|本期目录/Table of Contents|

[1]雄 英,王娅娜,李宗吉,等.细粒棘球蚴中国大陆株铁蛋白生物信息学的初步分析 及其结构和功能预测[J].宁夏医科大学学报,2010,(03):327-329,333.
 XIONG Ying,WANG Ya-na,LI Zong-ji,et al.Bioinformatics Analysis for the Structure and Function of Ferritin Gene from Echinococcus Granulosus (Chinese Mainland Strain)[J].Ningxia Medical University,2010,(03):327-329,333.
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细粒棘球蚴中国大陆株铁蛋白生物信息学的初步分析 及其结构和功能预测(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年03期
页码:
327-329,333
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
Bioinformatics Analysis for the Structure and Function of Ferritin Gene from Echinococcus Granulosus (Chinese Mainland Strain)
作者:
雄 英1 王娅娜2 李宗吉2 孙俊峰1 棘怀庆1 赵 巍2
1.宁夏医科大学基础医学院医学遗传与细胞生物学教研室, 宁夏生育力保持省部共建教育部重点实验室,宁夏回族自治区遗传与生殖重点实验室,银川 750004; 2.宁夏医科大学基础医学院医学分子生物学实验室, 银川 750004
Author(s):
XIONG Ying1 WANG Ya-na2LI Zong-ji2 et al
1.Department of Medical Genetics and Cell Biology, Ningxia Medical University, Yinchuan 750004; Laboratory of Genetics and Reproduction, Yinchuan 750004; 2.Laboratory of Medical Molecular Biology,Ningxia Medical University, Yinchuan 750004
关键词:
细粒棘球蚴中国大陆株 铁蛋白 生物信息学 预测分析
Keywords:
echinococcus granulosus ferritin bioinformatics predicting analysis
分类号:
R383.3
DOI:
-
文献标志码:
A
摘要:
目的 应用生物信息学分析软件预测细粒棘球蚴中国大陆株铁蛋白(Eg. ferritin)氨基酸序列的结构与功能。方法 利用DNAman、NCBI/BLAST公共数据库对目的基因的同源性进行比较分析。应用DNAstar、Biosun 等生物分析软件对铁蛋白的二级结构、抗原表位进行预测分析,应用SWISSMODEL对蛋白的三维结构进行模拟。结果 检索Eg. ferritin氨基酸序列在其功能区的130AA内同源性为97.7%。不同生物间的同源性平均达40.79%。生物软件预测:Eg. ferritin的分子量约16.7 kD,含非极性氨基酸68个,预测其抗原表位肽段为7N-12E,36H -43V, 55S-62H, 69Q-76R, 82A-89E, 102I-107E, 117A -124S,129L-136T。结论 Eg. ferritin的结构和功能及其抗原表位的预测对选取有价值的抗原肽段提供了依据。
Abstract:
Objective To predict the structure and function of Eg.ferritin using bioinformatics method. Methods Homology of ferritin gene was analyzed Using DNAman and NCBI/BLAST database and second structure and antigen peptide of Eg.ferritin and imitate 3D structure of Eg.ferritin was predicted using DNAstar, Biosun et.al. biological softwares to. Results Deduced ferritin amino acid sequence homology was 97.7% in the 130AA and average homology of different species was 40.79% with the published ferritin gene. And it had 68 nonpolar amino acids and had 9 antigen peptide such as 7N-12E, 36H -43V, 55S-62H, 69Q-76R, 82A-89E, 102I-107E, 117A-124S, 129L-136T. Conclusions Using softwares to predict Eg.ferritin structure and antigen peptide can provide some theoretical bases for selecting some valuable antigen peptides.

参考文献/References:

[1] Wang QL, Kong B, Huang HQ, et al. Progress in Structural and Functional Study of Nanometer Protein Shell of the ferritin[J]. Progress in Chemistry, 2004, 16(4):516-519.
[2] Andrews SC.Iron storage in bacteria[J].Adv Microb Physiol,1998, 40:281.
[3] Ponka P.Recent advance in cellular iron metabolism[J].The Journal of trace elements in experimental medicine, 2003,10:201-207.
[4] Theil EC. ferritin:at the crossroads of iron and oxygen metabolism [J]. J Nutr, 2003, 5(Suppl1):49-53.
[5] 王键,师志云,赵巍,等.宁夏人细粒棘球蚴重组延伸因之-1重组蛋白特性分析及抗原表位预测[J].中国药学杂志,2008,43(9):654-657.
[6] 棘怀庆,雄英,赵巍,等.细粒棘球蚴中国大陆株谷胱甘肽S-转移酶结构与功能的生物信息学分析[J]. 宁夏医科大学学报,2009,31(2):150-153.
[7] 王娅娜,赵嘉庆,赵巍,等. 细粒棘球蚴中国大陆株铁蛋白基因分子克隆和序列分析[J].宁夏医学院学报,2006,27(4):253-255.
[8] Thompson RCA, Lymbery AJ. Echincoccus and Hydatid Disease [M]. Oxon: CAB International, 1995:50.

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备注/Memo

备注/Memo:
收稿日期:2009-12-01 资金项目:国家自然科学基金(30660176); 宁夏自然科学基金(NZ0878) 作者简介:雄英(1984-),女,在读硕士研究生。 通信作者:赵巍(1960-),男,教授,硕士研究生导师。 E-mail:zw-6915@163.com
更新日期/Last Update: 2010-03-20