|本期目录/Table of Contents|

[1]丁淑琴,杨园园,杨凤琴,等.结核分支杆菌重组蛋白rps12的结构与功能预测[J].宁夏医科大学学报,2012,(11):1130-1132.
 DING Shu-qin,YANG Yuan-yuan,YANG Feng-qin,et al.Bioinformatics Analysis of the Structure and Fuction in Mycobacterium Tuberculosis Recombinant Protein rps12[J].Ningxia Medical University,2012,(11):1130-1132.
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《宁夏医科大学学报》[ISSN:1005-8486/CN:64-1029/R]

卷:
期数:
2012年11期
页码:
1130-1132
栏目:
论著
出版日期:
2012-11-30

文章信息/Info

Title:
Bioinformatics Analysis of the Structure and Fuction in Mycobacterium Tuberculosis Recombinant Protein rps12
作者:
丁淑琴1 杨园园2 杨凤琴1 朱佳佳1
1.宁夏医科大学,银川 750004; 2.宁夏银川市第一人民医院,银川 750001
Author(s):
DING Shu-qin1 YANG Yuan-yuan2 YANG Feng-qin1 ZHU Jia-jia1
1.Ningxia Medical University, Yinchuan 750004; 2.Yinchuan First People's Hospital,Yinchuan 750001
关键词:
结核分支杆菌 rps12 生物信息学 结构 功能
Keywords:
Mycobacterium tuberculosis rps12 bioinformatics structure function
分类号:
Q518.4
DOI:
-
文献标志码:
A
摘要:
目的 应用生物信息学分析软件预测结核分支杆菌重组抗原rps12的结构与功能。方法 应用NCBI、Expasy等在线生物信息学网站及DNAstar、Rasmol等软件包分析预测结核分支杆菌重组蛋白rps12的二级结构、三级结构; 预测主要抗原表位等。结果 经预测,该蛋白分子质量约为13849.2KDa,理论等电点为11.37, 有6个抗原表位,其结构域位于1-34,35-124。结论 生物信息学技术在结核分支杆菌rps12重组蛋白研究中有一定的理论和参考价值。
Abstract:
Objective To predict the structure and function of recombinant antigen rps12 of Mycobacterium tuberculosis using bioinformatics method. Methods Multi-sequence homological alignment, secondary structure and tertiary structure,antigenic epitope analysis were performed by online analysis at bioinformatics websites such as NCBI(http://www.nibi.nlm.nih.gov/)and Expasy(http://cn.expasy. org/), and employing software packages such as DNAstar and Rasmol. Results Analysis of the predicted protein showed that the molecular mass was 13849.2KDa, PI was 11.37, function sites were 1-34, 35-124 and six antigen pitopes were found. Conclusion Bioinformatic prediction is an effective method to find and analyze the rps12 gene of mycobacterium tuberculosis recombinant protein.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2012-01-05 基金项目:宁夏自然基金项目(NZ1196); 宁夏医科大学校级项目基金(XM200825,XM201012) 作者简介:丁淑琴(1974-),女(回族),讲师,硕士,宁夏医科大学检验学院。E-mail:md-dshn@163.com
更新日期/Last Update: 2012-11-20