|本期目录/Table of Contents|

[1]王占伟,乔卫军,赵 巍,等.重组穿梭质粒pAdTrack-cmv-rHSG载体的构建及鉴定[J].宁夏医科大学学报,2011,(02):119-121,129.
 WANG Zhan-wei,QIAO Wei-jun,ZHAO Wei,et al.Construction and Identification of Recombinant Plasmid pAdTrack-cmv-rHSG Encoding the Rat rHSG Gene[J].Ningxia Medical University,2011,(02):119-121,129.
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重组穿梭质粒pAdTrack-cmv-rHSG载体的构建及鉴定(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2011年02期
页码:
119-121,129
栏目:
论著
出版日期:
2012-12-30

文章信息/Info

Title:
Construction and Identification of Recombinant Plasmid pAdTrack-cmv-rHSG Encoding the Rat rHSG Gene
作者:
王占伟1 乔卫军2 赵 巍3 沈 冰1
1.宁夏医科大学附属医院神经外科,银川 750004; 2.陕西咸阳市中心医院神经外科,咸阳 712000; 3.宁夏医科大学医学科学技术研究中心,银川 750004
Author(s):
WANG Zhan-wei1QIAO Wei-jun2 ZHAO Wei3 SHEN Bing1
1.Department of Neurosurgery, the Affiliated Hospital of Ningxia Medical University. Yingchuan 750004; 2.Department of Neurosurgery, Xianyang Central Hospital, Xianyang 712000; 3.Science and Technology Department of Ningxia Medical University, Yingchuan
关键词:
腺病毒穿梭质粒 增殖抑制基因 恶性胶质瘤
Keywords:
adenovirus shuttle plasmid hyperplasia suppressor gene spongiocytoma
分类号:
R739.41
DOI:
-
文献标志码:
A
摘要:
目的 构建大鼠增殖抑制基因(rat hyperplasia suppressor gene, rHSG)腺病毒穿梭质粒,为进一步研究体内诱导rHSG在恶性胶质瘤的特异性表达奠定基础。方法 提取SD大鼠心肌总RNA,利用RT-PCR方法扩增大鼠增殖抑制基因(rHSG)的全开放读码框区片段,将其亚克隆入T载体,双酶切后与经同样处理的腺病毒穿梭质粒pAdTrack-cmv相连,用酶切和测序进行鉴定。结果 扩增rHSG克隆出rHSG基因并成功重组于入腺病毒基因并成功的穿梭质粒。结论 构建成功重组大鼠增殖抑制基因腺病毒穿梭质粒载体pAdTrack-cmv-rHSG。
Abstract:
Objective To construct a adenovirus shuttle plasmid encoding rat hyperplasia suppressor gene(rHSG),and to provide the basis for further researching on rHSG specific expression in the spongioblastoma tissue. Methods The complementary RNA of SD rats Cardiac muscle was extracted. The whole Open reading frame fragment of the rHSG gene was amplified by RT- PCR and subcloned in the pGM-T plasmid by double enzymat cutting.It was identified by enzymatic cutting and PCR products sequencing. Results RHSG gene was amplified by RT- PCR and cloned in the adenovirus shuttle plasmid. The favorite gene sequence was completely consistent with that reported In GENEBANK. Conclusion cDNA of rHSG can be successfully cloned and inserted into pAdTrack-cmv vector.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-08-27 基金项目:宁夏回族自治区教育厅基金(V200852); 银川市科技攻关项目(V200962) 作者简介:王占伟(1977-),男,河南人,主治医师,在读硕士研究生,从事神经肿瘤研究。 通信作者:沈冰,主任医师,硕士研究生导师。E-mail:shenbing315@sina.com.cn
更新日期/Last Update: 2011-02-20