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[1]王成泉,王珺,尹磊,等.裸鼠体内人U343胶质瘤中Period2对胶质瘤p53的调控作用及意义[J].宁夏医科大学学报,2015,(06):620-625.[doi:10.16050/j.cnki.issn1674-6309.2015.06.004]
 WANG Chengquan,WANG Jun,YIN Lei,et al.-[J].Ningxia Medical University,2015,(06):620-625.[doi:10.16050/j.cnki.issn1674-6309.2015.06.004]
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裸鼠体内人U343胶质瘤中Period2对胶质瘤p53的调控作用及意义(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2015年06期
页码:
620-625
栏目:
论著
出版日期:
2015-06-30

文章信息/Info

Title:
-
作者:
王成泉; 王珺; 尹磊; 夏鹤春;
宁夏医科大学; 宁夏医科大学颅脑疾病重点实验室; 宁夏医科大学总医院;
Author(s):
WANG Chengquan;WANG Jun;YIN Lei;XIA Hechun;Ningxia Med. Univ.;Incubation Base of National Key Laboratory for Cerebrocranial Diseases;Dept. of Neurosurgerythe General Hospital of Ningxia Med. Univ.;
关键词:
Period2 人U343胶质瘤 MDM2 p53
Keywords:
-
分类号:
R739.4
DOI:
10.16050/j.cnki.issn1674-6309.2015.06.004
文献标志码:
-
摘要:
目的探讨裸鼠体内Period2(Per2)基因对人U343胶质瘤细胞p53的调控作用及意义。方法体外培养特异性人Per2基因shRNA慢病毒载体转染细胞(shRNA-Per2组)、非干扰慢病毒载体转染细胞(shRNAcontrol组)、空白对照U343胶质瘤细胞(空白对照组),分别接种于裸鼠颈后皮下,处于标准饲养环境成瘤。qRT-PCR、Western blot技术分别检测shRNA-Per2组、shRNA-control组、空白对照组中胶质瘤Per2、MDM2、p53、ATM、c-myc的mRNA和蛋白含量。结果 Per2低表达加快裸鼠体内胶质瘤细胞的成瘤及肿瘤的生长,shRNA-Per2组胶质瘤中Per2、p53、ATM的mRNA含量均低于shRNA-control组和空白对照组(P均<0.05);而MDM2与c-myc的mRNA水平均高于shRNA-control组和空白对照组(P均<0.05);各个基因的空白对照组与shRNA-control组中mRNA表达差异无统计学意义(P均>0.05)。Western blot结果表明各组裸鼠体内胶质瘤中5种蛋白的表达与上述mRNA呈现相同的趋势。shRNA-Per2组胶质瘤中Per2蛋白表达(0.541±0.0516)低于shRNA-control组(0.854±0.0947)和空白对照组(0.787±0.0913)(P均<0.05),p53蛋白表达(0.707±0.126)低于shRNA-control组(1.160±0.0717)和空白对照组(1.300±0.105)(P均<0.05),ATM表达(0.364±0.0309)低于shRNA-control组(0.742±0.190)和空白对照组(0.719±0.175)(P均<0.05),而shRNA-Per2组胶质瘤中MDM2蛋白表达(0.777±0.145)高于shRNA-control组(0.520±0.0634)和空白对照组(0.483±0.096)(P均<0.05),c-myc蛋白表达(0.522±0.0624)高于shRNA-control组(0.280±0.0652)和空白对照组(0.237±0.0623)(P均<0.05)。而各个基因在空白对照组与shRNA-control组中相对蛋白表达水平差异无统计学意义(P均>0.05)。结论在胶质瘤发生发展中Per2基因促进p53和ATM的表达,降低MDM2和c-myc的表达;Per2可视为今后治疗胶质瘤的潜在靶点。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
【基金】 国家自然科学基金(81160313)
更新日期/Last Update: 1900-01-01