|本期目录/Table of Contents|

[1]黄 艳,吴兴临,张建中,等.糖尿病大鼠脑内微血管细胞外信号调节激酶磷酸化的研究[J].宁夏医科大学学报,2012,(09):881-883,886.
 HUANG Yan,WU Xing-lin,ZHANG Jian-zhong,et al.The Phosphorylation of Extracellular Signal-regulated Kinase in the Microvascular in Rats with Diabetes[J].Ningxia Medical University,2012,(09):881-883,886.
点击复制

糖尿病大鼠脑内微血管细胞外信号调节激酶磷酸化的研究(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年09期
页码:
881-883,886
栏目:
论著
出版日期:
2012-09-30

文章信息/Info

Title:
The Phosphorylation of Extracellular Signal-regulated Kinase in the Microvascular in Rats with Diabetes
作者:
黄 艳1 吴兴临1 张建中2 王 岩1
1.宁夏银川市第一人民医院,银川 750001; 2.宁夏医科大学总医院病理科,银川 750004
Author(s):
HUANG Yan1 WU Xing-lin1 ZHANG Jian-zhong2 WANG Yan1
1.The First People Hospital of Yinchuan,Yinchuan 750004; 2.Department of Pathology, General Hospital of Ningxia Medical University,Yinchuan 750004
关键词:
糖尿病大鼠 细胞外信号调节激酶 ets样基因1 微血管 血管内皮细胞 血管平滑肌细胞
Keywords:
diabetes mellitus extracellular signal-regulated kinase ets-like gene 1 brain microvascular vascular endothelial cell vascular smooth muscle cell
分类号:
R587
DOI:
-
文献标志码:
A
摘要:
目的 研究链脲佐菌素诱导的Ⅰ型糖尿病大鼠脑膜、脑实质微血管的病理改变及其中细胞外信号调节激酶ERK1/2及其下游作用底物转录因子Elk-1的磷酸化状态,探讨糖尿病血管的发病机制。方法 应用链脲佐菌素制备Ⅰ型糖尿病大鼠模型,HE染色观察制模后3d和2、4、8周脑膜、脑实质微血管病变,免疫组织化学观察高血糖状态下脑内微血管中p-ERK1/2 及下游作用底物p-ELK1的表达情况。结果 p-ERK1/2、p-ELK1在各时间点正常对照组和糖尿病组大鼠脑膜、脑实质微血管中均有不同程度的表达。p-ERK1/2在糖尿病4、8周组脑膜、脑实质微血管中表达均明显上调; p-ELK1在糖尿病4、8周组脑膜、脑实质微血管内皮细胞中表达均明显的上调(P<0.05),分别与糖尿病3d、2周组及各期正常对照组比较差异有统计学意义(P<0.01)。糖尿病各组与正常对照组各组脑膜微血管平滑肌细胞中p-ELK1的表达差异无统计学意义(P>0.05)。结论 在糖尿病4、8周组SD大鼠脑内微血管中存在ERK1/2信号转导通路的异常激活。
Abstract:
Objective To investigate the phosphorylation of extracellular signal-regulated kinase(ERK1/2)and ets like gene-1(ELK-1)in meningeal and brain microvascular endothelial cells and smooth muscle cells with diabetic rats and to explore the mechanism of diabetic brain microangiopathy. Methods After modelling 0.5 weeks, two weeks, four weeks, eight weeks, the spleen central artery angiopathy of Type I diabetic rats,which was induced using intraperitoneal injection of STZ, was respectively observated. The phosphorylation of extracellular signal-regulated kinase(ERK1/2)and ets like gene-1(ELK-1)in the brain microvascular were detected by means of HE staining,and immunohistochemistry techniques. Results p-ERK,p-ELK expressed with different levels in meningeal microvascular endothelial cells, smooth muscle cells, brain microvascular endothelial cells of diabetic groups and normal control group. The up-regulation of phosphorylation ERK1/2 was found in meningeal microvascular endothelial cells, smooth muscle cells, brain microvascular endothelial cells of 8 week,4 week diabetic group. The up-regulation of phosphorylation Elk-1 expression was found in meningeal and brain microvascular endothelial cells of 8 week, 4 week diabetic group. But there were no significant difference in meningeal microvascular smooth muscle cells between diabetes groups and the normal control groups(P>0.05). Conclusion In 8 week, 4 week diabetic meningeal and brain microvascular exist ERK1 / 2 signal transduction pathway activation.

参考文献/References:

[1] 纪立农. 糖尿病患者的降压治疗与心血管保护[J]. 中华高血压杂志, 2010, 18(5): 414-416.
[2] 徐勇,黄颂敏. MAPK家族与糖尿病并发症[J]. 国外医学:内分泌学分册, 2001, 21(1): 8-10.
[3] ZetterBR. The endothelial cells of large and small blood vessels[J]. Diabetes, 1981, 30(Suppl 2):24-28.
[4] Glaros EN,Kim WS,Gamer B. Myriocin-mediated up-regulation of hepatocyte apoA-I synthesis is associated with ERK inhibition[J]. Clin Sci( Lond), 2010, 118(12): 727-736.
[5] McCubrey JA,Steelman LS,Chappell WH, et al. Roles of the Raf /MEK/ERK pathway in cell growth, malignant, transformation and drug resistance[J]. Biochim Biophys Acta, 2007, 1773(8): 1263-1284.
[6] 黄艳,景丽,张建中,等. 糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨[J]. 宁夏医学院学报, 2008, 30(2): 143-145.

相似文献/References:

[1]王 铎,王 虎,马巧丽.脑梗死患者雌激素水平与阿司匹林抵抗的关系[J].宁夏医科大学学报,2012,(10):1016.
 WANG Duo,WANG Hu,MA Qiao-li.The Relationship between Estrogen and Cerebral Infarction[J].Ningxia Medical University,2012,(09):1016.
[2]赵 琳,景 丽,郭凤英,等.TGF-β和ERK1/2在自发性高血压大鼠主动脉中的表达[J].宁夏医科大学学报,2012,(10):996.
 ZHAO Lin,JING Li,GUO Feng-ying,et al.Expression of Transforming Growth Factor-beta 1 and Extracellular Signal Regulated Kinase1/2 in the Aorta of Spontaneously Hypertensive Rats[J].Ningxia Medical University,2012,(09):996.
[3]赵 琳,景 丽,张建中,等.VEGF和ERK1/2在不同周龄自发性高血压大鼠肾脏中的表达[J].宁夏医科大学学报,2012,(08):771.
 ZHAO Lin,JING Li,ZHANG Jian-zhong,et al.Expression of VEGF and ERK1/2 in Spontaneously Hypertensive Rats Kidney at Different Weeks of Ages[J].Ningxia Medical University,2012,(09):771.
[4]杨振兴,李宗正.小剂量地塞米松对创伤性脑水肿AQP4的影响[J].宁夏医科大学学报,2012,(06):600.
 YANG Zhen-xing,LI Zong-zheng.Influence of Small Dose of Dexamethasone on AQP4 in Rats with Brain Trauma[J].Ningxia Medical University,2012,(09):600.
[5]于海娇,徐支芳,马琳娜,等.同型半胱氨酸对血管平滑肌细胞增殖 和细胞周期的影响及叶酸的拮抗作用[J].宁夏医科大学学报,2012,(01):7.
 YU Hai-jiao,XU Zhi-fang,MA Lin-na,et al.The Effect of Homocysteine on the Proliferation of Vascular Smooth Muscle Cells in Vitro and the Antagonizing Effect of Folic Acid[J].Ningxia Medical University,2012,(09):7.
[6]韩金利,刘广辉,刘海洋,等.黄芪对大鼠脑出血灶周围神经元Caspase-3表达的影响[J].宁夏医科大学学报,2013,(05):489.
 HAN Jin-li,LIU Guang-hui,LIU Hai-yang,et al.Effect of the Astragalus on Caspase-3 Expression of Neurons Around Intracerebral Hemorrhage in Rats[J].Ningxia Medical University,2013,(09):489.
[7]赵艳姣,刘新兰,马志兰.VEGF-C、VEGFR-3和LYVE-1在胃癌中的表达及其临床意义[J].宁夏医科大学学报,2013,(04):378.
 ZHAO Yan-jiao,LIU Xin-lan,MA Zhi-lan.Expression and Clinical Significance of VEGF-C, VEGFR-3 and LYVE-1 in Gastric Cancer[J].Ningxia Medical University,2013,(09):378.
[8]张永梅,陈桂生,戴秀英,等.宁夏农村癫痫患者脑电图检查结果分析[J].宁夏医科大学学报,2013,(03):289.
 ZHANG Yong-mei,CHEN Gui-sheng,DAI Xiu-ying,et al.The Characteristics of ECG in Patients with Epilepsy in Ningxia Rural Areas[J].Ningxia Medical University,2013,(09):289.
[9]涂小平,郑传丽,许贤瑞.复方丹参滴丸在脑梗死阿司匹林抵抗患者中的应用研究[J].宁夏医科大学学报,2013,(02):172.
 TU Xiao-ping,ZHENG Chuan-li,XU Xian-rui.Application of Compound Danshen Dropping Pills in Cerebral Infarct Patients with Aspirin Resistance[J].Ningxia Medical University,2013,(09):172.
[10]摆 茹,张 快,王 洁,等.氧化槐定碱对小鼠局灶性脑缺血再灌注损伤后NO、NOS变化的影响[J].宁夏医科大学学报,2013,(09):968.
 BAI Ru,ZHANG Kuai,WANG Jie,et al.Effects of Oxysophoridine on the Level of NO and NOS in Brain Tissue of Mice with Cerebral Ischemia and Reperfusion Injury[J].Ningxia Medical University,2013,(09):968.

备注/Memo

备注/Memo:
收稿日期:2011-12-09 作者简介:黄艳(1982-),主治医师,硕士,从事心脑血管病理学研究工作。 通信作者:张建中,博士生导师.zhangjz@nxmc.edu.cn
更新日期/Last Update: 2012-09-20