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[1]梁 宇,廖国玲,马琳娜,等.小鼠组织SAM和SAH在动脉粥样硬化评价中的应用[J].宁夏医科大学学报,2012,(09):872-875.
 LIANG Yu,LIAO Guo-ling,MA Lin-na,et al.The Application of SAM and SAH in Mice Tissues in Evaluation of Atherosclerosis[J].Ningxia Medical University,2012,(09):872-875.
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小鼠组织SAM和SAH在动脉粥样硬化评价中的应用(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年09期
页码:
872-875
栏目:
论著
出版日期:
2012-09-30

文章信息/Info

Title:
The Application of SAM and SAH in Mice Tissues in Evaluation of Atherosclerosis
作者:
梁 宇 廖国玲 马琳娜 曹成建 姜怡邓
宁夏医科大学检验学院,银川 750004
Author(s):
LIANG Yu LIAO Guo-ling MA Lin-na CAO Cheng-jian JIANG Yi-deng
Medical Test College of Ningxia Medical University, Yinchuan 750004
关键词:
S-腺苷同型半胱氨酸 S-腺苷甲硫氨酸 蛋氨酸 ApoE-/-小鼠
Keywords:
S-adenosyl homocysteine S-adenosylmethionine methionine ApoE-/- mice
分类号:
R541.4
DOI:
-
文献标志码:
A
摘要:
目的 探讨S-腺苷同型半胱氨酸(S-adenosyl Homocysteine, SAH)和S-腺苷甲硫氨酸(S-Adenosylmethionine, SAM)在载脂蛋白E基因敲除(ApoE-/-)小鼠动脉粥样硬化(Atherosclerosis, AS)评价中的应用。方法 采用高效液相色谱法(High Performance Liquid Chromatography, HPLC)检测各组ApoE-/-小鼠(模型对照组、高蛋氨酸饮食组、叶酸和维生素B12治疗组)心脏、肝脏、肾脏组织中SAM和SAH含量的变化,并与正常对照组比较。HE染色光镜观察各组主动脉病理学改变。结果 HPLC实验数据表明,正常对照组、模型对照组、高蛋氨酸饮食组的小鼠心脏、肝脏、肾脏组织中SAM、SAH的含量依次增加,治疗组小鼠心脏、肝脏、肾脏组织中SAM、SAH的含量则有所降低,与正常对照组比较差异均有统计学意义(P<0.05)。与模型对照组比较,肾脏高蛋氨酸饮食组SAM、SAH含量差异有统计学意义(P<0.05),治疗组肝脏、肾脏SAM、SAH含量差异有统计学意义(P<0.01)。与高蛋氨酸饮食组比较,肝脏、肾脏治疗组SAM、SAH含量有明显差异(P<0.01)。同时,HE染色显示高蛋氨酸饮食组主动脉AS的病理改变明显。结论 SAM、SAH含量升高可能是高蛋氨酸饮食促进ApoE-/-小鼠动脉粥样硬化发生的重要机制之一。
Abstract:
Objective Using high performance liquid chromatography(HPLC)to detect the changes of S-adenosyl-homocysteine(SAH)and S-adenosyl methionine(SAM), and to investigate its application in the evaluation of atherosclerosis(AS)in apolipoprotein E-knockout(ApoE-/-)mice. Methods SAM and SAH content changes in the hearts, livers and kidneys tissues of ApoE-/- mice groups(model control group, high-methionine diet group, folic acid and vitamin B12 treatment group)were detected and compared. Pathological changes of aorta in each group were observed by HE staining with light microscope. Results The results of HPLC showed that SAM, SAH levels in the normal control group, model control group and high-methionine diet group mice's hearts, livers and kidneys tissues were increased gradually. The SAM and SAH levels in the treatment group of ApoE-/- mice's hearts, livers and kidneys tissues were decreased. There were significant differences compared with the normal control group(P<0.05). Compared with the model group, the differences of SAM and SAH contents in kidneys of high methionine diet group were significant(P<0.05), the contents of SAM and SAH in livers and kidneys of treatment group were significantly different(P<0.01). Compared with the high methionine diet group, there were significant difference of the SAM and SAH levels in livers, kidneys of treatment group(P<0.01). Meanwhile, HE staining showed that AS pathological changes of aorta were significant in high-methionine diet group. Conclusion The increased expression of SAM, SAH may be one of the mechanisms that promoting the occurrence of atherosclerosis in the high-methionine diet ApoE-/- mice.

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备注/Memo

备注/Memo:
收稿日期:2012-01-17 基金项目:2010国家自然科学基金(30960124); 2010年教育部新世纪优秀人才支持计划(NCET-10-0916); 2011国家自然科学基金(81160044) 作者简介:梁宇(1986-),男,在读硕士研究生,从事动脉粥样硬化分子生物学研究。 通信作者:姜怡邓(1974-),男,副教授,硕士研究生导师,博士,从事动脉粥样硬化分子机制的研究。E-mail: jwcjyd@163. com
更新日期/Last Update: 2012-09-20