|本期目录/Table of Contents|

[1]赵春梅,刘 强,王振海.HSE模型小鼠的水迷宫实验观察[J].宁夏医科大学学报,2012,(02):115-117.
 ZHAO Chun-mei,LIU Qiang,WANG Zhen-hai.Behavioural Comparision of HSE Mice in Morris Water Maze Experiment[J].Ningxia Medical University,2012,(02):115-117.
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HSE模型小鼠的水迷宫实验观察(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年02期
页码:
115-117
栏目:
论著
出版日期:
2012-02-28

文章信息/Info

Title:
Behavioural Comparision of HSE Mice in Morris Water Maze Experiment
作者:
赵春梅 刘 强 王振海
宁夏医科大学,宁夏颅脑疾病重点实验室,银川 750004
Author(s):
ZHAO Chun-mei LIU Qiang WANG Zhen-hai
Ningxia Medical University, Yinchuan 750004
关键词:
单纯疱疹病毒-1 认知功能 Morris水迷宫 小鼠
Keywords:
herpes simplex encephalitis cognition morris water maze tests mice
分类号:
R74
DOI:
-
文献标志码:
A
摘要:
目的 对HSE模型小鼠进行经典的Morris水迷宫实验,以观察评估HSV-1感染后小鼠的学习记忆功能。方法 雄性C57BL/6小鼠100只,随机法分为模型组、假手术组、正常组。采用颅内注射法建立HSE动物模型,接种后每日观察各组小鼠的一般状况,并于感染后20天进行经典的Morris水迷宫实验评价认知功能。水迷宫实验共训练5d,记录每天的潜伏期与游泳轨迹,第6天去除平台,记录小鼠在目标象限的停留时间。结果 接种后3天开始,模型组小鼠出现进食减少、毛发蓬松、倦怠少动等症状; 水迷宫实验中模型组小鼠逃避潜伏期明显延长,目标象限停留时间及经过平台次数较其它两组明显减少(P<0.05)。结论 HSV-1感染后小鼠认知功能明显减退。
Abstract:
Objective To observe the cognition of mice with herpes simplex encephalitis. Methods 100 male C57BL/6 mice were randomly divided into three groups: the normal group,the sham-operated group and the model group. Herpes simplex encephalitis mice model was established by Intracerebral inoculation. The morris water maze tests were done respectively at the 20th day after inoculation. All animals were tested for 5 consecutive days, in which escaping latency and swimming track were recorded automatically. On the 6th day the platform was removed and the time in target quadrant were analyzed. Results Compared with the sham-operated group and normal group,the model group displayed much longer latencies and spent less time in target quadrant. Conclusion Mice with herpes simplex encephalitis displayed cognitive impairment obviously.

参考文献/References:

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[2] 李爱萍, 赵慧, 李韶, 等. 不同鼠种在Morris水迷宫学习记忆行为中的种属差异[J]. 中国行为医学科学, 2005,14(1):29-31.
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备注/Memo

备注/Memo:
收稿日期:2011-08-05 基金项目:2011年国家自然科学基金项目(81160151); 教育部春晖计划资助项目(Z-2008-1-75002); 2009年宁夏医科大学面上项目(XM200954) 作者简介:赵春梅(1976-),女,在读硕士研究生。E-mail:zcmlxf@163.com 通信作者:王振海(1968-),男,教授,博士。E-mail: wangzhenhai1968@163.com
更新日期/Last Update: 2012-02-20