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[1]陈 杰,张 宁,王燕蓉,等.小鼠卵巢玻璃化冻存过程中FSH干预对 VEGF及VEGFR-2表达的影响[J].宁夏医科大学学报,2013,(03):236-239,243.
 CHEN Jie,ZHANG Ning,WANG Yan-rong,et al.The Influence of FSH Interpose on the VEGF and VEGFR-2 Expressionin the Different Stage of Mouse Ovary Vitrification[J].Ningxia Medical University,2013,(03):236-239,243.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2013年03期
页码:
236-239,243
栏目:
论著
出版日期:
2013-03-20

文章信息/Info

Title:
The Influence of FSH Interpose on the VEGF and VEGFR-2 Expression in the Different Stage of Mouse Ovary Vitrification
作者:
陈 杰12 张 宁1 王燕蓉12 裴秀英1 黑常春12 马会明12 常 青12 马文智12 蔡玉芳12 孙 苗1
1.宁夏医科大学生育力保持教育部重点实验室、宁夏生殖与遗传重点实验室,银川 750004; 2.宁夏医科大学人体解剖与组织胚胎学系,银川 750004
Author(s):
CHEN Jie12 ZHANG Ning1 WANG Yan-rong12 PEI Xiu-ying1 HEI Chang-chun12 MA Hui-ming12 CHANG Qing12 MA Wen-zhi12 CAI Yu-fang12 SUN Miao1
1. Key Laboratory of Fertility Maintenance, Ministry of Education, Ningxia Medical University & Key Laboratory of Reproduction and Genetics, Yinchuan 750004; 2.Department of Human Anatomy and Histoembryology, Ningxia Medical University,Yinchuan 750004
关键词:
小鼠 卵巢 FSH 玻璃化冻存 VEGF VEGFR-2
Keywords:
mouse ovary FSH vitrification VEGF VEGFR-2
分类号:
Q954.3
DOI:
-
文献标志码:
A
摘要:
目的 通过在玻璃化冻存不同时段给予小鼠卵巢组织重组人促卵泡刺激素(rFSH)干预,观察rFSH对VEGF以及VEGFR-2表达的影响,探索卵巢玻璃化冻存过程中最佳的FSH保护途径。方法 将4周龄C57BL/6J小鼠卵巢分为新鲜对照组(FCG)、玻璃化冻存对照组(VCG)、0.3IU·mL-1 FSH干预玻璃化冻存组,包括全程添加FSH组(OG-FSH)、冻存前添加FSH组(BG-FSH)以及冻存后添加FSH组(AG-FSH)。通过形态学技术观察正常卵泡百分比; 采用免疫组织化学技术观察卵巢不同细胞VEGF以及VEGFR-2的表达; 通过Western-blot方法观察并分析不同玻璃化冻存时段的FSH干预对卵巢VEGF以及VEGFR-2蛋白表达量的影响。结果 正常卵泡百分比、VEGF及VEGFR免疫组织化学和Western-blot检测结果组间比较差异均有统计学意义。其中,OG-FSH组各项检测指标均高于其他各组(P<0.05),且BG-FSH、AG-FSH组与FCG相似,高于VCG组(P<0.05)。结论 玻璃化冻存全程添加FSH干预有利于提高正常卵泡数,以及VEGF、VEGFR-2蛋白的表达。
Abstract:
Objective To investigate the best protection way of FSH, which interposed in different stage of vitrification process via observing the expression of VEGF and VEGFR-2. Methods:4-week-old C57BL/6J mice were divided into fresh control group(FCG),vitrification control group(VCG)and the vitrification intervention group by 0.3IU·mL-1 FSH(VIG). VIG included the group of FSH interposed in the overall process of vitrification group(OG-FSH), FSH interposed in the before process of vitrification group(BG-FSH)and FSH interposed in the after process of vitrification group(AG-FSH).The percentage of normal follicles were observed through morphology and, the expression of VEGF,VEGFR-2 in different cells of ovary were observed by immunohistochemical technique. The effect on the expression of VEGF,VEGFR-2 protein about FSH intervention in different stage of vitrification was observed and analyzed by western-blot. Results Normal follicles,VEGF and VEGFR-2. in OG-FSH were significantly higher than those in other groups(P<0.05). Conclusion OG-FSH is in favour of improving the percentage of normal follicles and the expression of VEGF,VEGFR-2 protein.

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备注/Memo

备注/Memo:
收稿日期:2012-11-26 基金项目:国家自然科学基金资助项目(81160085); 宁夏回族自治区自然科学基金重点项目(NZ1084); 宁夏教育厅重点项目(宁教高[2011]263) 作者简介:陈杰(1984-),女,内蒙古人,在读硕士研究生,从事生殖工程专业。 通信作者:王燕蓉,山东大学联合博士研究生导师。 E-mail:4083304@163.com
更新日期/Last Update: 2013-03-20