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[1]杨芝红,魏军,杨宝珍,等.三色流式细胞术对儿童急性白血病免疫表型的分析[J].宁夏医科大学学报,2005,(05):371-373.[doi:10.3969/j.issn.1674-6309.2005.05.013]
 YANG Zhi hong,WEI Jun,YANG Bao zhen,et al.Analysis on Immunophenotype of Acute Leukemiaby 3-Color Flow Cytometry in Children[J].Ningxia Medical University,2005,(05):371-373.[doi:10.3969/j.issn.1674-6309.2005.05.013]
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三色流式细胞术对儿童急性白血病免疫表型的分析(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2005年05期
页码:
371-373
栏目:
论著
出版日期:
2005-09-20

文章信息/Info

Title:
Analysis on Immunophenotype of Acute Leukemia by 3-Color Flow Cytometry in Children
作者:
杨芝红魏军杨宝珍白永泽任新明
宁夏医学院附属医院中心实验室,银川750004
Author(s):
YANG Zhihong WEI Jun YANG Baozhen et al
Laboratory Center, the Affiliated Hospital of Ningxia Medical College, Yinchuan 750004
关键词:
流式细胞仪急性白血病免疫表型
Keywords:
flow cytometer acute leukemia immunophenotype
分类号:
R557
DOI:
10.3969/j.issn.1674-6309.2005.05.013
文献标志码:
A
摘要:
目的 探讨三色荧光标记流式细胞术CD45辅助设门检测儿童急性白血病免疫表型的临床应用及意义。方法 采用流式细胞仪和几组三色荧光标记抗体检测34例FAB分型为ALL与AML的儿童骨髓或外周血免疫表型。结果 25例ALL儿童中,B型淋巴细胞白 血病14例,T型淋巴细胞白血病6例,双标记5例,在T-ALL中,淋系抗原阳性率表达顺序为CD7>CD3>CD5,在B-ALL中,淋系抗原阳性 率表达顺序为CD19>CD10>CD22>CD20,在T-ALL、B-ALL及双标记中还可同时表达髓系抗原(CD13、CD 14、CD15、CD33)。9例AML儿童中,同时或分别表达髓系标志抗原CD15、CD33、CD13、CD14, 其阳性表达率为89%、89%、79%、11%,9例中有3例还不同程度表达淋系抗原CD3、CD7、CD19。结论 三色流式细胞术,可准确将 白血病分为淋系与髓系白血病,并能将淋系白血病分为B型和T型及双标记,同时能反映髓系抗原阳性的ALL(My+ALL)与淋系抗原阳性的 AML(AL+AML),对指导临床治疗有重要价值。
Abstract:
Objective To investigate the clinical significance of immunophenotye of children acute leukemia by 3-color flow cytometry with CD45 gating Methods Immunophenotyes of bone marrow and peripheral blood samples from FAB-ALL and FAB-AML cells of 34 patients were analyzed by means of flow cytometry with 3-color fluorescence labeling antibodies Results Of the 25 patients with FAB-ALL cells, 14 were patients with B-ALL, 6 were patients with T-ALL and 5 were patients with double labeling The results showed that the antigen expression frequencies were as follows: in T-ALL, CD7>CD3 >CD5 and in B-ALL, CD19>CD10>CD22>CD20 Antigens such as CD13, CD14, CD15 , CD33 were expressed in all 3 groups and showed the same frequencies in B-ALL and T-ALL Of the 9 patients with FAB-AML cells, the antigen expression frequencies were as follows: CD15 (89%), CD33 (89%), CD13 (79%) and CD14 (11%) To different extent, 3 of 9 patients expressed antigen such as CD3, CD7 and CD19 Conclusion Three-color flow cytometry can classify the patients into B-ALL, T-ALL and double-labeling At the same time, it can express myeloid antigen ALL (My+ALL) and T antigen AML (AL+AML) This method is significant in guiding diagnosis and treatment

参考文献/References:

[1]达万明,裴雪涛现代白血病学[M]北京:人民军医出版社,2003260
[2]Jennings CD, Foon KA Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy[J]Blood,1997,90:2863-2892
[3]Stelzer GT, Shults KE, Loken MR CD45 gating for routine flow cytometric analysis of human bone marrow specimens[J] Ann NY Acad Sci,1993,77:265
[4]陈姗姗流式细胞术-白血病免疫分型规范化[J]中国实验血液学杂志, 2002,10(1):1-5
[5]王菊香,汤静燕,顾龙君,等初诊急性淋巴细胞白血病患儿髓系抗原表达及临床意义[J]中国实用儿科杂志, 2004,19(9):535- 537

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备注/Memo

备注/Memo:
收稿日期:2005-04-20 作者简介: 杨芝红(1967-),女,甘肃人,副主任技师,学士,从事临床检验工作。
更新日期/Last Update: 2005-09-20