|本期目录/Table of Contents|

[1]刘丽华,王元,王娅娜,等.枸杞多糖对细粒棘球蚴小鼠T淋巴细胞亚群的影响[J].宁夏医科大学学报,2012,(05):432-434,547.
 LIU Li-hua,WANG Yuan,WANG Ya-na,et al.The Effects of LBP on Lymphocyte Subsets in Echinococcus Granulosus Mice[J].Ningxia Medical University,2012,(05):432-434,547.
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枸杞多糖对细粒棘球蚴小鼠T淋巴细胞亚群的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年05期
页码:
432-434,547
栏目:
回医药学
出版日期:
2012-05-30

文章信息/Info

Title:
The Effects of LBP on Lymphocyte Subsets in Echinococcus Granulosus Mice
作者:
刘丽华1王元2王娅娜2赵巍2赵嘉庆12
1.宁夏医科大学基础医学院病原生物学与免疫学系,银川750004;2.宁夏医科大学医学科学技术研究中心,银川750004
Author(s):
LIU Li-hua1WANG Yuan2WANG Ya-na2ZHAO Wei2ZHAO Jia-qing12
1.Department of Microbiology and Immunology, Ningxia Medical University,Yinchuan 750004; 2. Medical Scientific Center of Ningxia Medical University,Yinchuan 750004
关键词:
枸杞多糖细粒棘球蚴流式细胞仪T淋巴细胞
Keywords:
LBPechinococcus granulosusflow cytometrylymphocytes
分类号:
R383.3+3
DOI:
-
文献标志码:
A
摘要:
目的研究枸杞多糖(LyciumBarbarumPolysaccharides,LBPs)对细粒棘球蚴小鼠脾脏T细胞亚群的影响,初步探讨LBP对细粒棘球蚴感染后机体免疫功能变化的影响。方法枸杞多糖溶液皮下给药3次,每次间隔2周,于第3次给药后2周,用细粒棘球蚴原头蚴攻击感染BALB/c小鼠,使用流式细胞仪动态检测给药前后、感染前后不同时间点小鼠脾脏IFN-γ+CD3+T细胞、IL-4+CD3+T细胞占总T细胞的比例以及CD4+/CD8+T细胞的比值变化。结果与给药前比较,枸杞多糖给药后7周,小鼠脾脏分泌IFN-γ的T细胞比例增加,分泌IL-4的T细胞比例减少,CD4+/CD8+的比例明显增加;与感染对照组相比,枸杞多糖能使细粒棘球蚴感染小鼠脾脏分泌IFN-γ的T细胞比例增加(感染后2、15周);分泌IL-4的T细胞比例减少(感染后2、10周),CD4+/CD8+的比例先升高(2、10周),后降低(15、20周)。结论枸杞多糖有正向免疫调节作用,通过促进细粒棘球蚴感染小鼠脾脏T淋巴细胞向Th1的分化,抑制Th2的分化,发挥抗细粒棘球蚴的作用。
Abstract:
Objective To explore effects of LBP (Lycium barbarum polysaccharide) on lymphocyte subsets in echinococcus granulosus mice and to disscuss the immune role of LBP in echinococcus infected body.Methods LBP solution was injected subcutaneous to BALB/c mice 3 times at 2w interval. Mice were infected with Echinococcus granulosus larva at 8th w. IFN-γ+,IL-4+and CD4+/CD8+ in mice were detected with Flow cytometry before and after the LBP administration and Echinococcus granulosus infection. Results compared with no LBP group,LBP made IFN-γ increase,IL-4 reduce,CD4+/CD8+ increase significantly in BALB/c mice's lymphocyte's T cell. Compared with Echinococcus granulosus mice,LBP made IFN-γ increase(2,15w),IL-4 reduce(2,10w),CD4+/CD8+ increase(2,10w),then reduce(15,20w)in Echinococcus granulosus mouse models' lymphocyte's T cell.Conclusion it shows that LBP has the positive role of immune regulation by promoting T lymphocytes in Echinococcus granulosus infected mice to Th1 differentiation,inhibiting Th2 differentiation in BALB/c mouse.

参考文献/References:

[1]Haiyang Gong,Ping Shen,Li Jin,et al.Therapeutic Effects of Lycium barbarum Polysaccharide (LBP)on Irradiation or Chemotherapy-Induced Myelosuppressive Mice[J].Cancer Biotherapy & Radiopharmaceuticals,2005,20(2):155-162.
[2]Lu Gan,Sheng Hua Zhang,Xiang Liang Yang,et al.Immunomodulation and antitumor activity by a polysaccharideprotein complex from Lycium barbarum.[J].International Immunopharmacology,2004,4(4):563-569.[JP]
[3]李光富,张兆松.血吸虫感染致Th1/ Th2效应选择及其抗感染作用的分子机制[J].国外医学:寄生虫病分册,2003,30(4):157-161.
[4]夏超明,龚唯,骆伟,等.日本血吸虫感染小鼠肝肉芽肿与Th1/Th2细胞因子水平的动态变化[J].复旦学报:医学科学版,2001,28(1):39-46.

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备注/Memo

备注/Memo:
收稿日期:2011-12-20 基金项目:国家自然科学基金(30960340);宁夏自然科学基金(NZ11211) 作者简介:刘丽华(1981-),女,在读硕士研究生。 通信作者:[HTSS]赵嘉庆(1973-),男,副教授,硕士研究生导师。E-mail:nxmczhaojq@163.com
更新日期/Last Update: 2012-05-20