|本期目录/Table of Contents|

[1]张晓光,张庭元,杨 旭,等.黄体生成素干预对体外培养小鼠卵巢VEGF和Survivin表达的影响[J].宁夏医科大学学报,2012,(07):663-665,672.
 ZHANG Xiao-guang,ZHANG Ting-yuan,YANG xu,et al.Effects of LH Intervention on the Expressions of Cultured Mouse Ovarian VEGF and Survivin[J].Ningxia Medical University,2012,(07):663-665,672.
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黄体生成素干预对体外培养小鼠卵巢VEGF和Survivin表达的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年07期
页码:
663-665,672
栏目:
论著
出版日期:
2012-07-30

文章信息/Info

Title:
Effects of LH Intervention on the Expressions of Cultured Mouse Ovarian VEGF and Survivin
作者:
张晓光12 张庭元12 杨 旭12 陈 杰12 王燕蓉12 裴秀英13
1.宁夏医科大学生育力保持省部共建教育部重点实验室、宁夏生殖与遗传重点实验室,银川 750004; 2.宁夏医科大学基础医学院人体解剖与组织胚胎学系,银川 750004; 3.宁夏医科大学基础医学院 生物化学与分子生物学系,银川 750004
Author(s):
ZHANG Xiao-guang12 ZHANG Ting-yuan12 YANG xu12CHEN Jie12 WANG Yan-rong12 PEI Xiu-ying13
1.Key Laboratory of Fertility Maintenance, Ministry of Education, Ningxia Medical University & Key Laboratory of Reproduction and Genetics,Yinchuan 750004; 2. Faculty of Human Anatomy and Histoembrylolgy, Ningxia Med. Univ.,Yinchuan 750004; 3.Facul
关键词:
黄体生成素 存活素 血管内皮生长因子 小鼠卵巢 体外培养
Keywords:
LH survivin VEGF ovary of mouse in vitro culture
分类号:
R321
DOI:
-
文献标志码:
A
摘要:
目的 探索黄体生成素(LH)对小鼠卵巢体外培养后血管内皮生长因子(VEGF)和存活素(Survivin)表达的影响。方法 将从8周龄小鼠新鲜取材的半卵巢分别在无LH(培养对照组)和添加LH(0.15IU·mL-1)干预下培养3、6、12、24h后通过免疫组化法观察半卵巢VEGF、Survivin的表达。结果 培养组VEGF、Survivin表达比新鲜对照组降低且呈逐渐降低趋势; 在培养3、6、12h阶段,LH 0.15干预后VEGF阳性表达高于培养对照组(P<0.05 ); 3h和12h Survivin的表达均高于培养对照组(P<0.05 )。结论 LH体外干预可上调小鼠卵巢的VEGF、Survivin表达,VEGF和Survivin表达呈正相关。
Abstract:
Objective To explore the effects of the intervention of luteinizing hormone(LH)on the expression of ovarian angiogenic factors of VEGF and Survivin in cultured mouse ovarians in vitro. Methods The expression of VEGF and Survivin were monitored by immunohistochemical method after the hemi-ovaries of juvenescent mice had been cultured in the culturing mediums that contain LH of 0.00IU·mL-1 and 0.15IU·mL-1 for 3, 6, 12,and 24hours. Results The expression of VEGF and Survivin in culture group was significantly lower than that in fresh control group. The expression of VEGF in LH group was higher than that in non-LH group at 3h,6h,12h(P<0.05). The expression of Survivin in LH group was higher than that in non-LH group at 3h, 12h.(P<0.05). Conclusion LH intervention in vitro can increase expression of VEGF and Survivin in cultured mouse ovarians in vitro.

参考文献/References:

[1] Aubard Y,Piver P,Cogni Y,et al. Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep[J]. Hum Reprod,1999,14(8):2149-2154.
[2] 张皓云,管英俊,孙秀宁.冻存卵巢移植后功能及其影响因素[J].解剖科学进展,2004,10(4):332-335.
[3] Wang, Y.Chang, Q.Sun, J. et al. Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue[J]. Reproductive Biomedicine Online,2012, 24:646-653.
[4] Kusumanto YH, Weel VV, Mulder NH, et al.Treatment with intramuscular vascular endothelial growth factor gene compared with placebo for patients with diabetes mellitus and critical limb ischemia:a double-blind randomized trial[J].Hum Gen Ther,2006,17(6):683-691.
[5] Connor D, S.Schechner J, S.Adida C,et al. Control of apoptosis during angiogenesis by survivin expression in endothelial cells[J]. Am J Pathol,2000, 156(2):393-398.
[6] Brock TA,Dvorak HF,Senger DR. Tumor-secreted vascular permeability factor increases cytosolic Ca2+ and von Willebrand factor release in human endothelial cells[J]. Am J Pathol,1991,138(1):213-221.
[7] Shin S,Sung B,J.Cho YS, et al. An anti-apoptotic protein human survivin is a direct inhibitor of caspase-3 and -7[J]. Biochemistry,2001,40(4):1117-1123.
[8] Hay BA. Understanding IAP function and regulation: a view from Drosophila[J]. Cell Death Differ,2000,7(11):1045-1056.
[9] Duncan WC,Vanden Driesche S,Fraser HM. Inhibition of vascular endot helial growth factor in the primate ovary up-regulates hypoxia-inducible factor-1alpha in the follicle and corpus luteum[J]. Endocrinology,2008,149(7):3313-3320.
[10] 朱红霞,刘爽,周翠琦,等.抗凋亡基因survivin促进细胞转化的作用机制[J].中华医学杂志,2002,82(5):338-340.
[11] Mehdi Mesri,Manuel Morales-Ruiz,Elizabeth J,et al. Suppression of vascular endothelial growth factor-mediated endothelial cell protection by survivin targeting[J].Pathology,2001,158(5):1757-1765.
[12] Shin S, Lee YJ, Lee YE, et al. Protective effect of vascular endothelial growth factor(VEGF)in frozen-thawed granulosa cells is mediated by inhibition of apoptosis[J]. Eur J Obstet Gynecol Reprod Biol,2006,125(2):233-238.

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 ZHAO Yan-jiao,LIU Xin-lan,MA Zhi-lan.Expression and Clinical Significance of VEGF-C, VEGFR-3 and LYVE-1 in Gastric Cancer[J].Ningxia Medical University,2013,(07):378.

备注/Memo

备注/Memo:
收稿日期:2012-03-21 基金项目:国家自然科学基金(81160085); 宁夏自然科学基金重点项目(NZ1084); 宁夏科技攻关项目(2007GG071) 作者简介:张晓光(1986-),男,在读硕士研究生。 通信作者:王燕蓉(1957-),教授。E-mail:4083304@163.com
更新日期/Last Update: 2012-07-20