|本期目录/Table of Contents|

[1]杨芝红,魏军,杨宝珍.急性白血病DNA倍体、合成期细胞百分比及增殖指数的研究[J].宁夏医科大学学报,2008,(01):43-44.
 YANG Zhi hong,WEI Jun,YANG Bao zhen.A Study on DNA Ploidy,Sphase Fraction and DNA Index in Acute Leukemia[J].Ningxia Medical University,2008,(01):43-44.
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急性白血病DNA倍体、合成期细胞百分比及增殖指数的研究(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2008年01期
页码:
43-44
栏目:
论著
出版日期:
2008-01-20

文章信息/Info

Title:
A Study on DNA Ploidy,Sphase Fraction and  DNA Index in Acute Leukemia
作者:
杨芝红魏军杨宝珍
宁夏医学院附属医院医学实验中心,银川750004
Author(s):
YANG ZhihongWEI Jun YANG Baozhen
The laboratory Center of the Affiliated Hospital to Ningxia Medical College, Y inchuan 750004
关键词:
急性白血病DNA倍体合成期细胞百分比增殖指数流式 细胞仪
Keywords:
acute leukemia DNA ploidy Sphase fraction proliferation index flow cytometer
分类号:
R44611+3
DOI:
-
文献标志码:
A
摘要:
目的 观察急性白血病患者骨髓或外周血DNA倍体的变化规律。方法H 采用流式细胞仪 和DNA检测试剂盒检测急性白血病患者DNA倍体、合成期细胞百分比(SPF)、增殖指数(PI)并 与DNA倍体分析对照组进行比较。结果 52例急性白血病(AL)中,28例为 二倍体,22例为异倍 体,2例为可疑异倍体,异倍体检出率为423%,22例异倍体(1例近二倍体,5例四倍体,4 例 多异倍体和12例非整倍体)中,8例为急性淋巴细胞白血病(ALL),11例为急性髓系白血病(AM L),3例混合细胞性白血病。ALL与AML异倍体检出率分别为421%(8/19)和367%(11/30)。 异 倍体组与二倍体组及与对照组两两比较,P<005或<001,差异具有统计学意义。 结论 DNA倍体分析可为急性白血病的临床诊断提供客观的实验室依据
Abstract:
Objective To explore the changing of DNA ploidy in patients w ith acute leukamia. Methods Immunophenotype and DNA ploidy of the bone mar row and perip heral blood samples was analyzed by means of the flow cytometer with 3color fl u orescence. Results Of 52 AL patients, 28 were patients with dipl oid, 22 were p atients with heteroploid and 2 were patients with suspected heteroploid. The pre valence of heteroploid in AL patients was 42.3%. Of 22 patients with heteroploid , 8 were patients with ALL, 11 were patietns with AML and 3 were the mixed. The prevalence of heteroploid in ALL and AML were 42.1% and 36.7%, respectively. [W T5H1]Co nclusion Heteroploid group with diploid group and control group, DI, SPF and PI in diploid group and heteroploid were significantly higher than those in control g roup. Conclusions Immunologic determination is a necessary methods for diagnosi s in acute leukemia. Immunophenotype and DNA ploidy could give a scientific labo ratory basis for diagnosis, selection of treatment and prediction of prognosis.

参考文献/References:

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[4]TripathiA,Chaturvedi R,AhmadR,et alFlow cytometric analysis of aneup loi dy and S-phase fraction in chronic myeloid leukemia patients:role in early det ection of accelerated phase[J]Leuk Res,2003,27(10):899-902
[5]王卫,刘云鹏,卢香兰,等DNA含量的流式细胞仪检测在判断急性淋巴细胞白血病 预后中的意义[J].白血病淋巴瘤,2003,12(1):21-23

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备注/Memo

备注/Memo:
收稿日期:2007-07-03 作者简介: 杨芝红(1967-),女,甘肃人,副主任技师,学士,从事临床检验工作。
更新日期/Last Update: 2008-01-20