|本期目录/Table of Contents|

[1]吴忠兰,苟明星,关光玉,等.宁夏地区艾滋病患者感染HIV-1病毒pol区基因变异性研究[J].宁夏医科大学学报,2017,(07):781-784.[doi:10.16050/j.cnki.issn1674-6309.2017.07.011]
 WU Zhonglan,GOU Mingxing,GUAN Guangyu,et al.Moleculo-epidemiological Study on HIV-1 in Ningxia[J].Ningxia Medical University,2017,(07):781-784.[doi:10.16050/j.cnki.issn1674-6309.2017.07.011]
点击复制

宁夏地区艾滋病患者感染HIV-1病毒pol区基因变异性研究(PDF)
分享到:

《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2017年07期
页码:
781-784
栏目:
论著
出版日期:
2017-07-30

文章信息/Info

Title:
Moleculo-epidemiological Study on HIV-1 in Ningxia
作者:
吴忠兰1苟明星2关光玉1尹婷3赵伟明3
1.宁夏疾病预防控制中心,银川 750004; 2. 宁夏银川市金凤区疾病预防控制中心,银川 750001; 3. 宁夏医科大学,银川 750004
Author(s):
WU Zhonglan1 GOU Mingxing2 GUAN Guangyu1 YIN Ting3 ZHAO Weiming3
1.Ningxia Center for Disease Control and Prevention, Yinchuan 750004; 2. Jin Feng center for Disease Control and Prevention in Ningxia, Yinchuan 750001; 3. Ningxia Medical University , Yinchuan 750004
关键词:
HIV-1病毒基因序列进化树艾滋病基因离散率宁夏
Keywords:
HIV-1gene sequence phylogenetic trees subtype characteristics genetic- distanceNingxia
分类号:
R512.91
DOI:
10.16050/j.cnki.issn1674-6309.2017.07.011
文献标志码:
A
摘要:
目的 通过巢式聚合酶链式反应检测宁夏地区艾滋病患者感染HIV-1病毒的pol基因变异情况,为艾滋 病防治提供参考依据。方法 采集HIV-1艾滋病患者全血标本并收集流行病学资料,6h内分离血浆,-70℃保 存待用。采用血浆提取HIV-1 RNA,针对HIV-1病毒的pol区PR基因和RT基因设计引物,巢式PCR扩增,扩增产 物经凝胶电泳鉴定,片段大小约为1.2kb,鉴定后送公司测序。结果 巢式PCR检测样本47份,成功扩增35份 样本并获得pol区序列,长1060bp。宁夏地区HIV-1感染者中存在4种基因亚型,23例CRF07_BC,6例CRF08_BC ,4例CRF01_AE和2例B亚型,基因离散率为B亚型最大。性传播的18例患者中存在3种重组亚型,CRF07_BC重 组亚型为主12例(66.7%);静脉吸毒传播的15例中存在4种重组亚型,CRF07_BC重组亚型为主11例(73.3% ),其次为B亚型2例(13.3%),CRFO1_AE重组亚型1例(6.7%)、CRF08_BC重组亚型1例(6.7%),还存在2 例性行为兼静脉吸毒的患者,属CRF01_AE重组亚型。结论 HIV-1基因型别趋于多样化,CRF07_BC重组亚型为 主要流行毒株,B亚型在宁夏地区流行时间最长;吸毒人群和性活跃人群仍是艾滋病防治的重点人群。
Abstract:
Objective To investigate the characteristics of human immunodeficicncy virus(HlV) gene sequence,sub- type distribution and origin of HlV strains, as well as its epidemic status and trends in Ningxia Province, so as to provide scientific evidence for policy making and development of AIDS prevention and control measures. Methods Anticoagulating blood samples were collected from HIV-1 infected individuals and epidemiological data were analyzed. Separation of plasma in 6 hours and -70℃ to save. Design primers according to PR and RT gene of the pol HIV-1 virus, and it was amplified by nested PCR from the RNA extraction and the amplification products is about 1.2 kb, then sequenced. The acquired sequences were compared with the international subtype references. The phylogenetic trees were constructed to determine the subtype of the samples. Results Out of the 47 samples, 35 samples were PCR positive and the sequences were acquired. 23 cases were CRF07_BC, 6 cases were CRF08_BC, 4cases were CRF01_AE, 2 cases were subtype B. The B subtype was the largest genetic-distance. There were three subtypes of 18 patients with sexually transmitted and CRF07_BC was mainly subtype, about 12 cases (66.7%). There were four subtypes of 15 cases with intravenous drug users and CRF07_BC was mainly subtype, about 11 cases (73.3%). 2 cases were subtype B (13.3%), 1 case was subtype CRFO1_AE(6.7%),and 1 case was subtype CRF08_BC(6.7%). 2 cases with sexually transmitted and intravenous drug use were subtypes CRF01_AE. Conclusion The subtype tends to diversity in Ningxia. CRF07_BC is the dominant strain and shows a high prevalence. Drug users and sex active people still are the key of the HIV/AIDS prevention and control of the crowd.

参考文献/References:

[1] Pasquier C,Millot N,Njouom R,et al. HIV-1 subtyping using phylogenetic analysis of pol gene sequences[J]. J Virol Methods,2001,94(1-2):45-54.
[2] Sung H,Foley BT,Bae IG,et al. Phylogenetic analysis of reverse transcriptase in antiretroviral drug-naive Korean HIV type 1 patients[J]. AIDS Res Hum Retroviruses,2001,17(16):1549-1554.
[3] Njouom R,Pasquier C,Sandres-Sauné K,et al. Assessment of HIV-1 subtyping for Cameroon strains using phylogenetic analysis of pol gene sequences[J]. J Virol Methods,2003,110(1):1-8.
[4] 颜瑾,王玉,李杰,等. 艾滋病病毒1型流行株分子流行病学调查[J]. 中国公共卫生,2005,21(1):90-91.
[5] 李桂英,贺雄,叶景荣,等. 北京人类免疫缺陷病毒1型阳性吸毒者env基因序列测定和亚型分析[J]. 中华预防医学杂志,2010,44(6):572-573.
[6] 李赫伟. 广西桂北地区HIV-1流行毒株的基因序列测定和亚型分析[D]. 桂林医学院,2010.
[7] 马彦民,孙定勇,李宁,等. 河南省2011年报告性途径感染HIV者的流行病学特征分析[J]. 中国艾滋病性病,2013,19(1):22-24.
[8] 邢辉,梁浩,万卓越,等. 中国CRF01_AE亚型人类免疫缺陷病毒毒株的分子流行病学研究[J]. 中华预防医学杂志,2004,38(5):300-304.
[9] Harris KA,Teo CG. Diversity of hepatitis C virus quasispecies evaluated by denaturing gradient gel electrophoresis[J]. Clinical and Vaccine Immunology,2001,8(1):62-73.

相似文献/References:

[1]杨 炬,王 雯,高 洁,等.宁夏职业暴露人群和环境高致病性禽流感感染情况调查[J].宁夏医科大学学报,2013,(03):286.
 YANG Ju,WANG Wen,GAO Jie,et al.Investigation of Avian Influenza A Virus Infection among Occupational Exposed Population of Ningxia[J].Ningxia Medical University,2013,(07):286.
[2]李海军,杨媛媛,周莉薇,等.2010年宁夏人群乙肝表面抗体特征分析[J].宁夏医科大学学报,2013,(03):280.
 LI Hai-jun,YANG Yuan-yuan,ZHOU Li-wei,et al.The Characteristics on Hepatitis B Surface-antibodyof Healthy People of Ningxia in 2010[J].Ningxia Medical University,2013,(07):280.
[3]董旭峰,雷 静,苗志峰,等.400名男男性行为人群艾滋病高危行为及其影响因素调查分析[J].宁夏医科大学学报,2013,(03):277.
 DONG Xu-feng,LEI Jing,MIAO Zhi-feng,et al.A Research of the Influencing Factors of AIDS High-risky Behavior among 400 MSM[J].Ningxia Medical University,2013,(07):277.
[4]雷 娇,张立明.宁夏与东北短梗五加不同部位黄酮含量比较[J].宁夏医科大学学报,2013,(02):157.
 LEI Jiao,ZHANG Li-ming.Comparison Study of Total Flavonoid in Parts of Acanthopanax Sessiliflorus Seem between Ningxia and Northeast Areas[J].Ningxia Medical University,2013,(07):157.
[5]周路平,吴惠忠,尤文宁,等.2011年宁夏居民碘营养现况调查[J].宁夏医科大学学报,2013,(01):55.
 ZHOU Lu-ping,WU Hui-zhong,YOU Wen-ning,et al.The Survey of Iodine Nutrition in Ningxia Residents in 2011[J].Ningxia Medical University,2013,(07):55.
[6]靳 峰,李 丽,宋晓佳,等.2010年宁夏布鲁氏菌病重点人群流行病学现况调查分析[J].宁夏医科大学学报,2013,(01):49.
 JIN Feng,LI Li,SONG Xiai-jia,et al.An Epidemiology Investigation of the Brucellosis in Key Population of Ningxia in 2010[J].Ningxia Medical University,2013,(07):49.
[7]吴忠兰,杨东智,鱼小红,等.宁夏HIV感染者CD4+T淋巴细胞计数及AIDS患者 抗病毒治疗现状分析[J].宁夏医科大学学报,2013,(01):37.
 WU Zhong-lan,YANG Dong-zhi,GUAN Guang-yu.Status Quo of Antiretroviral Therapy against HIV/AIDS Based on the CD4+T Cells Count and Virus Load in Ningxia[J].Ningxia Medical University,2013,(07):37.
[8]闫爱华,韩秋启,白 涛.吴忠市2004-2012年吸毒人群艾滋病哨点监测报告分析[J].宁夏医科大学学报,2013,(09):1008.
[9]李鸿成,慕 明,谢 峰,等.宁夏地区2006~2011年尘肺病新发病例现况分析[J].宁夏医科大学学报,2013,(09):998.
 LI Hongcheng,Mu Ming,Xie Feng,et al.Analysis on New Cases of Pneumoconiosis in Ningxia Region from 2006 to 2011[J].Ningxia Medical University,2013,(07):998.
[10]王志忠,王俊峰,张毓洪,等.宁夏地区成人失眠现况及影响因素分析[J].宁夏医科大学学报,2013,(09):994.
 WANG Zhizhong,WANG Junfeng,ZHANG Yuhong,et al.An Investigation of Insomnia Status and the Risk Factors in Ningxia[J].Ningxia Medical University,2013,(07):994.

备注/Memo

备注/Memo:
收稿日期:2016-10-12
基金项目:宁夏自然科学基金 (NZ13217) ;宁夏科技攻关计划项目国际合作专项(2013ZYH193)
作者简介:吴忠兰(1979-),女,硕士,主管检验技师,从事艾滋病检测工作。
通信作者:赵伟明(1961-),女,教授。
更新日期/Last Update: 2017-07-20