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[1]高婷,姬海玉,胡甜甜,等.过表达microRNA-126诱导INS-1细胞凋亡[J].宁夏医科大学学报,2015,(07):741-744+862.[doi:10.16050/j.cnki.issn1674-6309.2015.07.004 ]
 GAO Ting,JI Haiyu,HU Tiantian,et al.-[J].Ningxia Medical University,2015,(07):741-744+862.[doi:10.16050/j.cnki.issn1674-6309.2015.07.004 ]
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过表达microRNA-126诱导INS-1细胞凋亡(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2015年07期
页码:
741-744+862
栏目:
论著
出版日期:
2015-07-30

文章信息/Info

Title:
-
作者:
高婷; 姬海玉; 胡甜甜; 张荣华; 扈启宽; 袁文俊;
宁夏医科大学基础医学院生理学系; 第二军医大学生理学教研室;
Author(s):
GAO Ting;JI Haiyu;HU Tiantian;ZHANG Ronghua;HU Qikuan;YUAN Wenjun;Department of PhysiologySchool of Basic Medical ScienceNingxia Medical University;Department of PhysiologySecond Military Medical University;
关键词:
microRNA-126 INS-1 细胞凋亡 糖尿病
Keywords:
-
分类号:
R587.1
DOI:
10.16050/j.cnki.issn1674-6309.2015.07.004
文献标志码:
-
摘要:
目的研究过表达microRNA-126对INS-1细胞(大鼠胰岛β细胞株)增殖和凋亡的影响。方法将INS-1细胞分为4组:阴性对照(NC)组、阳性对照(miR-375)组、miR-126过表达(miR-126m)组、miR-126低表达(miR-126in)组,分别转染nonsense segment、miR-375mimic、miR-126mimic、miR-126inhibitor,48h后予胰岛素刺激细胞12h。用实时定量RT-PCR方法检测转染效果;MTS法检测INS-1细胞增殖情况;电子显微镜下观察细胞超微结构的变化。结果 miR-126m组INS-1细胞的miR-126水平较NC组升高(P<0.01),miR-126in组miR-126水平较NC组降低(P<0.05);miR-126m组及miR-375组INS-1细胞活力较NC组及miR-126in组降低(P<0.05);电镜下可见miR-126m组及miR-375组INS-1细胞大量凋亡,细胞核折缝样改变,染色质高度浓缩、边集;NC组及miR-126in组偶见凋亡细胞。结论过表达microRNA-126可以显著降低INS-1细胞活力,诱导细胞凋亡,提示microRNA-126可能与糖尿病的发生有关。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
【基金】 国家自然科学基金(81270880)
更新日期/Last Update: 1900-01-01