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[1]昝海英,刘 爽,武慧娟,等.不同浓度硫氢化钠对人脐静脉平滑肌细胞生长的影响[J].宁夏医科大学学报,2013,(08):856-859.
 ZAN Haiying,LIU Shuang,WU Huijuan,et al.Effect of Different Concentrations of Sodium Hydrosulfide on Human Umbilical Vein Smooth Muscle Cells[J].Ningxia Medical University,2013,(08):856-859.
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不同浓度硫氢化钠对人脐静脉平滑肌细胞生长的影响(PDF)
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年08期
页码:
856-859
栏目:
论著
出版日期:
2013-08-20

文章信息/Info

Title:
Effect of Different Concentrations of Sodium Hydrosulfide on Human Umbilical Vein Smooth Muscle Cells
作者:
昝海英1 刘 爽2 武慧娟3 张鸣号3 徐 华3 杨晓玲3 贾月霞3 曹 军3 李桂忠3
1.宁夏医科大学,银川 750004; 2.山东滨州医学院医学研究中心,烟台 264003; 3.宁夏医科大学基础医学院病理生理学系,银川 750004
Author(s):
ZAN Haiying1 LIU Shuang2 WU Huijuan1 ZHANG Minghao1 XU Hua1 YANG Xiaoling1 JIA Yuexia1 CAO Jun1 LI Guizhong1
1.Department of Pathophysiology,School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004; 2.Medical Research Center of Binzhou Medical College, Yantai 264003
关键词:
人脐静脉平滑肌细胞 硫氢化钠 细胞培养
Keywords:
human umbilical vein smooth muscle cell sodium hydrosulfide
分类号:
Q253
DOI:
-
文献标志码:
A
摘要:
目的 摸索体外培养体系中不影响人脐静脉平滑肌细胞(HUVSMCs)生长的硫氢化钠(NaHS)适宜浓度。方法 采用原代培养人脐静脉平滑肌细胞,实验分为八组,分别是正常对照组和七个NaHS浓度组,依次为:5.0×10-3M、1.0×10-3M、2.0×10-4M、4.0×10-5M、8.0×10-6M、1.6×10-6M、3.2×10-7M。采用细胞计数法绘制细胞生长曲线并观察细胞的生长状态,MTT检测细胞生长活力,流式细胞术检测细胞周期并进行比较。结果 4.0×10-5M组、8.0×10-6M组、1.6×10-6M组、3.2×10-7M组从细胞生长活力、细胞周期及细胞生长曲线绘制三方面与对照组相比,差异均无统计学意义(P>0.05); 5.0×10-3M组、1.0×10-3M组、2.0×10-4M组与对照组相比,细胞生长受到抑制,差异有统计学意义(P<0.05)。结论 1.6×10-6M~4.0×10-5M中任意浓度的NaHS均不影响HUVSMCs的正常生长,其可作为培养HUVSMCs并作为实验材料的浓度范围。
Abstract:
Objective To explore the optimal concentration of NaHS for primary cultured human umbilical vein smooth muscle cells(HUSMCs)growing. Methods Culturing human umbilical vein smooth muscle cells were divided into eight different concentrations: normal control group(culture medium without NaHS),5.0×10-3M group(culture medium containing 5.0×10-3M NaHS),1.0×10-3M group(culture medium containing 1.0×10-3M NaHS),2.0×10-4M group(culture medium containing 2.0×10-4M NaHS), 4.0×10-5M group(culture medium containing 4.0×10-5M NaHS), 8.0×10-6M group(culture medium containing 8.0×10-6M NaHS), 1.6×10-6M group(culture medium containing 1.6×10-6M NaHS), 3.2×10-7M group(culture medium containing 3.2×10-7M NaHS). Cell growth activity was detected by MTT. Cell cycle was determined by flow cytometry and the cell growth curve was draw. Results The cell cycle and the plateau density in 4.0×10-5M, 8.0×10-6M, 1.6×10-6M, 3.2×10-7M showed no different from those in control group(P>0.05). Compared with the control group, Cell growth was significantly inhibited in 5.0×10-3M,1.0×10-3M, 2.0×10-4M(P<0.05). Conclusion 1.6×10-6M~4.0×10-5M of NaHS can be used as the optimal concentration range in HUSMCs growth.

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相似文献/References:

[1]武慧娟,李桂忠.外源性硫化氢通过对抗氧化应激减轻血管平滑肌细胞钙化[J].宁夏医科大学学报,2015,(07):764.[doi:10.16050/j.cnki.issn1674-6309.2015.07.010 ]
 WU Huijuan,LI Guizhong,Pathophysiology Department,et al.[J].Ningxia Medical University,2015,(08):764.[doi:10.16050/j.cnki.issn1674-6309.2015.07.010 ]

备注/Memo

备注/Memo:
收稿日期:2013-03-07 基金项目:宁夏自然科学基金(NZ09105) 作者简介:昝海英(1984-),女,山西人,在读硕士研究生。 通信作者:李桂忠(1965-),男,副教授,从事心血管病理生理学研究工作。E-mail:lilylove36@163.com
更新日期/Last Update: 2013-08-20