|本期目录/Table of Contents|

[1]杨彩虹,李小涛,张雪玉,等.血管内皮生长因子C在宫颈癌中表达的临床研究[J].宁夏医科大学学报,2011,(11):1048-1050,1057.
 YANG Cai-hong,LI Xiao-tao,ZHANG Xue-yu,et al.Clinical Research on the Expression of VEGF-C in Cervical Carcinoma[J].Ningxia Medical University,2011,(11):1048-1050,1057.
点击复制

血管内皮生长因子C在宫颈癌中表达的临床研究(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2011年11期
页码:
1048-1050,1057
栏目:
论著
出版日期:
2012-12-30

文章信息/Info

Title:
Clinical Research on the Expression of VEGF-C in Cervical Carcinoma
作者:
杨彩虹1 李小涛2 张雪玉1 张咏梅3
1.宁夏医科大学总医院妇科,银川 750004; 2.宁夏回族自治区体检康复保健中心,银川 750004; 3.宁夏医科大学第二附属医院妇科,银川 750004
Author(s):
YANG Cai-hong1 LI Xiao-tao2 ZHANG Xue-yu1 ZHANG Yong-mei3
1.The General Hospital of Ningxia Medical University, Yinchuan 750004; 2.Physical Examination Recovery Health center of Ningxia, Yinchuan 750004; 3.Department of Gynecology, the Second Affiliated Hospital of Ningxia Medical University, Yinchuan 750001
关键词:
宫颈癌 VEGF-C 荧光定量RT -PCR ELISA
Keywords:
cervical carcinoma VEGF-C fluorescent quantitation RT-PCR ELISA
分类号:
R711.74
DOI:
-
文献标志码:
A
摘要:
目的 检测宫颈癌患者血管内皮生长因子C(Vascular endothelial growth factor-C,VEGF-C)表达水平,探讨宫颈癌患者VEGF-C表达水平的临床意义。方法 采用荧光定量RT-PCR(Real Time Fluorescence Quantitative PCR,FQ RT-PCR)技术定量检测60例宫颈浸润癌和20例正常宫颈组织标本中VEGF-C mRNA表达水平; 采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定同一患者血清VEGF-C浓度。结果 宫颈癌患者组织标本中VEGF-C mRNA水平及血清中VEGF-C浓度显著高于正常宫颈者(P<0.01),并且随着临床期别的升高依次升高(P=0.000),与肿瘤的浸润深度和有无淋巴结转移有关(P<0.05),而与肿瘤的组织类型、分化程度无关(P>0.05)。宫颈癌患者组织中VEGF-C mRNA表达量与血清中VEGF-C浓度呈正相关(P<0.01,r=0.517)。结论 VEGF-C可以成为宫颈癌术前预测盆腔淋巴结转移及预后评估的一个因子。
Abstract:
Objective To investigate the clinical significance of vascular endothelial growth factor-C(VEGF-C)in cervical carcinoma and normal cervical. Methods(1)The mRNA levels of VEGF-C was detected by Real Time Fluorescence Quantitative PCR(FQ RT-PCR)method,and the serum levels was determined by enzyme linked immunosorbent assay(ELISA)in 60 cervical carcer. Results Levels of mRNA expression and concentration of serum of VEGF-C in cervical carcinoma were significantly higher than those in normal cervix. Levels of VEGF-C showed increasing with the development of clinic stage cervical carcinoma(P<0.05),and which were correlated with grade depth of stromas invasion and lymph node involvement(P<0.05). VEGF-C mRNA expression quantity in cervical cancer tissue were positively correlated with serum VEGF-C concentrations. Conclusions VEGF-C could be a factor to predict pelvic lymph node preoperative metastasis and prognosis in patients with cervical cancer.

参考文献/References:

[1] Takahashi H, Shibuya M.The vascular endothelial growth factor(VEGF)/VEGF receptor system and its role under physiological and pathological conditions[J].Clin Sci(Lond), 2005, 109(3):227-241.
[2] Dadras SS,Lange-asschenfeldt B,Velasco P, et al. Tumor lymphangiogenesis predicts melanoma metastasis to sentinel lymph nodes[J].Mod Pathol, 2005, 18(9):1232-1242.
[3] Mitsuhashi A, Suzuka K,Yamazawa K,et al. Serum vascular endothelial growth factor(VEGF)and VEGF-C levels as tumormarkers in patientswith cervical carcinoma[ J]. Cancer, 2005, 103(4):724-730.
[4] 吴玉梅.早期宫颈癌淋巴管新生与淋巴结转移的相关性研究[D].第三军医大学,2009.
[5] Hashimoto I, Kodama J, Seki N, et al. Vascular endothelial growth factor-C expression and its relationship to pelvic; lymph node status in invisive cervical cancer[J]. Br J Cancer, 2001,85(1):93-97. (责任编辑:路锦绣) Clinical Research on the Expression of VEGF-C in Cervical Carcinoma YANG Cai-hong1, LI Xiao-tao2, ZHANG Xue-yu1, ZHANG Yong-mei3 (1.The General Hospital of Ningxia Medical University, Yinchuan 750004; 2.Physical Examination Recovery Health center of Ningxia, Yinchuan 750004; 3.Department of Gynecology, the Second Affiliated Hospital of Ningxia Medical University, Yinchuan 750001)Abstract: Objective To investigate the clinical significance of vascular endothelial growth factor-C(VEGF-C)in cervical carcinoma and normal cervical. Methods(1)The mRNA levels of VEGF-C was detected by Real Time Fluorescence Quantitative PCR(FQ RT-PCR)method,and the serum levels was determined by enzyme linked immunosorbent assay(ELISA)in 60 cervical carcer. Results Levels of mRNA expression and concentration of serum of VEGF-C in cervical carcinoma were significantly higher

相似文献/References:

[1]马建萍,赵 仁,何剑莉,等.中药制剂直肠外用防治宫颈癌放疗所致放射性直肠炎的临床研究[J].宁夏医科大学学报,2011,(12):1147.
 MA Jian-ping,ZHAO Ren,HE Jian-li,et al.The Prevention and Treatment of the Radiation-induced Proctitis with Herbal Medicine Administered to Rectum in Patientswith Cervical Carcinoma[J].Ningxia Medical University,2011,(11):1147.
[2]徐秀琴,王玉林,谭红霞,等.平罗县农村妇女宫颈癌筛查结果分析[J].宁夏医科大学学报,2011,(10):998.
[3]常 琦,张治宁.310例宫颈癌根治术后并发盆腔淋巴囊肿的临床分析[J].宁夏医科大学学报,2011,(01):35.
 CHANG Qi,ZHANG Zhi-ning.A Clinical Analysis of Lymphocyst after Radical Hysterectomy in 310 Cases[J].Ningxia Medical University,2011,(11):35.
[4]王智宇,张雪玉.764例宫颈癌临床资料分析及治疗模式变化的探讨[J].宁夏医科大学学报,2009,(01):47.
 WANG Zhi-yu,ZHANG Xue-yu.[J].Ningxia Medical University,2009,(11):47.
[5]王智宇,张雪玉,包生武,等.肝细胞生长因子及其受体与ⅡA期宫颈癌临床病理关系分析[J].宁夏医科大学学报,2010,(05):572.
 WANG Zhi-yu,ZHANG Xue-yu,BAO Sheng-wu,et al.Relationship between Expression of HGF/c-Met and Clinicopathological Properties in Uterine Cervix Carcinomas[J].Ningxia Medical University,2010,(11):572.
[6]郭玉娥,杨尧华.MMP-14在宫颈鳞癌中的表达及临床意义[J].宁夏医科大学学报,2010,(02):223.
 GUO Yu-e,YANG Yao-hua.Expression and Clinical Significance of MMP-14 Protein inSquamous Cell Carcinoma of Cervix[J].Ningxia Medical University,2010,(11):223.
[7]马永静,熊 英,刘 瑞.CK19mRNA检测在判断IA2-IB2期宫颈癌 前哨淋巴结微转移中的作用[J].宁夏医科大学学报,2012,(11):1122.
 Application of CK9mRNA in Evaluating Sentinel Lymph Node Micrometastasis in Patients with Stage IA-IB Cervical Cancer.Application of CK19mRNA in Evaluating Sentinel Lymph Node Micrometastasis in Patients with Stage IA2-IB2 Cervical Cancer[J].Ningxia Medical University,2012,(11):1122.
[8]张宇卉,折 虹,王艳阳,等.LIG4mRNA与XRCC4mRNA在宫颈癌中的表达及临床意义[J].宁夏医科大学学报,2012,(03):215.
 ZHANG Yu-hui,ZHE Hong,WANG Yan-yang,et al.Expression and Clinical Significance of LIG4 mRNA and XRCC4 mRNA in Cervical Carcinoma[J].Ningxia Medical University,2012,(11):215.
[9]陈永红.HPV16与宫颈病变的相关性分析[J].宁夏医科大学学报,2013,(06):718.
[10]张宇卉,折 虹,王艳阳,等.宫颈癌组织LIG4、XRCC4、XRCC5、XRCC6、XRCC7的 mRNA表达及与放疗敏感性的关系[J].宁夏医科大学学报,2013,(04):398.
 ZHANG Yu-hui,ZHE Hong,WANG Yan-yang,et al.The Relationship between Radiosensitivity and DNA Double Strand Breaks Repair Genes in Non-homologous End Joining Pathway in Human Cervical Carcinoma[J].Ningxia Medical University,2013,(11):398.

备注/Memo

备注/Memo:
收稿日期:2011-07-18 基金项目:宁夏自然科学基金资助(编号NZ10144) 作者简介:杨彩虹(1971-),硕士。E-mail: yangcaihong197107@163.com 通信作者:张雪玉,教授,硕士研究生导师。E-mail: zhxueyu@163.com
更新日期/Last Update: 2011-11-20