|本期目录/Table of Contents|

[1]荆 忻,朱金源,马希刚.中分子羟乙基淀粉对大鼠失血性休克早期肺保护的研究[J].宁夏医科大学学报,2010,(08):856-859.
 JING Xin,ZHU Jin-yuan,MA Xi-gang.Protective Effects of Hydroxyethyl Starch on the Acute Lung Injury following Hemorrhagic Shock[J].Ningxia Medical University,2010,(08):856-859.
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中分子羟乙基淀粉对大鼠失血性休克早期肺保护的研究(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年08期
页码:
856-859
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Protective Effects of Hydroxyethyl Starch on the Acute Lung Injury following Hemorrhagic Shock
作者:
荆 忻 朱金源 马希刚
宁夏医科大学附属医院ICU, 银川 750004
Author(s):
JING Xin ZHU Jin-yuan MA Xi-gang
ICU of the Affiliated Hospital of Ningxia Med. Univ.,Yinchuan 750004
关键词:
急性肺损伤 黏附分子 中性粒细胞弹性蛋白酶 羟乙基淀粉 液体复苏 大鼠
Keywords:
acute lung injury adhesion molecule neutrophil elastase hydroxyethyl starch liquid resuscitation rats
分类号:
R749.054
DOI:
-
文献标志码:
A
摘要:
目的 探讨中分子羟乙基淀粉溶液对大鼠失血性休克早期肺保护作用及其机制。方法 采用Wiggers改良法制作失血性休克模型。48只大鼠随机分为:假手术组(sham组)、乳酸盐林格液组(LRS组)、羟乙基淀粉40氯化钠溶液组(HE40组)和羟乙基淀粉130/0.4氯化钠溶液组(HE130组),每组12只。休克后60min后分别行液体复苏治疗,检测不同时相外周血中性粒细胞(PMN)表面黏附分子CD11a、CD11b、CD18的平均荧光强度和中性粒细胞弹性蛋白酶(NE)活性,术毕观察肺湿干质量比(W/D)、肺通透系数(LPI)和肺损伤评分(LIS)的变化。结果 失血性休克后PMN表面CD11a、CD11b、CD18表达及NE活性即同时升高,失血性休克后4h肺W/D比、LPI和LIS升高,羟乙基淀粉130/0.4氯化钠溶液可明显减弱上述改变(P<0.01)。结论 中分子羟乙基淀粉130/0.4氯化钠溶液对大鼠失血性休克早期急性肺损伤有一定保护作用,可能与下调PMN表面CD11a、CD11b和CD18的表达及抑制NE的释放有关。
Abstract:
Objective To explore the protective effects of 6% hydroxyethyl starch on the acute lung injury following hemorrhagic shock. Methods 40 Wistar rats were randomly divided into four experimental groups: the control group(Sham)the lactated Ringers group(LRS),the 6% hydroxyethyl starch 40 group(HE40)and 6% hydroxyethyl starch 130 group(HE130). Hemorrhagic shock was induced according to Wiggers and left femoral artery was cannulated for BP monitoring. MAP was maintained at 45 mm Hg for 45 minues. The positive expression rates of CD11a,CD11b,CD18 and elastase activity in neutrophil from peripheral blood were quantitatively analyzed by flow cytometry and spectrocolorimetry in four groups after 0,0.5,1,2,3,4h of establishing model. W/D ratio,lung permeability index(LPI)and lung injury score(LIS)were measured. Results The expression of CD11a,CD11b,CD18 and elastase activity in neutrophil in three experimental groups were significantly higher than those in sham group after hemorrhogic shock(P<0.01, respectively). Lung W/D ratio,LPI and LIS in three experimental groups were significantly higher than those in sham group(P<0.01, respectively).The expression of CD11a,CD11b,CD18 and elastase activity in neutrophil and lung W/D ratio,LPI and LIS in HE130 group were significantly lowerer than those in LRS and HE40 groups(P<0.01, respectively). Conclusion 6% hydroxyethyl starch 130 might significantly alleviate the degree of lung inflammatory injury by inhibiting expression rates of CD11a,CD11b,CD18 and excess production of elastase in neutrophil.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2010-02-04 基金项目:宁夏医科大学重点人才科研基金(XT200713) 作者简介:荆忻(1973-),女,北京人,主治医师,硕士,从事重症医学专业。 通信作者:马希刚,主任医师,博士,从事急性肺损伤研究。 E-mail:mxgwp@sina.com
更新日期/Last Update: 2010-08-20