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[1]于海娇,徐支芳,马琳娜,等.同型半胱氨酸对血管平滑肌细胞增殖 和细胞周期的影响及叶酸的拮抗作用[J].宁夏医科大学学报,2012,(01):7-9.
 YU Hai-jiao,XU Zhi-fang,MA Lin-na,et al.The Effect of Homocysteine on the Proliferation of Vascular Smooth Muscle Cells in Vitro and the Antagonizing Effect of Folic Acid[J].Ningxia Medical University,2012,(01):7-9.
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同型半胱氨酸对血管平滑肌细胞增殖 和细胞周期的影响及叶酸的拮抗作用(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年01期
页码:
7-9
栏目:
论著
出版日期:
2012-01-30

文章信息/Info

Title:
The Effect of Homocysteine on the Proliferation of Vascular Smooth Muscle Cells in Vitro and the Antagonizing Effect of Folic Acid
作者:
于海娇1 徐支芳2 马琳娜1 吴梦莹1 姜怡邓1
1.宁夏医科大学检验学院,银川 750004; 2.宁夏医科大学基础医学院,银川 750004
Author(s):
YU Hai-jiao1 XU Zhi-fang2 MA Lin-na1 WU Meng-Ying1 JIANG Yi-deng1
1.Dept.of Inspection,Ningxia Med. Univ.,Yinchuan 750004; 2.Dept.of Basic Medicine College,Ningxia Med.Univ.,Yinchuan 750004
关键词:
同型半胱氨酸 血管平滑肌细胞 细胞增殖 细胞周期
Keywords:
homocysteine vascular smooth muscle cell proliferation cell cycle
分类号:
R363
DOI:
-
文献标志码:
A
摘要:
目的 探讨不同浓度同型半胱氨酸(Hcy)对血管平滑肌细胞(VSMCs)增殖和细胞周期分布的影响及叶酸对Hcy的拮抗作用。方法 正常人脐动脉VSMCs体外培养,随机分为6组:空白对照组、不同浓度Hcy干预组(50、100、200、500μmol·L-1)和叶酸治疗组(Hcy100μmol·L-1+叶酸30μmol·L-1)。应用MTT法及流式细胞仪检测各组VSMCs增殖率及细胞周期。结果 随着Hcy浓度的增加VSMCs的增殖率增加; Hcy作用后VSMCs G0/G1期细胞数降低; S期和G2/M期细胞数增加,处于DNA合成期的细胞数明显增加,而叶酸治疗组与空白对照组比较差异无统计学意义(P>0.05),叶酸治疗组与Hcy 100μmol·L-1组比较差异有统计学意义(P<0.05)。结论 Hcy可通过对细胞周期的影响促进VSMCs的增殖,并在一定时间和浓度范围内呈明显的剂量依赖关系; 叶酸对Hcy有拮抗作用。
Abstract:
Objective To explore the effect of homocysteine(Hcy)on the proliferation of vascular smooth muscle cells(VSMCs)and the antagonizing effect of folic acid. Methods Cultured VSMCs were randomly divided into black control group, different concentrations of Hcy group(50,100,200,500μM)and folic acid treated group(Hcy100μM+folic acid 30μM). The effect of folic acid treated group and different concentrations of Hcy group on the proliferation rate and cell cycle of VSMCs were detected by MTT and cell cycle flow cytometer. Results With Hcy concentration increasing, the multiplication rate of the VSMCs increased; and the VSMCs in the G0/G1 phase decreased and in the S and G2/M phase also increaced. It meant that the VSMCs number in the synthesis period of DNA increased significantly. There were no significant changes between folic acid treated group and black control group. VSMCs between folic acid treated groupand Hcy 100μmol·L-1 group showed significant difference(P<0.05 ). Conclusion Hcy can promote the proliferation of VSMCs in a manner of dose dependent and time dependent; folic acid has the antagonistic effects on Hcy.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2011-07-20 基金项目:国家自然科学基金项目(30960124); 宁夏医科大学校级课题(宁医校发[2011]42号) 作者简介:于海娇(1984-),女,黑龙江人,在读硕士研究生。 通信作者:姜怡邓(1974-),男,湖南人,副教授,博士。E-mail:jiangyideng123@163.com
更新日期/Last Update: 2012-01-20