|本期目录/Table of Contents|

[1]惠 晶,赵春梅,刘 强,等.S100BmRNA在单纯疱疹病毒性脑炎小鼠脑组织中的表达[J].宁夏医科大学学报,2012,(02):103-105,109.
 HUI Jing,ZHAO Chun-mei,LIU Qiang,et al.Expression of S100BmRNA in Brain Tissue of Mice with Herpes Simplex Virus Encephalitis[J].Ningxia Medical University,2012,(02):103-105,109.
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S100BmRNA在单纯疱疹病毒性脑炎小鼠脑组织中的表达(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年02期
页码:
103-105,109
栏目:
论著
出版日期:
2012-02-28

文章信息/Info

Title:
Expression of S100BmRNA in Brain Tissue of Mice with Herpes Simplex Virus Encephalitis
作者:
惠 晶13 赵春梅13 刘 强13 王振海23
1.宁夏医科大学,银川 750004; 2.宁夏医科大学总医院,银川 750004; 3.宁夏颅脑疾病重点实验室,银川 750004
Author(s):
HUI Jing13 ZHAO Chun-mei13 LIU Qiang13 WANG Zhen-hai23
1.Ningxia Medical University, Yinchuan 750004; 2.The General Hospital of Ningxia Medical University, Yinchuan 750004; 3.Key Laboratory of Brain Disease of Ningxia,Yinchuan 750004
关键词:
单纯疱疹病毒脑炎 小鼠 S100B
Keywords:
HSE Mice S100B
分类号:
R512.3
DOI:
-
文献标志码:
A
摘要:
目的 检测单纯疱疹病毒性脑炎(HSE)小鼠脑组织中S100BmRNA的含量,初步探讨S100BmRNA对HSE小鼠脑损害的评估价值。方法 使用已制造成功的HSE小鼠模型48只,分为HSE组、正常组、假手术组,分别提取小鼠脑组织总RNA,反转录为cDNA,采用RT-qPCR检测S100BmRNA含量,并对S100BmRNA进行测序。结果 HSE组S100B含量显著高于假手术及正常组(P<0.01); S100B基因测序结果在BLAST中进行比对,显示同源性100%证实合成S100B基因片段的正确。结论 S100B在HSE、正常、假手术组均有表达,但其表达的程度并不一致,提示S100B可能为HSE小鼠脑损害的标志物,其含量的高低与损害程度有关。
Abstract:
Objective To detect levels of S100B in brain tissue of mice with herpes simplex virus encephalitis(HSE)and to explore the value of S100BmRNA on preliminary study on assessment of brain damage in HSE mice. Methods 48 HSE mice were divided into HSE, normal, sham-operated group. Total RNA were extracted from mouse brain and reverse transcribed into cDNA. S100B mRNA levels were detected using RT-qPCR. Results S100B in HSE group were significantly higher than those in the sham and normal group(P<0.01). S100B gene sequencing showed 100% homology S100B synthesis. Conclusion S-100B were successfully expressed in the HSE, the normal, sham group. HSE may prompt S100B marker of brain damage in mice and the levels are correlated with the degree of damage.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-05-08 基金项目:国家自然科学基金地区科学基金项目(81160151); 教育部春晖计划资助项目(Z-2008-1-75002) 作者简介:惠晶,女,在读硕士研究生。E-mail:343738751qq@.com 通信作者:王振海(1968-),男,教授,博士。E-mail: wangzhenhai1968@163.com
更新日期/Last Update: 2012-02-20