|本期目录/Table of Contents|

[1]赵 凯,钱月慧,程晓东.TNF-α、NF-κB在动脉粥样硬化闭塞症兔动脉中的表达变化[J].宁夏医科大学学报,2012,(01):14-16.
 ZHAO Kai,QIAN Yue-hui,CHENG Xiao-dong.The Level Changes of TNF-α 、NF-κB on Rabbit Artery with Atherosclerotic Occlusive Disease[J].Ningxia Medical University,2012,(01):14-16.
点击复制

TNF-α、NF-κB在动脉粥样硬化闭塞症兔动脉中的表达变化(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年01期
页码:
14-16
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
The Level Changes of TNF-α 、NF-κB on Rabbit Artery with Atherosclerotic Occlusive Disease
作者:
赵 凯1钱月慧2 程晓东3
1.宁夏医科大学总医院中医科,银川 750004; 2.宁夏医科大学中医学院,银川 750004; 3.美国Wisconsin血液研究所
Author(s):
ZHAO Kai QIAN Yue-hui CHENG Xiao-dong
Traditional Chinese Medicine Department,General Hospital,Ningxia Medical University,Yinchuan 750004
关键词:
动脉粥样硬化 TNF-α NF-κB
Keywords:
atherosclerotic occlusive disease animal model atherogenic index aortic intima thickness inflammatory cytokines
分类号:
R543.1
DOI:
-
文献标志码:
A
摘要:
目的 探讨动脉粥样硬化闭塞症(ASO)兔模型TNF-α、NF-κB mRNA和蛋白表达的变化,部分阐明ASO炎症反应机制。方法 建立ASO动物模型,以免疫组化法观察主动脉内皮组织TNF-α、NF-κB蛋白表达,RT-PCR方法观察TNF-αmRNA、NF-κBmRNA表达情况。通过免疫组化指数以及目的基因的相对转录量为参数进行统计分析。结果 ASO模型兔主动脉内膜明显增厚,斑块面积明显增加。模型组兔主动脉内皮组织TNF-α、NF-κB蛋白表达和TNF-αmRNA、NF-κBmRNA的表达均较对照组升高(P<0.01)。结论 ASO发生发展与炎症反应有关。
Abstract:
Objective To observe the level changes of TNF-α 、NF-κB and TNF-αmRNA、NF-κBmRNA in rabbit artery with the atherosclerotic occlusive disease(ASO). Methods ASO model was established in rabbits. Changes of TNF-α 、NF-κB were detected by the Immunohistochemistry and the changes of TNF-αmRNA、NF-κBmRNA were monitored by the reverse transcrip tase polymerase chain reaction assay. Results Compared the model group with the control group, the expression level of TNF-αmRNA、NF-κBmRNA showed significant increase(P<0.01). Conclusion The level of TNF-α、NF-κB and TNF-αmRNA、NF-κBmRNA were high expression on the established process of the atherosclerotic occlusive disease(ASO. The mechanism of inflammation is closely related to the atherosclerotic occlusive disease.

参考文献/References:

[1] Ross R. The pathogenesis of atherosclerosis:a perspective for the 1990s [J].Nature,1993,363:801-809.
[2] 张勇,娄桂予,钱民章.建立兔高脂血症模型及动脉粥样硬化斑块的办法[J].遵义医学院学报,2001,24(4):304.
[3] 吴明远,李令根,赵钢,等.家兔股动脉粥样硬化闭塞症模型的制作[J].中国中西医结合外科杂志,2004,10(1):29-31.
[4] 顾晴,陈纪林,阮英茚,等.氯吡格雷对实验性动脉粥样硬化形成的影响[J].中国循环杂志,2004,19(2):145-148.
[5] 倪灿荣.免疫组织化学实验技术及应用[M]. 北京:化学工业出版社,2006.
[6] 姜泊,张亚历,周殿元.分子生物学常用实验方法[M].北京:人民军医出版社.1996:57.
[7] 黄修献,李利华,吴新华. 血管内皮细胞与动脉粥样硬化[J]. 医学综述,2010,16,(18)2724-2726.
[8] 金惠铭,刘清行,张国平,等.TNF-α对血管内皮细胞的损伤作用及其分子机制[J].中国病理生理杂志,1999,15(8):765.
[9] Blake GJ, Ridker PM. Novei clinical markers of vascular wall inflammation[J].Circ Res,2001,89:763-771.
[10] 樊冬梅,薛莉. 核转录因子kappaB在急性心肌梗死的作用研究[J].宁夏医学杂志,2010,32(1):46.

相似文献/References:

[1]赵兴,姚成立.尿微量白蛋白与颈动脉及冠状动脉粥样硬化的相关性研究[J].宁夏医科大学学报,2009,(03):317.
 ZHAO Xing,YAO Cheng-li.[J].Ningxia Medical University,2009,(01):317.
[2]梁锦屏,王琳琳,黄 菱,等.内毒素血症小鼠肺组织损伤及血清炎症因子变化的研究[J].宁夏医科大学学报,2010,(08):852.
 LIANG Jin-ping,WANG Lin-lin,HUANG Lin,et al.Changes of Lung Injury Indices and Serum Inflammatory Factors in Mice with Endotoxemia Induced by Lipopolysaccharide[J].Ningxia Medical University,2010,(01):852.
[3]李继东,姚成立,宁海峰.还原型谷胱甘肽干预高同型半胱氨酸血症 致兔动脉粥样硬化的实验研究[J].宁夏医科大学学报,2010,(07):773.
 LI Ji-dong,YAO Cheng-li,NING Hai-feng.Glutathione Intervention Hyperhomocysteimia Induced Atherosclerosis in Rabbits[J].Ningxia Medical University,2010,(01):773.
[4]周 丽,杨明武,李继东,等.高频超声检测高同型半胱氨酸血症兔模型 动脉粥样硬化的实验研究[J].宁夏医科大学学报,2010,(05):593.
 ZHOU Li,YANG Ming-wu,LI Ji-dong,et al.Experimental Study of Atherosclerosis in Rabbit Model with Hyperhomocysteinemia by High Resolution Ultrasound[J].Ningxia Medical University,2010,(01):593.
[5]王 虎,吴继红,邓 丽,等.乌司他丁对体外循环下瓣膜置换术患者 TNF-α、MDA、PMN的影响[J].宁夏医科大学学报,2010,(04):494.
 WANG Hu,WU JI-hong,DENG Li,et al.The Influence of Ulinastatin on TNF-α, MDA, PMN during Cardio-pulmonary Bypass in Patients undergoing Heart Valve Replacement[J].Ningxia Medical University,2010,(01):494.
[6]纳 莉,徐支芳,巩慧慧,等.同型半胱氨酸致动脉粥样硬化的机制研究[J].宁夏医科大学学报,2012,(09):887.
 NA Li,XU Zhi-fang,GONG Hui-hui,et al.Induced Hyperhommocysteinemia in the Study of the Mechanisms of Otherosclerosis[J].Ningxia Medical University,2012,(01):887.
[7]单晓宇,刘敬霞,楚国庆,等.回药扎里奴思方对脑梗死患者颈动脉硬化的影响[J].宁夏医科大学学报,2012,(08):753.
 SHAN Xiao-yu,LIU Jing-xia,CHU Guo-qing,et al.Effect of Hui Medicine Zhalinusi Recipe on Carotid Atherosclerosis Changes in Patients with Cerebral Infarction in Patients[J].Ningxia Medical University,2012,(01):753.
[8]李 录,贾绍斌.DNA甲基化与动脉粥样硬化的研究进展[J].宁夏医科大学学报,2013,(05):598.
[9]赵兴,郭健伟,刘欢,等.原发性高血压患者红细胞和血小板参数的变化与颈动脉粥样硬化的关系[J].宁夏医科大学学报,2013,(02):206.
[10]王文娟,孟尽海,姚 杰,等.瑞芬太尼预先给药对失血性休克兔肝损伤时脂质过氧化的影响[J].宁夏医科大学学报,2013,(02):130.
 WANG Wen-juan,MENG Jin-hai,YAO jie,et al.Effects of Remifentanil on Lipid Peroxidation during Hemorrhagic Shock-induced Liver Injury in Rabbits[J].Ningxia Medical University,2013,(01):130.

备注/Memo

备注/Memo:
收稿日期:2011-10-24 基金项目:国家教育部春晖计划项目 作者简介:赵凯(1969-),男(满族),北京人,主任医师,博士,硕士研究生导师,从事中西医结合风湿性疾病、周围血管病和内分泌代谢性疾病的治疗和研究工作。
更新日期/Last Update: 2012-01-20