|本期目录/Table of Contents|

[1]孙俊锋,棘怀庆,雄 英,等.生物信息学方法初步筛选细粒棘球蚴防治靶位蛋白[J].宁夏医科大学学报,2010,(02):172-175.
 SUN Jun-feng,JI Huai-qing,XIONG Ying,et al.High-throughput Screening Valuable Target Protein of Echinococcusgranulosus via Bioinformatics Methods[J].Ningxia Medical University,2010,(02):172-175.
点击复制

生物信息学方法初步筛选细粒棘球蚴防治靶位蛋白(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2010年02期
页码:
172-175
栏目:
论著
出版日期:
2011-12-30

文章信息/Info

Title:
High-throughput Screening Valuable Target Protein of Echinococcus granulosus via Bioinformatics Methods
作者:
孙俊锋 棘怀庆 雄 英 李宗吉 王娅娜 赵 巍
宁夏医科大学科学与技术研究中心分子生物学实验室,银川 750004
Author(s):
SUN Jun-fengJI Huai-qingXIONG YingWANG Ya-naLI Zong-jiZHAO Wei
Medical Molecular Biology Laboratory,Ningxia Med.Univ.,Yinchuan,750004
关键词:
细粒棘球蚴 生物信息学 靶标分子 扁形动物门 高通量筛选
Keywords:
Echinococcus granulosus bioinformatics target m olecule Platyhelminthes high-throughput
分类号:
R383.3+3
DOI:
-
文献标志码:
A
摘要:
目的 用生物信息学方法从世界权威数据库检索筛选能用于诊断或治疗的细粒棘球蚴靶蛋白。方法 基于其他相近物种的寄生虫及其靶蛋白研究,在美国生物技术信息中心(NCBI)连接的SwissProt,PIR,PRF,PDB等数据库进行有价值靶蛋白及其同源蛋白的筛选,最终筛选出在其他寄生虫目前用于靶蛋白研究的蛋白或多肽,通过讨论分析,找出能作为细粒棘球蚴诊断或药物疫苗靶位的蛋白质和多肽。结果 通过检索扁形动物门的蛋白,筛选出目前该门类下所有物种已经成熟应用或正在研究的有价值的候选蛋白:共得到154条包括32种蛋白。结论 通过高通量快速筛选法,找出32种可能用于包虫病诊断和防治的靶位蛋白,为今后蛋白质组学高通量筛选细粒棘球蚴靶标分子提供理论依据和参考。
Abstract:
Objective To screen specific target proteins for early diagnosis or therapy of echinococcosis by screening the authoritative databases from all over the word with bioinformatics method.Methods Based onstudies on target proteinsof other similar species,we screened these valuable proteins or their homologous proteins from American National Center for Biotechnology Information which links with the database of proteins,such as SwissProt,PIR,PRF,PDB et al.Afterscreening out these valuable proteins or peptides of Platyhelminthes,we found some of them for Echinococcus granulosus(Eg)through analysis and demonstrations.Results We got the valuable proteins or peptides applied already or been researching on diagnosis or therapy of species of Platyhelminthes,including 154 of them that could be classified as 32 kinds.Conclusion 32 kindscandidate of proteins(peptides)were screened for Eg. diagnosis and therapy which would provide the academic basis to further proteomic researches of screening target proteins ofEg.

参考文献/References:

[1] McManus DP,Thompson RC. Molecular epidemiology of cystic echinococcosis[J]. Parasitol,2003,127(suppl): S37-51.
[2] Altschul SF,Madden TL,Schaffer AA,et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Res,1997,25:3389-3402.
[3] Abola EE,Bernstein FC,Bryant SH,et al. Protein Data Bank. In Crystallographic Databases-Information Content,Software Systems,Scientific ApplicationsEdited by: Allen FH,Bergerhoff G and Sievers R. Bonn/Cambridge/Chester[J]. Dat Commi of IUC,1987,107-132.
[4] Eddy SR. Profile hidden Markov models[J]. Bioinformatics,1998,14: 755-763.
[5] Gough J,Karplus K,Hughey R,et al. Assignment of homology to genome sequences using a library of hidden Markov models that represent all proteins of knownstructure[J]. J Mol Biol,2001,313: 903-919.
[6] The protein entries of NCBI.http://www.ncbi.nlm.nih. gov/sites/entrez?db=protein&cmd=search&term=Platyhelminthes,2009.
[7] Entrez cross-database search..http://www.ncbi.nlm. nih.gov/sites/gquery,2009.
[8] The protein entries of NCBI.http://www.ncbi.nlm.nih. gov/sites/entrez?db=protein,2009.
[9] Zhang WB,Li J,Li Q,et al. Identification of a diagnostic antibody-binding region on the immunogenic protein EpC1 from Echinococcus granulosus and its application in population screening for cystic echinococcosis[J]. Clin Exp Immunol,2007,149(1): 80-86.
[10] Zhang W,Zhang Z,Shi B,et al. Vaccination of dogs against Echinococcus granulosus,the cause of cystic hydatid disease in humans[J]. J Infect Dis,2006,194(7): 966-974.
[11] 郝慧芳,王志钢,李志伟. 细粒棘球蚴内蒙株FABP基因cDNA的克隆与核酸疫苗的构建[J]. 中国寄生虫学与寄生虫病杂志,2007,25(4): 352-354.
[12] Chow C,Gauci CG,Vural G,et al. Echinococcus granulosus: variability ofthe host-protective EG95 vaccine antigen in G6 and G7 genotypic variants[J]. Exp Parasitol,2008,119(4): 499-505.
[13] 李文桂,王鸿,朱佑明. 细粒棘球绦虫重组BCG-Eg95疫苗诱导小鼠脾细胞淋巴因子变化的研究[J]. 中国寄生虫学与寄生虫病杂志,2007,25(2): 109-113.
[14] 何莉莉,景涛,祝秉东,等. 细粒棘球绦虫Eg95重组分泌型卡介苗的构建[J]. 中国寄生虫学与寄生虫病杂志,2007,25(4): 320-324.

相似文献/References:

[1]丁淑琴,刘宏鹏,张爱君,等.生物信息学技术在细粒棘球蚴热休克蛋白70重组疫苗研究中的应用[J].宁夏医科大学学报,2009,(01):29.
 DING Shu-qin,LIU Hong-peng,ZHANG Ai-jun,et al.[J].Ningxia Medical University,2009,(02):29.
[2]杨桂茂,朱明星,张爱君,等.结核分枝杆菌宁夏分离株pst S1基因的扩增及生物信息学分析[J].宁夏医科大学学报,2010,(09):960.
 YANG Gui-mao,ZHU Ming-xing,ZHANG Ai-jun,et al.Amplification and Bioinformatic Analysis of the pst S1 Gene from Ningxia Isolated Mycobacterium Tuberculosis[J].Ningxia Medical University,2010,(02):960.
[3]雄 英,王娅娜,李宗吉,等.细粒棘球蚴中国大陆株铁蛋白生物信息学的初步分析 及其结构和功能预测[J].宁夏医科大学学报,2010,(03):327.
 XIONG Ying,WANG Ya-na,LI Zong-ji,et al.Bioinformatics Analysis for the Structure and Function of Ferritin Gene from Echinococcus Granulosus (Chinese Mainland Strain)[J].Ningxia Medical University,2010,(02):327.
[4]丁淑琴,杨园园,杨凤琴,等.结核分支杆菌重组蛋白rps12的结构与功能预测[J].宁夏医科大学学报,2012,(11):1130.
 DING Shu-qin,YANG Yuan-yuan,YANG Feng-qin,et al.Bioinformatics Analysis of the Structure and Fuction in Mycobacterium Tuberculosis Recombinant Protein rps12[J].Ningxia Medical University,2012,(02):1130.
[5]王 元,王娅娜,师志云,等.细粒棘球绦虫(中国大陆株)特异性抗原P-29 重组蛋白的生物信息学分析[J].宁夏医科大学学报,2012,(07):673.
 WANG Yuan,WANG Ya-na,SHI Zhi-yun,et al.Bioinformatics Analysis of the Echinococcus Granulosus Recombinant Ferritin Antigen[J].Ningxia Medical University,2012,(02):673.
[6]刘丽华,王元,王娅娜,等.枸杞多糖对细粒棘球蚴小鼠T淋巴细胞亚群的影响[J].宁夏医科大学学报,2012,(05):432.
 LIU Li-hua,WANG Yuan,WANG Ya-na,et al.The Effects of LBP on Lymphocyte Subsets in Echinococcus Granulosus Mice[J].Ningxia Medical University,2012,(02):432.
[7]王彦翠,张 丽,袁士珍.妊娠合并细粒棘球蚴病6例临床分析[J].宁夏医科大学学报,2012,(01):87.
[8]巨 艳,马斯明,朱明星,等.细粒棘球蚴重组HSP70蛋白免疫保护力研究[J].宁夏医科大学学报,2013,(08):850.
 JU Yan,MA Siming,ZHU Mingxing,et al.Immunoportection of Recombinant HSP70 Against Echinococcus Granulosus in Mice[J].Ningxia Medical University,2013,(02):850.
[9]朱明星,杨桂茂,孙惠敏,等.结核分枝杆菌宁夏分离株MPT64基因的生物信息学分析[J].宁夏医科大学学报,2013,(07):778.
 ZHU Ming-xing,YANG Gui-mao,SUN Hui-min,et al.The Bioinformatic Analysis of the MPT64 Gene of Mycobacterium Tuberculosis Isolated in Ningxia[J].Ningxia Medical University,2013,(02):778.
[10]王 博,丁 晓,颜 贝,等.结核分枝杆菌Ag85B、TB10.4及Ag85B-TB10.4融合基因 的克隆表达及生物信息学分析[J].宁夏医科大学学报,2013,(07):738.
 WANG Bo,DING Xiao,YAN Bei,et al.Cloning, Expression and Bioinformatics of the Ag85B,TB10.4 and Ag85B-TB10.4 Fusion Gene in Mycobacterium Tuberculosis[J].Ningxia Medical University,2013,(02):738.

备注/Memo

备注/Memo:
收稿日期:2009-12-10 基金项目:国家自然科学基金(30660176,30960360) 作者简介:孙峻峰(1982-),男,在读硕士研究生。 通信作者:赵巍,男,教授,从事分子生物学研究。
更新日期/Last Update: 2010-02-20