Western blot 方法检测主动脉血管EC和VSMC中ERK1/2和TGF-β1蛋白的表达。结果 SHR从8周龄开始血压升高,20周龄组收缩压达到(180±21)mmHg; Western blot 检测显示,ERK1/2和TGF-β1在SHR16和SHR20周龄组VSMC和EC中的表达与4周龄组相比增高(P<0.05),且ERK1/2和TGF-β1 蛋白积分光密度值与血压呈正相关; ERK1/2免疫组化结果显示,在SHR16和20周龄组,主动脉中内皮细胞阳性率分别为72.69%和70.14%、VSMC阳性率分别为35.01%和28.54%,明显高于SHR4周龄组(P<0.01); 在SHR主动脉中,TGF-β1免疫组化表达均为阴性。结论 在SHR动脉血管平滑肌细胞和内皮细胞中ERK1/2和TGF-β1的表达随着血压的增高呈现增加的趋势,推测ERK1/2和TGF-β1相互共同作用参与主动脉血管改变。
Abstract:
Objective To explore the expression and significance of extracellular signal-regulated kinase1/2,transforming growth factor-β1 in the VSMC and EC of spontaneously hypertensive rats(SHR). Methods SHR were divided into 4 groups according to their ages(4-week old, 8-week old, 16-week old and 20-week old). The expression of ERK1/2, TGF-β1 in the aorta of the 4 groups were tested by western blot and immunohistochemistry. Results SHRs' systolic blood pressure significantly increased since 8 weeks and sysotic blood pressure was(180±21)mmHg in 20 weeks old SHR.Western blot assays showed that ERK1/2 and TGF-β1 in EC and VSMC of SHR16 and SHR20 were significantly higher than those in SHR4(P<0.05). A positive correlation was found between the optical density of ERK1/2 and TGF-β1 and the blood pressure. Immunohistochemistry assays showed that the positive rate of ERK1/2 in EC of SHR16 and SHR20 was 72.69% and 70.14% respectively, and in VSMC was 35.01% and 28.54%,both of which were higher than those of SHR4(P<0.05).The expression of TGF-β1 were all negative in aorta of SHR. Conclusion For SHR, the level of the expression of ERK1/2 and TGFβ1 increases along with the blood pressure level. It is extrapolated that ERK1/2 and TGF-β1 participated in aorta lesion.

参考文献/References:

[1] Dzau VJ, Gibbons GH,Morishita R, et al. New perspectives in hypertension research. Potentials of vascular biology[J]. Hypertension,1994,23(6pt2):1132-1140.
[2] Souad Belmadani, Mourad Zerfaoui,Hamid A. Boulares, et al. Microvessel vascular smooth muscle cells contribute to collagen type I deposition through ERK1/2 MAP kinase, αvβ3 –integrin, and TGFβ-1 in response to ANG II and high glucose[J] Am J Physiol Heart Circ Physiol, 2008,295: H69–H76.
[3] Nerea Hermida, Begon a Lo'pez, Arantxa Gonza'lez,et al. A synthetic peptide from transformi ng growth factor-b1 type III receptor prevents myocardial fibrosis in spontaneously hypertensive rats [J].Cardiovascular Research,2009, 81:601–609.
[4] Yurovsky VV, Gerzanich V, Ivanova S, et al. Autocrine TGF-beta1 mediates angiotensin II-induced proliferative response of cerebral vessels in vivo[J].Am J Hypertens, 2007,20: 950–956.
[5] D Duguay and D deBlois. Differential regulation of Akt, caspases and MAPkinases underlies smooth muscle cell apoptosis during aortic remodeling in SHR treated with amlodipine[J].British Journal of Pharmacology,2007, 151:1315–1323.
[6] Maria Arejian, Yuan Li, and Madhu B,et al. Nitric oxide attenuates the expression of natriuretic peptide receptor C and associated adenylyl cyclase signaling in aortic vascular smooth muscle cells: role of MAPK[J]. Am J Physiol Heart Circ Physiol, 2009,296: H1859–H1867.
[7] Nishi NS, Dipak PR.The role of transforming growth factor b in atherosclerosis [J].Cytokine& Growth FactorReviews, 2006, 17: 487-499.
[8] Annette S, Sven G, Wolfgang V,et al. Exogenous nitric oxide causes overexpression of TGF—β1 and overproduction of extracellular matrix in human coronary smooth muscle cells[J]. Cardiovascular Research, 2003:58(3):671-678.
[9] Wen-Han Cheng, Pei-Jung Lu, Wen-Yu Ho,et al. Angiotensin II Inhibits Neuronal Nitric Oxide Synthase Activation Through the ERK1/2-RSK Signaling Pathway to Modulate Central Control of Blood Pressure[J].Circulation Research,2010, 106: 788- 795.
[10] Takuya Kishi, Yoshitaka Hirooka, Satomi Konno,et al. Angiotensin II Type Receptor–Activated Caspase-3 Through Ras/Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase in the Rostral Ventrolateral Medulla Is Involved in Sympathoexcitation in Stroke-Prone Spontaneously Hypertensive Rats [J]. Hypertension,2010, 55:291-297.
[11] Aaron B,Baker, David S. Ettenson, Michael Jonas,et al. Endothelial Cells Provide Feedback Control for Vascular Remodeling Through a Mechanosensitive Autocrine TGF-β Signaling Path way [J].Circulation Research,2008,103:289-297.
[12] Jing L, Zhang JZ, Zhao L, et al. High-expression of transforming growth factor β1 and phosphorylation of extracellular signal-regulated protein kinase in vascular smooth muscle cells from aorta and renal arterioles of spontaneous hypertension rats [J]. Clin Exp Hypertens, 2007, 29(2):107-117.

相似文献/References:

[1]邓瀚.妊娠期高血压危险因素及其对妊娠结局的影响[J].宁夏医科大学学报,2013,(08):931.
[2]黄占淑,吴荣.舒血宁注射液治疗高血压性脑出血恢复期早期52例疗效观察[J].宁夏医科大学学报,2013,(06):712.
[3]路静,杨玲娣,张建中,等.N-ras在自发性高血压和两肾一夹型高血压大鼠肾脏动脉丛表达的比较研究[J].宁夏医科大学学报,2013,(06):613.
 LU Jing,YANG Ling-di,ZHANG Jian-zhong,et al.[J].Ningxia Medical University,2013,(10):613.
[4]刘秀英,马 英,游淑华,等.银川市社区1737例高血压患者多代谢异常的流行病学特征分析[J].宁夏医科大学学报,2013,(04):428.
 LIU Xiu-ying,MA Ying,YOU Shu-hua,et al.The Epidemiological Characteristics of Multiple Metabolic Disorders in 1737 Patients with Hypertension in Yinchuan Communities[J].Ningxia Medical University,2013,(10):428.
[5]张银娥,王晓莉,杨 艺,等.社区高血压患者综合干预效果评价[J].宁夏医科大学学报,2013,(04):407.
 ZHANG Yin-e,WANG Xiao-li,YANY Yi,et al.Evaluation of a Community-based Integrated Intervention in Patients with Hypertension[J].Ningxia Medical University,2013,(10):407.
[6]王 露,金梅花,徐清斌,等.原发性高血压患者血管紧张素Ⅱ-2型受体基因C3123A 多态性及其相关因素的研究[J].宁夏医科大学学报,2013,(04):382.
 WANG Lu,JIN Mei-hua,XU Qing-bin,et al.Relationship between C3123A Polymorphism of AngiogenesisⅡ Type 2 Receptor Gene and Essential Hypertension of Hans in Ningxia[J].Ningxia Medical University,2013,(10):382.
[7]赵艳姣,刘新兰,马志兰.VEGF-C、VEGFR-3和LYVE-1在胃癌中的表达及其临床意义[J].宁夏医科大学学报,2013,(04):378.
 ZHAO Yan-jiao,LIU Xin-lan,MA Zhi-lan.Expression and Clinical Significance of VEGF-C, VEGFR-3 and LYVE-1 in Gastric Cancer[J].Ningxia Medical University,2013,(10):378.
[8]赵兴,郭健伟,刘欢,等.原发性高血压患者红细胞和血小板参数的变化与颈动脉粥样硬化的关系[J].宁夏医科大学学报,2013,(02):206.
[9]赵金鑫,张 琳,宁艳花.高血压患者社区综合干预效果评价[J].宁夏医科大学学报,2013,(02):134.
 ZHAO Jin-xin,ZHANG Lin,NING Yan-hua.Evaluation on Effect of Comprehensive Community-based Intervention of Hypertension[J].Ningxia Medical University,2013,(10):134.
[10]许红霞,张毓洪,刘 瑛,等.银川市农村青年人高血压患病率及危险因素分析[J].宁夏医科大学学报,2013,(01):63.
 XU Hong-xia,ZHANG Yu-hong,LIU Ying,et al.Prevalence and Risk Factors of Hypertension among Rural Young Adults in Yinchuan City[J].Ningxia Medical University,2013,(10):63.

备注/Memo

备注/Memo:
收稿日期:2012-01-31 作者简介:赵琳,女,在读博士研究生,讲师,研究方向:心血管病理学。E-mail:zhaolincn@sina.com 通信作者:张建中,男,教授,研究方向:心血管病理学。 E-mail:zhangjz@nxnu.edu.cn
更新日期/Last Update: 2012-10-20