|本期目录/Table of Contents|

[1]王立斌,刘 婷,马晓娜,等.人胎盘间充质干细胞的分离与鉴定[J].宁夏医科大学学报,2010,(08):864-866.
 WANG Li-bin,LIU Ting,MA Xiao-na,et al.Isolation and Characterization of Mesenchymal Stem Cells from Human Placenta[J].Ningxia Medical University,2010,(08):864-866.
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人胎盘间充质干细胞的分离与鉴定(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年08期
页码:
864-866
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Isolation and Characterization of Mesenchymal Stem Cells from Human Placenta
作者:
王立斌 刘 婷 马晓娜 李玉奎 魏 军
宁夏医科大学附属医院宁夏人类干细胞研究所,银川 750004
Author(s):
WANG Li-bin LIU Ting MA Xiao-na LI Yu-kui WEI Jun
Affiliated Hospital of Ningxia Med. Univ., Ningxia Human Stem Cell Institute, Yinchuan 750004
关键词:
胎盘 间充质干细胞 流式细胞术
Keywords:
placenta mesenchymal stem cell flow cytometry
分类号:
R714.56
DOI:
-
文献标志码:
A
摘要:
目的 从生产后胎盘中提取胎盘间充质干细胞,并对其进行干细胞特性鉴定。方法 采用酶直接消化法从胎盘组织中提取间充质干细胞,并利用流式细胞术及染色体核型分析技术鉴定其干细胞特性。结果 成功获得胎盘间充质干细胞,培养48h后即可见贴壁细胞,形态为成纤维细胞样,漩涡状生长,传代过程中细胞形态和特性未见明显变化,经细胞表面分子及染色体核型分析,证明其具备干细胞特性。结论 用酶直接消化法所获得的胎盘间充质干细胞具备干细胞特性, 可以作为临床干细胞治疗的种子细胞。
Abstract:
Objective To explore the Character of Mesenchymal Stem Cells from Human Placenta. Methods The enzyme directory digestion method was used to isolate Mesenchymal Stem Cells from Human Placenta and flow cytometry and karyotyping were used to evaluated the character of stem cells. Results Flow cytometry and karyotyping experiments showed that the cells matched with the character of Mesenchymal Stem Cells. Conclusion The enzyme digestion method can effectively isolate mesenchymal stem cells which could be ideal seed-cells for clinical stem cell implantation.

参考文献/References:

[1] In't Anker PS, Scherjon SA, Kleijburg-van der Keur C, et al. Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta[J]. STEM CELLS, 2004, 22:1338-1345.
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[3] Portmann-Lanz CB, Schoeberlein A, Huber A,et al. Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration[J]. Am J Obstet Gynecol, 2006, 194:664-673.
[4] Soncini M, Vertua E, Gibelli L,et al. Isolation and characterization of mesenchymal cells from human fetal membranes[J]. J Tissue Eng Regen Med, 2007, 1:296-305.
[5] 周新伏,旷文勇,罗自勉,等.脐带血清体外培养临床用人骨髓间充质干细胞的方法研究[J].临床血液学杂志,2008,21(9):477-480.
[6] Wei JP, Zhang TS, Kawa S,et al. Human amnion-isolated cells normalize blood glucose in streptozotocin-induced diabetic mice[J]. Cell Transplant, 2003, 12:545-552.

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备注/Memo

备注/Memo:
收稿日期:2010-07-07 基金项目:国家自然科学基金资助(30960176) 作者简介:王立斌(1976-),男(回族),主管技师,博士。 通信作者:魏军,女,硕士研究生导师,教授,主要从事干细胞免疫学研究。E-mail:lydiajunwei@hotmail.com
更新日期/Last Update: 2010-08-20